Development of a multiplex UPLC-MRM MS method for quantification of human membrane transport proteins OATP1B1, OATP1B3 and OATP2B1 in in vitro systems and tissues

Analytica Chimica Acta

Volumn 717, Issue , 2012, Pages 67-76

  Ji, Chengjie ^a   Tschantz, William R ^a   Pfeifer, Nathan D ^a   Ullah, Mohammed ^a   Sadagopan, Nalini ^a  

^a PFIZER INC   (United States)

Author keywords

Organic anion transporting polypeptides (OATP1B1, OATP1B3 and OATP2B1); Purified membrane protein standards; Quantification; UPLC MRM MS

Indexed keywords

ABSOLUTE QUANTIFICATION; CALIBRATION CURVES; COEFFICIENT OF VARIATION; EXPRESSION LEVELS; HUMAN BRAIN CAPILLARY; HUMAN HEPATOCYTES; IN-VITRO; MEMBRANE FRACTION; MEMBRANE PROTEINS; MEMBRANE TRANSPORT PROTEINS; MOUSE LIVER; ORGANIC ANION TRANSPORTING POLYPEPTIDES (OATP1B1, OATP1B3 AND OATP2B1); ORGANIC ANIONS; PLASMID VECTORS; QUANTIFICATION; QUANTIFICATION METHODS; SCALE-UP; SIMULTANEOUS DETERMINATIONS; SLCO1B1; TISSUE SAMPLES; TRYPTIC DIGESTS; UPLC-MRM MS; VITRO-IN VIVO CORRELATION; WILD TYPES;

BIOCHEMISTRY; BIOLOGICAL MEMBRANES; ENDOTHELIAL CELLS; MAMMALS; MEMBRANES; PURIFICATION; QUALITY CONTROL; STANDARDS; TISSUE;

PROTEINS;

CHEMICAL COMPOUND; ENDOBIOTICS; MEMBRANE PROTEIN; ORGANIC ANION TRANSPORTER; ORGANIC ANION TRANSPORTER 1B1; ORGANIC ANION TRANSPORTER 1B3; ORGANIC ANION TRANSPORTER 2; ORGANIC ANION TRANSPORTER 2B1; PLASMID VECTOR; POLYPEPTIDE; UNCLASSIFIED DRUG; XENOBIOTIC AGENT;

ACCURACY; ANIMAL CELL; ARTICLE; BRAIN CAPILLARY; CALIBRATION; CAPILLARY ENDOTHELIUM; CELL LINE; CONTROLLED STUDY; DRUG DISPOSITION; ENDOTHELIUM CELL; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; LIVER; LIVER CELL; LIVER CLEARANCE; MASS SPECTROMETRY; MOUSE; MULTIPLE REACTION MONITORING MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; QUALITY CONTROL; SCALE UP; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY;

ANIMALS; BRAIN; CAPILLARIES; CELL LINE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ENDOTHELIAL CELLS; HEPATOCYTES; HUMANS; MASS SPECTROMETRY; MICE; ORGANIC ANION TRANSPORTERS; ORGANIC ANION TRANSPORTERS, SODIUM-INDEPENDENT; SENSITIVITY AND SPECIFICITY; TRANSFECTION;

EID: 84856492304     PISSN: 00032670     EISSN: 18734324     Source Type: Journal    
DOI: 10.1016/j.aca.2011.12.005     Document Type: Article

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