Construction, production, and characterization of recombinant scFv antibodies against methamidophos expressed in Pichia pastoris

World Journal of Microbiology and Biotechnology

Volumn 24, Issue 6, 2008, Pages 867-874

  Li, Tiejun ^a   Cheng, Jianren ^a   Hu, Baishi ^a   Liu, Yan ^a   Qian, Guoliang ^a   Liu, Fengquan ^a  

^a NANJING AGRICULTURAL UNIVERSITY   (China)

Author keywords

Expression; Immunochemical characterization; Methamidophos; Pichia pastoris; ScFv

Indexed keywords

AGRICULTURE; ANTIBODIES; CELL CULTURE; CLONING;

IMMUNOCHEMICAL CHARACTERIZATION; METHAMIDOPHOS; PICHIA PASTORIS;

ESCHERICHIA COLI;

ESCHERICHIA COLI; PICHIA; PICHIA PASTORIS;

EID: 42549090967     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11274-007-9554-9     Document Type: Article

References (30)

1. Boado RJ, Ji A, Pardridge WM (2000) Cloning and expression in Pichia pastoris of a genetically engineered single chain antibody against the rat transferrin receptor. J Drug Target 8:403-412
2. Brichta J, Vesela H, Franek M (2003) Production of scFv recombinant fragments against 2,4-dichlorophenoxyacetic acid hapten using naïve phage library. Vet Med 48:237-247
3. Brierley RA, Bussineau C, Kosson R et al (1990) Fermentation development of recombinant Pichia pastoris expressing the heterologous gene: bovine lysozyme. Ann N Y Acad Sci 589:350-362
4. Burrowes OJ, Diamond G, Lee TC (2005) Recombinant expression of pleurocidin cDNA using the Pichia pastoris expression system. J Biomed Biotechnol 4:374-384
5. Clare JJ, Romanos MA (1995) Expression of cloned genes in the yeasts Saccharomyces cerevisiae and Pichia pastoris. Methods Mol Cell Biol 5:319-329
6. Couderc R, Baratti J (1980) Oxidation of methanol by the yeast, Pichia pastoris. Purification and properties of the alcohol oxidase. Agric Biol Chem 44: 2279-2289
7. Cregg JM, Higgins DR (1995) Production of foreign proteins in the yeast Pichia pastoris. Can J Bot 73:91-97
8. Cregg JM, Lin-Cereghino J, Shi J et al (2000) Recombinant protein expression in Pichia pastoris. Mol Biotechnol 16:23-52
9. Cregg JM, Madden KR (1988) Development of the methylotrophic yeast, Pichia pastoris, as a host system for the production of foreign proteins. In: Pierce G (ed) Development in industrial microbiology, vol 29. Elsevier Science, Amsterdam, pp 33-41
10. Cregg JM, Vedvick TS, Raschke WC (1993) Recent advances in the expression of foreign genes in Pichia pastoris. Bio/Technology 11:905-910
11. Eldin P, Pauza ME, Hieda Y et al (1997) High-level secretion of two antibody single chain Fv fragments by Pichia pastoris. J Immunol Methods 201:67-75
12. Fischer R, Drossard J, Emans N et al (1999) Towards molecular farming in the future: Pichia pastoris-based production of single-chain antibody fragments. Biotechnol Appl Biochem 30:117-120
13. Franek M, Hruska K (2005) Antibody based methods for environmental and food analysis. Vet Med 50:1-10
14. Freyre FM, Vazquez JE, Ayala M et al (2000) Very high expression of an anti-carcinoembryonic antigen single chain Fv antibody fragment in the yeast Pichia pastoris. J Biotechnol 76:157-163
15. Gellissen G, Kunze G, Gaillardin C et al (2005) New yeast expression platforms based on methylotrophic Hansenula polymorpha and Pichia pastoris and dimorphic Arxula adeninivorans and Yarrowia lipolytica-A comparison. FEMS Yeast Res 5:1079-1096
16. Gurkan C, Symeonides SN, Ellar DJ (2004) High-level production in Pichia pastoris of an anti-p185HER-2 scFv using an alternative secretion expression vector. Biotechnol Appl Biochem 39:115-122
17. Hellwig S, Emde F, Raven NPG et al (2001) Analysis of. single-chain antibody production in Pichia pastoris using on-line methanol control in fed-batch and mixed-feed fermentations. Biotechnol Bioeng 74:344-352
18. Higgins DR, Cregg JM (1998) Methods in molecular biology. In: Pichia Protocols. Humana Press, New Jersey, pp 1-16
19. Lange S, Schmitt J, Schmid RD (2001) High-level expression of the recombinant, atrazine-specific Fab fragment K411B by the methylotrophic yeast Pichia pastoris. J Immunol Methods 255:103-114
20. Li T, Zhang Q, Liu Y et al (2006) Production of recombinant ScFv antibodies against methamidophos from a phage-display library of a hyperimmunized mouse. J Agric Food Chem 54:9085-9091
21. Liu FQ, Xu ZG, Wang JS (1998) The development of an ELISA for quantitation of methamidophos and its application. J Agric Biotech 6:140-144
22. Miller KD, Weaver-Feldhaus J, Gray SA et al (2005) Production, purification, and characterization of human scFv antibodies expressed in Saccharomyces cerevisiae, Pichia pastoris, and Escherichia coli. Protein Expr Purif 42:255-267
23. Neri D, Montigiani S, Kirkham PM (1996) Biophysical methods for the determination of antibody-antigen affinities. Trends Biotechnol 14:465-470
24. Ohtani W, Nawa Y, Takeshima K et al (1998) Physicochemical and immunochemical properties of recombinant human serum albumin from Pichia pastoris. Anal Biochem 256:56-62
25. Shi X, Karkut T, Chamankhah M et al (2003) Optimal conditions for the expression of a single-chain antibody (scFv) gene in Pichia pastoris. Protein Expr Purifi 28:321-330
26. Smith JA (1992) Bradford method. In: Ausubel FM, Bent R, Kingston RE (eds) Short protocols in molecular biology. John Wiley and Sons, Boston, pp 336-337
27. Sreekrishna K, Brankamp RG, Kropp KE et al (1997) Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris. Gene 190:55-62
28. Wang YC, Chen ZR, Chen ZW et al (2002) Recombinant human heparin-binding neurite-promoting factor expressed with yeast stimulates neuritis out growth. Chin Med J 115:1352-1357
29. Zhang YD, Yang BL (2006) In vivo optimizing of intracellular production of heterologous protein in Pichia pastoris by fluorescent scanning. Anal Biochem 357:232-239
30. Zhao SQ, Sun YM, Zhang CY et al (2003) Studies on purification of methamidophos monoclonal antibodies and comparative immunoactivity of purified antibodies. Biomed Environ Sci 16:119-125