Functional expression of recombinant anti-BNP scFv in methylotrophic yeast Pichia pastoris and application as a recognition molecule in electrochemical sensors

World Journal of Microbiology and Biotechnology

Volumn 28, Issue 3, 2012, Pages 1027-1034

  Maeng, Bo Hee ^a   Choi, Jeongyeon ^a   Sa, Young Seung ^a   Shin, Jae Ho ^a   Kim, Yong Hwan ^a  

^a Kwangwoon University   (South Korea)

Author keywords

B type natriuretic peptide; Electrochemical sensor; Pichia pastoris; ScFv

Indexed keywords

ANALYTICAL PERFORMANCE; ATRIAL NATRIURETIC PEPTIDES; B-TYPE NATRIURETIC PEPTIDE; BINDING ACTIVITIES; CARCINOEMBRYONIC ANTIGEN; CULTURE CONDITIONS; DETECTION LIMITS; ELECTROCHEMICAL IMMUNOASSAYS; EUKARYOTIC EXPRESSION; FUNCTIONAL EXPRESSION; HEART FAILURE; HUMAN SERUM ALBUMINS; INCUBATION TEMPERATURES; LINEAR DETECTION; METHYLOTROPHIC YEASTS; N-TERMINALS; P. PASTORIS; PH RANGE; PICHIA PASTORIS; SCFV; SINGLE CHAIN VARIABLE FRAGMENTS; SPECIFIC BINDING;

CARDIOLOGY; ELECTROCHEMICAL SENSORS; PEPTIDES; TELEMEDICINE; YEAST;

ANTIGENS;

BRAIN NATRIURETIC PEPTIDE; RECOMBINANT PROTEIN;

ARTICLE; BLOOD; DIAGNOSTIC PROCEDURE; ELECTROCHEMICAL ANALYSIS; EVALUATION; GENE EXPRESSION; GENETICS; HEART FAILURE; HUMAN; IMMUNOASSAY; IMMUNOGLOBULIN VARIABLE REGION; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; PICHIA; SENSITIVITY AND SPECIFICITY;

CLINICAL LABORATORY TECHNIQUES; ELECTROCHEMICAL TECHNIQUES; GENE EXPRESSION; HEART FAILURE; HUMANS; IMMUNOASSAY; IMMUNOGLOBULIN VARIABLE REGION; NATRIURETIC PEPTIDE, BRAIN; PICHIA; RECOMBINANT PROTEINS; SENSITIVITY AND SPECIFICITY;

EUKARYOTA; PICHIA PASTORIS;

EID: 84856662819     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11274-011-0901-5     Document Type: Article

References (39)

1. Bach H, Mazor Y, Shaky S, Shoham-Lev A, Berdichevsky Y, Gutnick DL, Benhar I (2001) Escherichia coli maltose-binding protein as a molecular chaperone for recombinant intracellular cytoplasmic single-chain antibodies. J Mol Biol 312: 79-93.
2. Batra G, Gurramkonda C, Nemani SK, Jain SK, Swaminathan S, Khanna N (2010) Optimization of conditions for secretion of dengue virus type 2 envelope domain III using Pichia pastoris. J Biosci Bioeng 110: 408-414.
3. Brophy SE, Tyner JD, Tu BX, Pinkus MS, Shin JW (2007) Human BNP immunospecific antibodies. US Patent 2007/0207152 A1.
4. Cardarelli R, Lumicao TG Jr (2003) B-type natriuretic peptide: a review of its diagnostic, prognostic, and therapeutic monitoring value in heart failure for primary care physicians. J Am Board Fam Pract 16: 327-333.
5. Chang HJ, Choi SW, Chun HS (2008) Expression of functional single-chain variable domain fragment antibody (scFv) against mycotoxin zearalenone in Pichia pastoris. Biotechnol Lett 30: 1801-1806.
6. Chauhan AK, Arora D, Khanna N (1999) A novel feeding strategy for enhanced protein production by fed-batch fermentation in recombinant Pichia pastoris. Process Biochem 34: 139-145.
7. Crepin VF, Faulds CB, Connerton IF (2003) Production and characterization of the Talaromyces stipitatus feruloyl esterase FAEC in Pichia pastoris: identification of the nucleophilic serine. Protein Expr Purif 29(2): 176-184.
8. Dragosits M, Stadlmann J, Albiol J, Baumann K, Maurer M, Gasser B, Sauer M, Altmann F, Ferrer P, Mattanovich D (2009) The effect of temperature on the proteome of recombinant Pichia pastoris. J Proteome Res 8: 1380-1392.
9. Ecamilla-Treviño LL, Viader-Salvad'o JM, Barrera-Saldaña HA, Guerrero-Olazar'an M (2000) Biosynthesis and secretion of recombinant human growth hormone in Pichia pastoris. Biotechnol Lett 22: 109-114.
10. Eurwilaichitr L, Roytrakul S, Suprasongsin C, Manitchotpisit P, Panyim S (2002) Glutamic acid and alanine spacer is not necessary for removal of MF α-1 signal sequence fused to the human growth hormone produced from Pichia pastoris. World J Microbiol Biotechnol 18: 493-498.
11. Ghosalkar A, Sahai V, Srivastava A (2008) Secretory expression of interferon-alpha 2b in recombinant Pichia pastoris using three different secretion signals. Protein Expr Purif 60: 103-109.
12. Goda S, Takano K, Yamagata Y, Katakura Y, Yutani K (2000) Effect of extra N-terminal residues on the stability and folding of human lysozyme expressed in Pichia pastoris. Protein Eng 13(4): 299-307.
13. Grantham JA, Borgeson DD, Burnett JC (1997) BNP: pathophysiological and potential therapeutic roles in acute congestive heart failure. Am J Physiol 92: 1077-1083.
14. Hammerer-Lercher A, Halfinger B, Sarg B, Mair J, Puschendorf B, Griesmacher A, Guzman NA, Lindner HH (2008) Analysis of circulating forms of proBNP and NT-proBNP in patients with severe heart failure. Clin Chem 54: 858-865.
15. Hayden MS, Gilliland LK, Ledbetter JA (1997) Antibody engineering. Curr Opin Immunol 9: 201-212.
16. Ionescu RE, Jaffrezic-Renault N, Bouffier L, Gondran C, Cosnier S, Pinacho DG, Marcoc MP, S'anchez-Baeza FJ, Healy T, Martelet C (2007) Impedimetric immunosensor for the specific label free detection of ciprofloxacin antibiotic. Biosens Bioelectron 23: 549-555.
17. Kim TR, Goto Y, Hirota N, Kuwata K, Denton H, Wu SY, Sawyer L, Batt CA (1997) High-level expression of bovine β-lactoglobulin in Pichia pastoris and characterization of its physical properties. Protein Eng 10(11): 1339-1345.
18. Kurita R, Yokota Y, Sato Y, Mizutani F, Niwa O (2006) On-chip enzyme immunoassay of a cardiac marker using a microfluidic device combined with a portable surface plasmon resonance system. Anal Chem 78: 5525-5531.
19. Li Z, Xiong F, Lin Q, d'Anjou M, Daugulis AJ, Yang DSC, Hew CL (2001) Low-temperature increases the yield of biologically active herring antifreeze protein in Pichia pastoris. Protein Expr Purif 21: 438-445.
20. Li T, Cheng J, Hu B, Liu Y, Qian G, Liu F (2008) Construction, production, and characterization of recombinant scFv antibodies against methamidophos expressed in Pichia pastoris. World J Microbiol Biotechnol 24: 867-874.
21. Lin J, Ju H (2005) Electrochemical and chemiluminescent immunosensors for tumor markers. Biosens Bioelectron 20: 1461-1470.
22. Longenecker KL, Ruan Q, Fry EH, Saldana SC, Brophy SE, Richardson PL, Tetin SY (2009) Crystal structure and thermodynamic analysis of diagnostic mAb 106. 3 complexed with BNP 5-13 (C10A). Proteins 76: 536-547.
23. Macauley-Patrick S, Fazenda ML, McNeil B, Harvey LM (2005) Heterologous protein production using the Pichia pastoris expression system. Yeast 22: 249-270.
24. Maeng BH, Nam DH, Kim YH (2011) Coexpression of molecular chaperones to enhance functional expression of anti-BNP scFv in the cytoplasm of Escherichia coli for the detection of B-type natriuretic peptide. World J Microbiol Biotechnol 27: 1391-1398.
25. McDonnell B, Hearty S, Leonard P, O'Kennedy R (2009) Cardiac biomarkers and the case for point-of-care testing. Clinical Biochem 42: 549-561.
26. Morrison LK, Harrison A, Krishnaswamy P, Kazanegra R, Clopton P, Maisel A (2002) Utility of a rapid B-natriuretic peptide assay in differentiating congestive heart failure from lung disease in patients presenting with dyspnea. J Am Coll Cardio 39(2): 202-209.
27. Mueller C, Scholer A, Laule-Kilian K, Martina B, Schindler C, Buser P, Pfisterer M, Perruchoud AP (2004) Use of B-type natriuretic peptide in the evaluation and management of acute dyspnea. N Engl J Med 350: 647-654.
28. Mukoyama M, Nakao K, Hosoda K, Suga S, Saito Y, Ogawa Y, Shirakami G, Jougasaki M, Obata K, Yasue H, Kambayashi Y, Inouye K, Imura H (1991) Brain natriuretic peptide as a novel cardiac hormone in humans evidence for an exquisite dual natriuretic peptide system, atrial natriuretic peptide and brain natriuretic peptide. J Clin Invest 87(4): 1402-1412.
29. Palecek E, Kizek R, Havran L, Billova S, Fojta M (2002) Electrochemical enzyme-linked immunoassay in a DNA hybridization sensor. Anal Chim Acta 469: 73-83.
30. Polevoda B, Sherman F (2003) N-terminal acetyltransferases and sequence requirements for N-terminal acetylation of eukaryotic proteins. J Mol Biol 325: 595-622.
31. Seferian KR, Tamm NN, Semenov AG, Mukharyamova KS, Tolstaya AA, Koshkina EV, Kara AN, Krasnoselsky MI, Apple FS, Esakova TV, Filatov VL, Katrukha AG (2007) The brain natriuretic peptide (BNP) precursor is the major immunoreactive form of BNP in patients with heart failure. Clinical Chem 53: 866-873.
32. Shi X, Karkut T, Chamankhah M, Alting-Mees M, Hemmingsen SM, Hegedus D (2003) Optimal conditions for the expression of a single-chain antibody (scFv) gene in Pichia pastoris. Protein Expr Purif 28: 321-330.
33. Sreekrishna K, Brankamp RG, Kropp KE, Blankenship DT, Tsay J, Smith PL, Wierschke JD, Subramaniam A, Birkenberger LA (1997) Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris. Gene 190: 55-62.
34. Tanfous NGB, Kallel H, Jarboui MA, Fathallah DM (2006) Expression in Pichia pastoris of a recombinant scFv form of MAb 107, an anti human CD11b integrin antibody. Enzyme Microb Technol 38: 636-642.
35. Vedvick T, Buckholz RG, Engel M, Urcan M, Kinney J, Provow S, Siegel RS, Thill GP (1990) High-level secretion of biologically active aprotinin from the yeast Pichia pastoris. J Ind Microbiol 7: 197-202.
36. Wang J, Ibanez A, Chatrathi MP, Escarpa A (2001) Electrochemical enzyme immunoassays on microchip platforms. Anal Chem 73: 5323-5327.
37. Wang J, Xu D, Erdem A, Polsky R, Salazar MA (2002) Genomagnetic electrochemical assays of DNA hybridization. Talanta 56: 931-938.
38. Wang J, Ibanez A, Chatrathi MP (2003) On-chip integration of enzyme and immunoassays: simultaneous measurements of insulin and glucose. J Am Chem Soc 125: 8444-8445.
39. Wellcome G (1995) Advances in the use of Pichia pastoris for high-level gene expression. Curr Opin Biotechnol 6: 527-533.