Protein kinase N (PKN) and PKN-related protein Rhophilin as targets of small GTPase Rho

Science

Volumn 271, Issue 5249, 1996, Pages 645-648

  Watanabe, Go ^a   Saito, Yuji ^a   Madaule, Pascal ^a   Ishizaki, Toshimasa ^a   Fujisawa, Kazuko ^a   Morii, Narito ^a   Mukai, Hideyuki ^b   Ono, Yoshitaka ^b   Kakizuka, Akira ^a   Narumiya, Shuh ^a  

^a KYOTO UNIVERSITY   (Japan)

^b KOBE UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATASE; GUANOSINE TRIPHOSPHATE; PROTEIN KINASE N; RHOPHILIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CELL ADHESION; CYTOKINESIS; ENZYME ACTIVATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; YEAST;

EID: 13344285358     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.271.5249.645     Document Type: Article

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36. 32P]adenosine triphosphate (ATP) was then added (0 3 μM) and the reaction was continued for 0 5 or 1.5 min at 30°C. The reaction was terminated by the addition of 50 mM EDTA and 200 μM ATP Antibody to PKN conjugated with protein A-Sepharose was then added The mixture was stirred at 4°C for 2 hours and centrifuged. The precipitates were washed and subjected to SDS-PAGE. The radioactive bands corresponding to PKN were excised and the incorporated radioactivity was determined
37. Single-letter abbreviations for the amino acid residues are as follows: A, Ala; C, Cys, D, Asp; E, Glu; F, Phe; G, Gly; H, His, I, lie, K, Lys; L, Leu, M, Met; N, Asn: P, Pro, Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp, and Y, Tyr
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43. We thank S. Hollenberg, P Bartel, S. Fields, and R Stemglanz for a yeast two-hybrid system, Y Kishimoto and K Okuyama for assistance; and M Imamura for encouragement Supported by grantsin-aid from the Ministry of Education, Science and Culture of Japan and by grants from the Human Frontier Science Program, the Senri Life Science Foundation, and the Naito Memorial Foundation. P.M. is supported by the Centre National de la Recherche Scientifique.