Cholesterol-dependent macropinocytosis and endosomal escape control the transfection efficiency of lipoplexes in CHN living cells

Molecular Pharmaceutics

Volumn 9, Issue 2, 2012, Pages 334-340

  Cardarelli, Francesco ^a   Pozzi, Daniela ^b   Bifone, Angelo ^a   Marchini, Cristina ^c   Caracciolo, Giulio ^b  

^a CENTER FOR NANOTECHNOLOGY INNOVATION NEST   (Italy)

^b SAPIENZA UNIVERSITY OF ROME   (Italy)

^c UNIVERSITY OF CAMERINO   (Italy)

Author keywords

cationic liposomes; cell transfection; endocytosis; endosomal escape; lipoplexes; lysosome degradation; macropiocytosis

Indexed keywords

1,2 DIOLEOYL 3 TRIMETHYLAMMONIOPROPANE; 3BETA [N (N,N DIMETHYLAMINOETHANE) CARBAMOYL] CHOLESTEROL; AMPHOLYTE; CHOLESTEROL; CLATHRIN; DIOLEOYLPHOSPHATIDYLETHANOLAMINE; DIOLEOYLPHOSPHOCHOLINE; LIPOPLEX; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CAVEOLA; CELL FATE; CELLULAR DISTRIBUTION; CHO CELL; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; ENDOCYTOSIS; ENDOSOMAL ESCAPE; ENDOSOME; FEMALE; GENE DELIVERY SYSTEM; GENETIC TRANSFECTION; INTRACELLULAR TRANSPORT; LYSOSOME; MACROPINOCYTOSIS; NONHUMAN; PHYSICAL CHEMISTRY; PINOCYTOSIS; PRIORITY JOURNAL;

ANIMALS; CHO CELLS; CHOLESTEROL; CRICETINAE; CRICETULUS; DNA; ENDOSOMES; FATTY ACIDS, MONOUNSATURATED; FLUORESCENT DYES; LIPOSOMES; MICROSCOPY, CONFOCAL; PHOSPHATIDYLCHOLINES; PHOSPHATIDYLETHANOLAMINES; PINOCYTOSIS; QUATERNARY AMMONIUM COMPOUNDS; TRANSFECTION;

EID: 84856854448     PISSN: 15438384     EISSN: 15438392     Source Type: Journal    
DOI: 10.1021/mp200374e     Document Type: Article

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