Spontaneous in vitro transformation of primary human osteoblast-like cells

Cancer Genomics and Proteomics

Volumn 7, Issue 2, 2010, Pages 61-66

  Magdolen, Ursula ^a   Schmitt, Manfred ^a   Hildebrandt, Barbara ^b   Diehl, Peter ^c   Schauwecker, Johannes ^a   Saldamli, Belma ^a   Burgkart, Rainer ^a   Tübel, Jutta ^a   Gradinger, Rainer ^a   Royer Pokora, Brigitte ^b  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^b HEINRICH HEINE UNIVERSITY   (Germany)

^c UNIVERSITY OF ROSTOCK   (Germany)

Author keywords

Biosafety; Chromosome rearrangement; Immortalization; Primary human osteoblast like cells

Indexed keywords

AGAROSE; ALKALINE PHOSPHATASE;

ANCHORAGE INDEPENDENT GROWTH; ARTICLE; BIOSAFETY; CARCINOGENESIS; CELL PROLIFERATION; CELL STRUCTURE; CELL THERAPY; CELL TRANSFORMATION; CONTROLLED STUDY; CULTURE MEDIUM; HISTOLOGY; HUMAN; HUMAN CELL; HUMAN TISSUE; IN VITRO STUDY; KARYOTYPING; OSTEOBLAST; TISSUE ENGINEERING;

EID: 77950940528     PISSN: 11096535     EISSN: 17906245     Source Type: Journal    
DOI: None     Document Type: Article

References (20)

1. Kneser U, Schaefer DJ, Polykandriotis E and Horch RE: Tissue engineering of bone: the reconstructive surgeon's point of view. J Cell Mol Med 10: 695-707, 2006.
2. Allen-Hoffmann BL, Schlosser SJ, Ivarie CA, Sattler CA, Meisner LF and O'Connor SL: Normal growth and differentiation in a spontaneously immortalized near-diploid human keratinocyte cell line, NIKS. J Invest Dermatol 114: 444-455, 2000.
3. Soule HD, Maloney TM, Wolman SR, Peterson WD Jr, Brenz R, McGrath CM, Russo J, Pauley RJ, Jones RF and Brooks SC: Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res 50: 6075-6086, 1990.
4. Takahashi K, Sawasaki Y, Hata J, Mukai K and Goto T: Spontaneous transformation and immortalization of human endothelial cells. In Vitro Cell Dev Biol 26: 265-274, 1990.
5. Zhang J, Wang X, Zhao Y, Chen B, Suo G and Dai J: Neoplastic transformation of human diploid fibroblasts after long-term serum starvation. Cancer Lett 243: 101-108, 2006.
6. Rubio D, Garcia-Castro J, Martin MC, de la Fuente R, Cigudosa JC, Lloyd AC and Bernad A: Spontaneous human adult stem cell transformation. Cancer Res 65: 3035-3039, 2005.
7. Maitra A, Arking DE, Shivapurkar N, Ikeda M, Stastny V, Kassauei K, Sui G, Cutler DJ, Liu Y, Brimble SN, Noaksson K, Hyllner J, Schulz TC, Zeng X, Freed WJ, Crook J, Abraham S, Colman A, Sartipy P, Matsui S, Carpenter M, Gazdar AF, Rao M and Chakravarti A: Genomic alterations in cultured human embryonic stem cells. Nat Genet 37: 1099-1103, 2005.
8. Landegren U: Measurement of cell numbers by means of the endogenous enzyme hexosaminidase. Applications to detection of lymphokines and cell surface antigens. J Immunol Methods 67: 379-388, 1984.
9. Shaffer LG and Tommerup N (eds.). An International System For Human Cytogenetic Nomenclature. Basel: Karger S, 2005.
10. Riedel H: Models for Tumour Growth and Differentiation. The Cancer Handbook 2. pp. 1-20, 2002, London: Nature Publishing Group.
11. Aaronson SA: Growth factors and cancer. Science 254: 1146-1153, 1991.
12. Kassem M, Burns JS, Garcí́a Castro J and Rubio Muñoz D: Adult stem cells and cancer. Cancer Res 65: 9601, 2005.
13. Atiye J, Wolf M, Kaur S, Monni O, Bohling T, Kivioja A, Tas E, Serra M, Tarkkanen M and Knuutila S: Gene amplifications in osteosarcoma-CGH microarray analysis. Genes Chromosomes Cancer 42: 158-163, 2005.
14. Bridge JA, Nelson M, McComb E, McGuire MH, Rosenthal H, Vergara G, Maale GE, Spanier S and Neff JR: Cytogenetic findings in 73 osteosarcoma specimens and a review of the literature. Cancer Genet Cytogenet 95: 74-87, 1997.
15. Hattinger CM, Reverter-Branchat G, Remondini D, Castellani GC, Benini S, Pasello M, Manara MC, Scotlandi K, Picci P and Serra M: Genomic imbalances associated with methotrexate resistance in human osteosarcoma cell lines detected by comparative genomic hybridization-based techniques. Eur J Cell Biol 82: 483-493, 2003.
16. Rochet N, Dubousset J, Mazeau C, Zanghellini E, Farges MF, de Novion HS, Chompret A, Delpech B, Cattan N, Frenay M and Gioanni J: Establishment, characterisation and partial cytokine expression profile of a new human osteosarcoma cell line (CAL 72). Int J Cancer 82: 282-285, 1999.
17. Frosina G: Counteracting spontaneous transformation via overexpression of rate-limiting DNA base excision repair enzymes. Carcinogenesis 22: 1335-1341, 2001.
18. Busuttil RA, Rubio M, Dolle ME, Campisi J and Vijg J: Oxygen accelerates the accumulation of mutations during the senescence and immortalization of murine cells in culture. Aging Cell 2: 287-294, 2003.
19. Rossi O, Barbieri O and Frosina G: Time-course of spontaneous transformation of CD-1 mouse embryonic fibroblasts. Anticancer Res 23: 1373-1377, 2003.
20. Zhou YF, Bosch-Marce M, Okuyama H, Krishnamachary B, Kimura H, Zhang L, Huso DL and Semenza GL: Spontaneous transformation of cultured mouse bone marrow-derived stromal cells. Cancer Res 66: 10849-10854, 2006.