Differential expression and biological activity of two piscidin paralogues and a novel splice variant in Atlantic cod (Gadus morhua L.)

Fish and Shellfish Immunology

Volumn 32, Issue 3, 2012, Pages 396-406

  Ruangsri, Jareeporn ^a   Salger, Scott A ^b   Caipang, Christopher M A ^a   Kiron, Viswanath ^a   Fernandes, Jorge M O ^a  

^a NORD UNIVERSITY   (Norway)

^b NORTH CAROLINA STATE UNIVERSITY   (United States)

Author keywords

Antimicrobial peptide; Gadus morhua; Innate immunity; Piscidin; Positive selection

Indexed keywords

BACTERIA (MICROORGANISMS); GADUS MORHUA; LISTONELLA ANGUILLARUM; TETRAHYMENA PYRIFORMIS; YERSINIA RUCKERI;

EID: 84855958149     PISSN: 10504648     EISSN: 10959947     Source Type: Journal    
DOI: 10.1016/j.fsi.2011.11.022     Document Type: Article

References (43)

1. Zasloff M. Innate immunity, antimicrobial peptides, and protection of the oral cavity. The Lancet 2002, 360:1116-1117.
2. Noga E.J., Silphaduang U., Park N.G., Seo J.K., Stephenson J., Kozlowicz S. Piscidin 4, a novel member of the piscidin family of antimicrobial peptides. Comp Biochem Physiol B 2009, 152:299-305.
3. Lee S.A., Kim Y.K., Lim S.S., Zhu W.L., Ko H., Shin S.Y., et al. Solution structure and cell selectivity of piscidin 1 and its analogues. Biochemistry 2007, 46:3653-3663.
4. Smith V.J., Fernandes J.M.O. Antimicrobial peptides of the innate immune system. Fish defenses 2008, 241-276. Science Publishers, USA. G. Zaccone, J.J. Marchalonis, S.F. Schluter, J. Meseguer, B.G. Kapoor (Eds.).
5. Silphaduang U., Noga E.J. Peptide antibiotics in mast cells of fish. Nature 2001, 414:268-269.
6. Lauth X., Shike H., Burns J.C., Westerman M.E., Ostland V.E., Carlberg J.M., et al. Discovery and characterization of two isoforms of moronecidin, a novel antimicrobial peptide from hybrid striped bass. JBiol Chem 2002, 277:5030-5039.
7. Salerno G., Parrinello N., Roch P., Cammarata M. cDNA sequence and tissue expression of an antimicrobial peptide, dicentracin; a new component of the moronecidin family isolated from head kidney leukocytes of sea bass, Dicentrarchus labrax. Comp Biochem Physiol B 2007, 146:521-529.
8. Iijima N., Tanimoto N., Emoto Y., Morita Y., Uematsu K., Murakami T., et al. Purification and characterization of three isoforms of chrysophsin, a novel antimicrobial peptide in the gills of the red sea bream, Chrysophrys major. Eur J Biochem 2003, 270:675-686.
9. Sun B.J., Xie H.X., Song Y., Nie P. Gene structure of an antimicrobial peptide from mandarin fish, Siniperca chuatsi (Basilewsky), suggests that moronecidins and pleurocidins belong in one family: the piscidins. JFish Dis 2007, 30:335-343.
10. Chen Y., Xu B., Su Y. The amplification and sequence analysis of piscidin-like gene from two marine fish Epinephelus akaara and Pseudosciaena crocea. Biotechnology 2008, 136. S545-S54S.
11. Yin Z.X., He W., Chen W.J., Yan J.H., Yang J.N., Chan S.M., et al. Cloning, expression and antimicrobial activity of an antimicrobial peptide, epinecidin-1, from the orange-spotted grouper, Epinephelus coioides. Aquaculture 2006, 253:204-211.
12. Cole A.M., Weis P., Diamond G. Isolation and characterization of pleurocidin, an antimicrobial peptide in the skin secretions of winter flounder. JBiol Chem 1997, 272:12008-12013.
13. Patrzykat A., Gallant J.W., Seo J.K., Pytyck J., Douglas S.E. Novel antimicrobial peptides derived from flatfish genes. Antimicrob Agents Chemother 2003, 47:2464-2470.
14. Silphaduang U., Colorni A., Noga E.J. Evidence for widespread distribution of piscidin antimicrobial peptides in teleost fish. Dis Aquat Org 2006, 72:241-252.
15. Mulero I., Noga E.J., Meseguer J., Garcia-Ayala A., Mulero V. The antimicrobial peptides piscidins are stored in the granules of professional phagocytic granulocytes of fish and are delivered to the bacteria-containing phagosome upon phagocytosis. Dev Comp Immunol 2008, 32:1531-1538.
16. Chinchar V.G., Bryan L., Silphadaung U., Noga E., Wade D., Rollins-Smith L. Inactivation of viruses infecting ectothermic animals by amphibian and piscine antimicrobial peptides. Virology 2004, 323:268-275.
17. Sung W.S., Lee J., Lee D.G. Fungicidal effect of piscidin on Candida albicans: pore formation in lipid vesicles and activity in fungal membranes. Biol Pharm Bull 2008, 31:1906-1910.
18. Colorni A., Ullal A., Heinisch G., Noga E.J. Activity of the antimicrobial polypeptide piscidin 2 against fish ectoparasites. JFish Dis 2008, 31:423-432.
19. Pilström L., Warr G.W., Strömberg S. Why is the antibody response of Atlantic cod so poor? The search for a genetic explanation. Fish Sci 2005, 71:961-971.
20. Ruangsri J., Fernandes J.M.O., Brinchmann M., Kiron V. Antimicrobial activity in the tissues of Atlantic cod (Gadus morhua L.). Fish Shellfish Immunol 2010, 28:879-886.
21. Fernandes J., Ruangsri J., Kiron V. Atlantic cod piscidin and its diversification through positive selection. PLoS ONE 2010, 5:e9501.
22. Browne M.J., Feng C.Y., Booth V., Rise M.L. Characterization and expression studies of Gaduscidin-1 and Gaduscidin-2; paralogous antimicrobial peptide-like transcripts from Atlantic cod (Gadus morhua). Dev Comp Immunol 2011, 35:399-408.
23. Kiefer F., Arnold K., Künzli M., Bordoli L., Schwede T. The SWISS-MODEL repository and associated resources. Nucl Acids Res 2009, 37:D387-D392.
24. Noga E.J., Ullal A.J., Corrales J., Fernandes J.M.O. Application of antimicrobial polypeptide host defenses to aquaculture: exploitation of downregulation and upregulation responses. Comp Biochem Physiol D 2011, 6:44-54.
25. Edgar R.C. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 2004, 32:1792-1797.
26. Hall T.A. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp 1999, 95-98.
27. Guindon S., Gascuel O. Asimple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. JSyst Biol 2003, 52:696-704.
28. Ronquist F., Huelsenbeck J.P. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 2003, 19:1572-1574.
29. Fernandes J.M., Macqueen D.J., Lee H.T., Johnston I.A. Genomic, evolutionary, and expression analyses of cee, an ancient gene involved in normal growth and development. Genomics 2008, 91:315-325.
30. Fernandes J.M., Mommens M., Hagen O., Babiak I., Solberg C. Selection of suitable reference genes for real-time PCR studies of Atlantic halibut development. Comp Biochem Physiol B 2008, 150:23-32.
31. Fernandes J.M., Mackenzie M.G., Wright P.A., Steele S.L., Suzuki Y., Kinghorn J.R., et al. Myogenin in model pufferfish species: comparative genomic analysis and thermal plasticity of expression during early development. Comp Biochem Physiol D 2006, 1:35-45.
32. Vandesompele J., De Preter K., Pattyn F., Poppe B., Van Roy N., De Paepe A., et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 2002, 3:1-11. Research 0034.
33. Fernandes J.M., Saint N., Kemp G.D., Oncorhyncin Smith VJ. a potent antimicrobial peptide derived from the non-histone chromosomal protein H6 of rainbow trout, Oncorhynchus mykiss. Biochem J 2003, 373:621-628.
34. Fernandes J.M., Kemp G.D., Molle M.G., Smith V.J. Anti-microbial properties of histone H2A from skin secretions of rainbow trout, Oncorhynchus mykiss. Biochem J 2002, 368:611-620.
35. Ullal A.J., Litaker R.W., Noga E.J. Antimicrobial peptides derived from hemoglobin are expressed in epithelium of channel catfish (Ictalurus punctatus, Rafinesque). Dev Comp Immunol 2008, 32:1301-1312.
36. Lewis B.P., Green R.E., Brenner S.E. Evidence for the widespread coupling of alternative splicing and nonsense-mediated mRNA decay in humans. PNatl Acad Sci USA 2003, 100:189-192.
37. Fernandes J.M.O., MacKenzie M.G., Kinghorn J.R., Johnston I.A. FoxK1 splice variants show developmental stage-specific plasticity of expression with temperature in the tiger pufferfish. JExp Biol 2007, 210:3461-3472.
38. Fernandes J.M., Kinghorn J.R., Johnston I.A. Differential regulation of multiple alternatively spliced transcripts of MyoD. Gene 2007, 391:178-185.
39. Yeaman M.R., Yount N.Y. Mechanisms of antimicrobial peptide action and resistance. Pharmacol Rev 2003, 55:27-55.
40. Silhavy T.J., Kahne D., Walker S. The bacterial cell envelope. Cold Spring Harb Perspect Biol 2010, 2:a000414.
41. Park N.G., Silphaduang U., Moon H.S., Seo J.K., Corrales J., Noga E.J. Structure-activity relationships of piscidin 4, a piscine antimicrobial peptide. Biochemistry 2011, 50:3288-3299.
42. Seppola M., Johnsen H., Mennen S., Myrnes B., Tveiten H. Maternal transfer and transcriptional onset of immune genes during ontogenesis in Atlantic cod. Dev Comp Immunol 2009, 33:1205-1211.
43. Lilleeng E., Frøystad M.K., Vekterud K., Valen E.C., Krogdahl å Comparison of intestinal gene expression in Atlantic cod (Gadus morhua) fed standard fish meal or soybean meal by means of suppression subtractive hybridization and real-time PCR. Aquaculture 2007, 267:269-283.