Detection of Rho GEF and GAP activity through a sensitive split luciferase assay system

Biochemical Journal

Volumn 441, Issue 3, 2012, Pages 869-879

  Anderson, Erik L ^a   Hamann, Michael J ^a  

^a BEMIDJI STATE UNIVERSITY   (United States)

Author keywords

2 (3 ) O (N methylanthraniloyl) GTP (mant GTP); Exchange assay; GTPase activating protein (GAP); Guanine nucleotide exchange factor (GEF); Protein complementation; Rho

Indexed keywords

GUANINE NUCLEOTIDE EXCHANGE FACTOR; GUANOSINE TRIPHOSPHATASE ACTIVATING PROTEIN; HYBRID PROTEIN; LUCIFERASE; P21 ACTIVATED KINASE; PROTEIN CDC42; PROTEIN P190; RAC1 PROTEIN; RECOMBINANT PROTEIN; RHOA GUANINE NUCLEOTIDE BINDING PROTEIN;

ARTICLE; BACTERIUM; CELL LYSATE; CONTROLLED STUDY; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PROCESSING; PROTEIN PURIFICATION; RADIOENZYMATIC ASSAY; SENSITIVITY AND SPECIFICITY;

CELL EXTRACTS; GENES, REPORTER; GTPASE-ACTIVATING PROTEINS; GUANINE NUCLEOTIDE EXCHANGE FACTORS; HEK293 CELLS; HUMANS; IMMUNOPRECIPITATION; JURKAT CELLS; LUCIFERASES; LUMINESCENT MEASUREMENTS; MODELS, BIOLOGICAL; MODELS, MOLECULAR; RECOMBINANT FUSION PROTEINS; SENSITIVITY AND SPECIFICITY; TRANSFECTION;

EID: 84855913119     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20111111     Document Type: Article

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