Purification and characterization of three isoforms of chrysophsin, a novel antimicrobial peptide in the gills of the red sea bream, Chrysophrys major

European Journal of Biochemistry

Volumn 270, Issue 4, 2003, Pages 675-686

  Iijima, Noriaki ^a   Tanimoto, Norio ^a   Emoto, Yohko ^a   Morita, Yohko ^a   Uematsu, Kazumasa ^a   Murakami, Tomoya ^b   Nakai, Toshihiro ^a  

^a HIROSHIMA UNIVERSITY   (Japan)

^b Hiroshima Fisheries Experimental Station   (Japan)

Author keywords

Antimicrobial peptide; Chrysophsin; Gills; Red sea bream; Synthetic peptide

Indexed keywords

CHRYSOPHSIN 1; CHRYSOPHSIN 2; CHRYSOPHSIN 3; FISH PROTEIN; ISOPROTEIN; SYNTHETIC PEPTIDE; UNCLASSIFIED DRUG;

ALPHA HELIX; AMINO ACID SEQUENCE; ANIMAL TISSUE; ARTICLE; BACILLUS SUBTILIS; BACTERICIDAL ACTIVITY; CARBOXY TERMINAL SEQUENCE; CIRCULAR DICHROISM; CRUSTACEA; ESCHERICHIA COLI; GILL; GRAM NEGATIVE BACTERIUM; HEMOLYSIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; IMMUNOHISTOCHEMISTRY; MASS SPECTROMETRY; NONHUMAN; PREDICTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN FUNCTION; PROTEIN ISOLATION; PROTEIN LOCALIZATION; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; SEA BREAM; TISSUE DISTRIBUTION;

AMINO ACID SEQUENCE; ANIMALS; ANTI-BACTERIAL AGENTS; ANTIMICROBIAL CATIONIC PEPTIDES; BACTERIA; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CIRCULAR DICHROISM; GILLS; HEMOLYSIS; HUMANS; IMMUNOENZYME TECHNIQUES; MASS SPECTROMETRY; MELITTEN; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; PROTEIN ISOFORMS; PROTEIN TRANSPORT; SEA BREAM; SEQUENCE HOMOLOGY, AMINO ACID; XENOPUS LAEVIS; XENOPUS PROTEINS;

ANIMALIA; ARCHOSARGUS RHOMBOIDALIS; BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); CHRYSOPHRYS; CRUSTACEA; ESCHERICHIA COLI; HYPEROGLYPHE POROSA; NEGIBACTERIA; PAGRUS MAJOR; POSIBACTERIA;

EID: 0037293964     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1033.2003.03419.x     Document Type: Article

References (37)

1. Zasloff, M. (2002) Antimicrobial peptides of multicellular organisms. Nature (London) 415, 389-395.
2. Hancock, R.E. & Scott, M.G. (2000) The role of antimicrobial peptides in animal defenses. Proc. Natl. Acad. Sci. USA 97, 8856-8861.
3. Hancock, R.E. & Diamond, G. (2000) The role of cationic antimicrobial peptides in innate host defences. Trends Microbiol. 8, 402-410.
4. Evelyn, T.P.T. (1996) Infection and disease. In The Fish Immune System: Organism, Pathogen, and Environment (Iwama, G. & Nakanishi, T., eds), pp. 339-366. Academic Press, Inc, San Diego.
5. Yano, T. (1996) The nonspecific immune system: humoral defense. In The Fish Immune System: Organism, Pathogen, and Environment (Iwama, G. & Nakanishi, T., eds), pp. 105-157. Academic Press, Inc, San diego.
6. Lemaitre, C., Orange, N., Saglio, P., saint, N., Gagnon, J. & Molle, G. (1996) Characterization and ion channel activities of novel antibacterial proteins from skin mucosa of carp (Cyprinus carpio). Eur. J. Biochem. 240, 143-149.
7. Robinette, D., Wada, S., Arroll, T., Levy, M.G., Miller, W.L. & Noga, E.J. (1998) Antimicrobial activity in the skin of the channel catfish Ictalurus punctatus: characterization of broad-spectrum histone-like antimicrobial proteins. CMLS, Cell. Mol. Life Sci. 54, 467-475.
8. Tossi, A., Sandri, L. & Giangaspero, A. (2000) Amphipathic, alpha-helical antimicrobial peptides. Biopolymers 55, 4-30.
9. Robinette, D.W. & Noga, E.J. (2001) Histone-like protein: a novel method for measuring stress in fish. Diseases of Aquatic Organisms 44, 97-107.
10. Richards, R.C., O'Neil, D.B., Thibault, P. & Ewart, K.V. (2001) Histone H1: an antimicrobial protein of Atlantic salmon (Salmo salar). Biochem. Biophys. Res. Commun. 284, 549-555.
11. Oren, Z. & Shai, Y. (1996) A class of highly potent antibacterial peptides derived from pardaxin, a pore-forming peptide isolated from moses sole fish Pardachirus marmoratus. Eur. J. Biochem. 237, 303-310.
12. Cole, A.M., Weis, P. & Diamond, G. (1997) Isolation and characterization of pleurocidin, an antimicrobial peptide in the skin secretions of winter flounder. J. Biol. Chem. 272, 12008-12013.
13. Park, I.Y., Park, C.B., Kim, M.S. & Kim, S.C. (1998) Parasin I, an antimicrobial peptide derived from histone H2A in the catfish, Parasiluras asotus. FEBS Lett. 437, 258-262.
14. Douglas, S.E., Gallant, J.W., Gong, Z. & Hew, C. (2001) Cloning and developmental expression of a family of pleurocidin-like antimicrobial peptides from winter flounder, Pleuronectes americanus (Walbaum). Dev. Comp. Immunol. 25, 137-147.
15. Park, C.B., Lee, J.H., Park, I.Y., Kim, M.S. & Kim, S.C. (1997) A novel antimicrobial peptide from the loach, Misgurnus anguillicaudatus. FEBS Lett. 411, 173-178.
16. Silphaduang, U. & Noga, E.G. (2001) Peptide antibiotics in mast cells of fish. Nature (London) 414, 268-269.
17. Lauth, X Shike, H., Burns, J.C., Westerman, M.E., Ostland, V.E., Carlberg, J.M., Van Olst, J.C., Nizet, V., Taylor, S.W., Shimizu, C. & Bulet, P. (2002) Discovery and characterization of two isoforms of moronecidin, a novel antimicrobial peptide from hybrid striped bass. J. Biol. Chem. 277, 5030-5039.
18. Shike, H., Lauth, X., Westerman, M.E., Ostland, V.E., Carlberg, J.M., Van Olst, J.C., Shimizu, C., Bulet, P. & Burns, J.C. (2002) Bass hepcidin is a novel antimicrobial peptide induced by bacterial challenge. Eur. J. Biochem. 269, 2232-2237.
19. Zeng, R. Xu, Q., Shao, X.-X., Wang, K.-Y. & Xia Q.-C. (1999) Characterization and analysis of a novel glycoprotein from snake venom using liquid chromatography-electrospray mass spectrometry and edman degradation. Eur. J. Biochem. 266, 352-358.
20. Fields, G.B. & Noble, R.L. (1990) Solid phase peptide synthesis utilizing 9-fluorenylmethoxycarbonyl amino acids. Int. J. Pept. Protein Res. 35, 161-214.
21. Noda, M., Yamaguchi, M., Ando, E., Takeda, K. & Nokihara, K. (1994) Synthesis of 5-{[(R,S)-5-[(9-fluorenylmethoxycarbonyl) amino]-10,11-dihydrobenzo[alfa,d]cyclopenten-2-yl]valeric acid (CHA) and 5-{[(R,S)-5-[(9-fluorenylmethoxycarbonyl) amino] dibenzo[alfa,d]cyclohepten-2-yl]valeric acid (CHA) handles for the solid-phase synthesis of C-terminal peptide amides under mild conditions. J. Org. Chem. 59, 7965-7975.
22. Matsuzaki, K. (1998) Magainins as paradigm for the mode of action of pore forming polypeptides. Biochim. Biophy. Acta 1376, 391-400.
23. Schagger, H. & von Jagow, G. (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166, 368-379.
24. Gill, S.C. & von Hippel, P.H. (1989) Caluculation of protein extinction coefficients from amino acid sequence data. Anal. Biochem. 182, 319-326.
25. Juban, M.M., Javadpour, M.M. & Barkley, M.D. (1997) Circular dichroism studies of secondary structure of peptides. In Antibacterial Peptide Protocols (Shafer, W.M., ed.), pp. 73-84. Humanna Press, Totowa, NJ.
26. McLean, L.R., Hagaman, K.A., Owen, T.J. & Krstenansky, J.L. (1991) Minimal peptide length for interaction of amphipathic α-helical peptides with phosphatidylcholine liposomes. Biochemistry 30, 31-37.
27. Yomogida, S., Nagaoka, I. & Yamashita, T. (1996) Purification of the 11- and 15-kDa antibacterial polypeptides from guinea pig neutrophils. Arch. Biochem. Biophys. 328, 219-226.
28. Amiche, M., Seon, A., Wroblewski, H. & Nicolas, P. (2000) Isolation of dermatoxin from frog skin, an antibacterial peptide encoded by a novel member of the dermaseptin gene family. Eur. J. Biochem. 267, 4583-4592.
29. 2 isoform in the gills of the red sea bream, Pagrus (Chrysophrys) major. Comp. Biochem. Physiol. 132B, 671-683.
30. Cole, A.M., Darouiche, R.O., Legarda, D., Connell, N. & Diamond, G. (2000) Characterization of a fish antimicrobial peptide: gene expression, subcellular localization, and spectrum of activity. Antimicrob. Agents Chemother. 44, 2039-2045.
31. Taylor, S.W., Craig, A.G., Fischer, W.H., Park, M. & Lehrer, R.I. (2000) Styelin D, an extensively modified antimicrobial peptide from ascidian hemocytes. J. Biol. Chem. 275, 38417-38426.
32. Vogel, H. & Jahnig, F. (1986) The structure of melittin in membranes. Biophys. J. 50, 573-582.
33. Perez-Paya, E., Dufourcq, J., Braco, L. & Abad, C. (1997) Structural characterisation of the natural membrane-bound state of melittin: a fluorescence study of a dansylated analogue. Biochim. Biophys. Acta 1329, 223-236.
34. Ladokhin, A.S. & White, S.T. (1999) Folding amphipathic α-helices on membranes: energetics of helix formation by melittin. J. Mol. Biol. 285, 1363-1369.
35. Reite, O.B. (1997) Mast cells/eosinophilic granule cells of salmonids: staining properties and responses to noxious agents. Fish Shellfish Immunol. 7, 567-584.
36. Laurent, P. (1984) Gill internal morphology. In Fish Physiology (Hoar, W.S. & Randall, D.J., eds), pp. 73-183. Academic Press, Inc, Orlando.
37. Goss, G.G., Perry, S.F., Fryer, J.N. & Lauent, P. (1998) Gill morphology and acid-base regulation in freshwater fishes. Comp. Biochem. Physiol. 119A, 107-115.