A metabolomic strategy defines the regulation of lipid content and global metabolism by Δ9 desaturases in Caenorhabditis elegans

BMC Genomics

Volumn 13, Issue 1, 2012, Pages

  Castro, Cecilia ^a,b   Sar, Funda ^a,c   Shaw, W Robert ^a,c   Mishima, Masanori ^a,c   Miska, Eric A ^a,c   Griffin, Julian L ^a,b,d  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^c UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^d MEDICAL RESEARCH COUNCIL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ACYL COENZYME A DESATURASE; CHOLESTEROL ESTER; PHOSPHOLIPID; TRIACYLGLYCEROL; FATTY ACID; LIPID;

AMINO ACID METABOLISM; ARTICLE; CAENORHABDITIS ELEGANS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME METABOLISM; ENZYME REGULATION; FAT 5 GENE; FAT 6 GENE; FAT 7 GENE; FATTY ACID ANALYSIS; FATTY ACID SYNTHESIS; GENE; GENE EXPRESSION; GENE FUNCTION; GENE INACTIVATION; GENE MUTATION; LIPID ANALYSIS; LIPID COMPOSITION; LIPID METABOLISM; LIPOGENESIS; LIQUID CHROMATOGRAPHY; MASS FRAGMENTOGRAPHY; MASS SPECTROMETRY; METABOLOMICS; MUTANT; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PROTEIN EXPRESSION; WILD TYPE; ANIMAL; CHEMISTRY; DRUG ANTAGONISM; ENZYMOLOGY; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; METABOLISM; METABOLOME;

CAENORHABDITIS ELEGANS;

ANIMALS; CAENORHABDITIS ELEGANS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; FATTY ACIDS; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; GENE KNOCKOUT TECHNIQUES; LIPIDS; MAGNETIC RESONANCE SPECTROSCOPY; MASS SPECTROMETRY; METABOLOME; STEAROYL-COA DESATURASE;

EID: 84855986168     PISSN: None     EISSN: 14712164     Source Type: Journal    
DOI: 10.1186/1471-2164-13-36     Document Type: Article

References (38)

1. Spiegelman BM, Flier JS. Obesity and the regulation of energy balance. Cell 2001, 104:531-43. 10.1016/S0092-8674(01)00240-9, 11239410.
2. Lewis GF, Carpentier A, Adeli K, Giacca A. Disordered fat storage and mobilization in the pathogenesis of insulin resistance and type 2 diabetes. Endocr Rev 2002, 23:201-229. 10.1210/er.23.2.201, 11943743.
3. Kopelman PG. Obesity as a medical problem. Nature 2000, 404:635-643.
4. Allison DB, Neale MC, Kezis MI, Alfonso VC, Heshka S, Heymsfield SB. Assortative mating for relative weight: genetic implications. Behav Genet 1996, 26:103-111. 10.1007/BF02359888, 8639146.
5. Friedman JM. A war on obesity, not the obese. Science 2003, 299:856-858. 10.1126/science.1079856, 12574619.
6. Stunkard AJ, Harris JR, Pedersen NL, McClearn GE. The body-mass index of twins who have been reared apart. N Engl J Med 1990, 322:1483-7. 10.1056/NEJM199005243222102, 2336075.
7. Despres JP, Moorjani S, Lupien PJ, Tremblay A, Nadeau A, Bouchard C. Genetic aspects of susceptibility to obesity and related dyslipidemias. Mol Cell Biochem 1992, 113:151-169.
8. Brockmann GA, Bevova MR. Using mouse models to dissect the genetics of obesity. Trends Genet 2002, 18:367-76. 10.1016/S0168-9525(02)02703-8, 12127777.
9. Delrue MA, Michaud JL. Fat chance: genetic syndromes with obesity. Clin Genet 2004, 66:83-93. 10.1111/j.0009-9163.2004.00300.x, 15253756.
10. Ashrafi K. Obesity and the regulation of fat metabolism. Worm Book 2007, 1-20. The C. elegans Research Community.
11. Nomura T, Horikawa M, Shimamura S, Hashimoto T, Sakamoto K. Fat accumulation in Caenorhabditis elegans is mediated by SREBP homolog SBP-1. Genes Nutr 2010, 5:17-27. 10.1007/s12263-009-0157-y, 2820192, 19936816.
12. Ashrafi K, Chang FY, Watts JL, Fraser AG, Kamath RS, Ahringer J, Ruvkun G. Genome-wide RNAi analysis of Caenorhabditis elegans fat regulatory genes. Nature 2003, 421:268-72. 10.1038/nature01279, 12529643.
13. McKay RM, McKay JP, Avery L, Graff JM. C elegans: a model for exploring the genetics of fat storage. Dev Cell 2003, 4:131-142. 10.1016/S1534-5807(02)00411-2, 12530969.
14. Suh JM, Zeve D, McKay R, Seo J, Salo Z, Li R, Wang M, Graff JM. Adipose is a conserved dosage-sensitive antiobesity gene. Cell Metab 2007, 6:195-207. 10.1016/j.cmet.2007.08.001, 2587167, 17767906.
15. Watts JL, Browse J. Genetic dissection of polyunsaturated fatty acid synthesis in Caenorhabditis elegans. Proc Natl Acad Sci USA 2002, 99:5854-9. 10.1073/pnas.092064799, 122866, 11972048.
16. Nakamura MT, Nara TY. Structure, function, and dietary regulation of Δ6, Δ5, and Δ9 desaturases. Annu Rev Nutr 2004, 24:345-376. 10.1146/annurev.nutr.24.121803.063211, 15189125.
17. Ntambi JM, Miyazaki M. Regulation of stearoyl-CoA desaturases and role in metabolism. Prog Lipid Res 2004, 43:91-104. 10.1016/S0163-7827(03)00039-0, 14654089.
18. Brock TJ, Browse J, Watts JL. Genetic regulation of unsaturated fatty acid composition in C elegans. PLoS Genet 2006, 2:997-1005.
19. Brock TJ, Browse J, Watts JL. Fatty acid desaturation and the regulation of adiposity in Caenorhabditis elegans. Genetics 2007, 176:865-875. 1894614, 17435249.
20. Liang B, Ferguson K, Kadyk L, Watts JL. The role of nuclear receptor NHR-64 in fat storage regulation in Caenorhabditis elegans. PLoS One 2010, 5:e9869. 10.1371/journal.pone.0009869, 2845610, 20360843.
21. Soukas AA, Kane EA, Carr CE, Melo JA, Ruvkun G. Rictor/TORC2 regulates fat metabolism, feeding, growth, and life span in Caenorhabditis elegans. Gene Dev 2009, 23:496-511. 10.1101/gad.1775409, 2648650, 19240135.
22. O'Rourke EJ, Soukas AA, Carr CE, Ruvkun G. C. elegans major fats are stored in vesicles distinct from lysosome-related Organelles. Cell Metab 2009, 10:430-435. 10.1016/j.cmet.2009.10.002, 2921818, 19883620.
23. Yen K, Le TT, Bansal A, Narasimhan SD, Cheng JX, Tissenbaum HA. A comparative study of fat storage quantitation in nematode Caenorhabditis elegans using label and label-free methods. PLoS One 2010, 5:e12810.
24. Brooks KK, Liang B, Watts JL. The influence of bacterial diet on fat storage in C. elegans. PLoS One 2009, 4:e7545. 10.1371/journal.pone.0007545, 2760100, 19844570.
25. Badawy AA, Morgan CJ, Turner JA. Application of the Phenomenex EZ:faast™ amino acid analysis kit for rapid gas-chromatographic determination of concentrations of plasma tryptophan and its brain uptake competitors. Amino Acids 2008, 34:587-596. 10.1007/s00726-007-0012-7, 2797848, 18071842.
26. Kniazeva M, Crawford QT, Seiber M, Wang C, Han M. Monomethyl branched -chain fatty acids play an essential role in Caenorhabditis elegans development. PLoS Biol 2004, 2:1446-1459.
27. Le TT, Duren HM, Slipchenko MN, Hu CD, Cheng JX. Label-free quantitative analysis of lipid metabolism in living Caenorhabditis elegans. J Lipid Res 2010, 51:672-677. 10.1194/jlr.D000638, 2817597, 19776402.
28. Dobrzyn A, Ntambi JM. The role of stearoyl-CoA desaturase in the control of metabolism. Prostaglandins Leukot Essent Fatty Acids 2005, 73:35-41. 10.1016/j.plefa.2005.04.011, 15941655.
29. van Gilst MR, Hadjivassiliou H, Yamamoto KR. A Caenorhabditis elegans nutrient response system partially dependent on nuclear receptor NHR-49. Proc Natl Acad Sci USA 2005, 102:13496-501. 10.1073/pnas.0506234102, 1201344, 16157872.
30. Horikawa M, Sakamoto K. Fatty acid metabolism is involved in stress-resistance mechanisms of Caenorhabditis elegans. Biochem Biophys Res Commun 2009, 390:1402-1407. 10.1016/j.bbrc.2009.11.006, 19896458.
31. Zhang SO, Box AC, Xu N, Le Men J, Yu J, Guo F, Trimble R, Mak HY. Genetic and dietery regulation of lipid droplet expansion in Caenorhabditis elegans. Proc Natl Acad Sci USA 2010, 107:4640-4645. 10.1073/pnas.0912308107, 2842062, 20176933.
32. Boyer HW, Roulland-Dussoix D. A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol 1969, 41:459-72. 10.1016/0022-2836(69)90288-5, 4896022.
33. Brenner S. The genetics of Caenorhabditis elegans. Genetics 1974, 77:71-94. 1213120, 4366476.
34. Atherton HJ, Jones OAH, Malik S, Miska EA, Griffin JL. A comparative metabolomic study of NHR-49 in Caenorhabditis elegans and PPAR-α in the mouse. FEBS Lett 2008, 582:1661-1666. 10.1016/j.febslet.2008.04.020, 18435929.
35. Fuchs S, Bundy JG, Davies SK, Viney JM, Swire JS, Leroi AM. A metabolic signature of long life in Caenorhabditis elegans. BMC Biol 2010, 8:14. 10.1186/1741-7007-8-14, 2829508, 20146810.
36. Blaise BJ, Giacomotto J, Elena B, Dumas ME, Toulhot P, Segalat L, Emsley L. Metabotyping of Caenorhabditis elegans reveals latent phenotypes. Proc Natl Acad Sci USA 2007, 104:19808-19812. 10.1073/pnas.0707393104, 2148380, 18077412.
37. van Velzen EJJ, Westerhuis JA, van Duynhoven JPM, van Dorsten FA, Hoefsloot HCJ, Jacobs DM, Smit S, Draijer R, Kroner CI, Smilde AK. Multilevel data analysis of a crossover designed human nutritional intervention study. J Proteome Res 2008, 7:4483-4491. 10.1021/pr800145j, 18754629.
38. Schäfer J, Strimmer K. A shrinkage approach to large-scale covariance matrix estimation and implications for functional genomics. Stat Appl Genet Mol Biol 2005, 4:32.