The transition of human estrogen sulfotransferase from generalist to specialist using directed enzyme evolution

Journal of Molecular Biology

Volumn 416, Issue 1, 2012, Pages 21-32

  Amar, Dotan ^a   Berger, Ilana ^a   Amara, Neri ^a   Tafa, Gemechu ^a   Meijler, Michael M ^a   Aharoni, Amir ^a  

^a BEN GURION UNIVERSITY OF THE NEGEV   (Israel)

Author keywords

broad specificity; cytosolic sulfotransferases; detoxification; directed evolution; thermostability

Indexed keywords

COUMARIN; ESTROGEN SULFOTRANSFERASE; MUTANT PROTEIN; PHENOL; SULFATE;

ARTICLE; BINDING SITE; CONTROLLED STUDY; ENZYMATIC ASSAY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ISOLATION; ENZYME SPECIFICITY; GENE MUTATION; HUMAN; ION TRANSPORT; MOLECULAR EVOLUTION; NONHUMAN; PRIORITY JOURNAL; SCREENING; THERMOSTABILITY; WILD TYPE;

EID: 84856432368     PISSN: 00222836     EISSN: 10898638     Source Type: Journal    
DOI: 10.1016/j.jmb.2011.12.013     Document Type: Article

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