Use of phage display for the identification of molecular sensors specific for activated Rho

Methods in Molecular Biology

Volumn 827, Issue , 2012, Pages 283-303

  Chinestra, Patrick ^a   Lajoie Mazenc, Isabelle ^a   Faye, Jean Charles ^a   Favre, Gilles ^a  

^a UNIVERSITÉ DE TOULOUSE   (France)

Author keywords

Activated Rho; Molecular sensors; Phage display; scFv

Indexed keywords

HYBRID PROTEIN; RHO GUANINE NUCLEOTIDE BINDING PROTEIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANTIBODY SPECIFICITY; ARTICLE; ENZYME ACTIVATION; ENZYME LINKED IMMUNOSORBENT ASSAY; GENETIC PROCEDURES; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; PEPTIDE LIBRARY;

ANTIBODY SPECIFICITY; BIOSENSING TECHNIQUES; ENZYME ACTIVATION; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; PEPTIDE LIBRARY; RECOMBINANT FUSION PROTEINS; RHO GTP-BINDING PROTEINS; SINGLE-CHAIN ANTIBODIES;

EID: 84855897053     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-442-1_19     Document Type: Article

References (23)

1. Etienne-Manneville, S., and Hall, A. (2002) Rho GTPases in cell biology. Nature 420, 629-635. (Pubitemid 36764489)
2. Vega, F.M., and Ridley, A.J. (2008) Rho GTPases in cancer cell biology. FEBS Lett 582, 2093-2101.
3. Aznar, S., Fernandez-Valeron, P., Espina, C., and Lacal, J.C. (2004) Rho GTPases: potential candidates for anticancer therapy. Cancer Lett 206, 181-191. (Pubitemid 38317288)
4. Gomez del Pulgar, T., Benitah, S.A., Valeròn, P.F., Espina, C., and Lacal, J.C. (2005) Rho GTPase expression in tumourigenesis: evidence for a signifi cant link. Bioessays 27, 602-613. (Pubitemid 40896091)
5. Ren, X.D., Kiosses ,W.B., and Schwartz, M.A. (1999) Regulation of the small GTP binding protein Rho by cell adhesion and the cytoskeleton. EMBO J 18, 578-585. (Pubitemid 29057242)
6. Sander, E.E., van Delft, S., ten Klooster, J.P., Reid, T., van der Kammen, R.A., Michiels, F., and Collard, J.G. (1998) Matrix-dependent Tiam1/Rac signaling in epithelial cells promotes either cell-cell adhesion or cell migration and is regulated by phosphatidylinositol 3-kinase. J Cell Biol 143, 1385-1398. (Pubitemid 28559838)
7. Goulimari, P., Kitzing, T.M., Knieling, H., Brandt, D.T., Offermanns, S., and Grosse, R. (2005) G a 12/13 is essential for directed cell migration and localized Rho-Dia1 function. J Biol Chem 280, 42242-42251. (Pubitemid 43023195)
8. Bement, W.M., Benink, H.A., and von Dassow, G. (2005) A microtubule-dependent zone of active RhoA during cleavage plane specifi cation. J Cell Biol 170, 91-101. (Pubitemid 40994099)
9. Berger, C.D., Marz, M., Kitzing, T. M., Grosse, R., Steinbeisser, H. (2009) Detection of activated Rho in fi xed Xenopus tissue. Dev Dyn 238, 1407-1411.
10. Cascone, I., Audero, E., Giraudo, E., Napione, L., Maniero, F., Philips, M.R., Collard, J.G., Serini, G., and Bussolino, F. (2003) Tie-2-dependent activation of RhoA and Rac1 participates in endothelial cell motility triggered by angiopoietin-1. Blood 102, 2482-2490. (Pubitemid 37193586)
11. Nizak, C., Monier, S., del Nery, E., Moutel, S., Goud, B., and Perez, F. (2003) Recombinant antibodies to the small GTPase Rab6 as conformation sensors. Science 300, 984-987. (Pubitemid 36548886)
12. Horn, I.R., Wittinghofer, A., de Bruïne, A.P., Hoogenboom, H.R. (1999) Selection of phage-displayed fab antibodies on the active conformation of ras yields a high affi nity conformation-specifi c antibody preventing the binding of c-Raf kinase to Ras. FEBS Lett 463, 115-120. (Pubitemid 30001729)
13. Longenecker, K., Read, P., Lin, S.K., Somlyo, A.P., Nakamoto, R.K., Derewenda, Z.S. (2003) Structure of a constitutively activated RhoA mutant (Q63L) at 1.55 Å resolution. Acta Crystallogr D Biol Crystallogr 59, 876-880. (Pubitemid 36599366)
14. Griffi ths, A.D., Williams, S.C., Hartley, O., Tomlinson, I.M., Waterhouse, P., Crosby, W.L., Kontermann, R.E., Jones, P.T., Low, N.M., Allison, T.J., Prospero, T.D., Hoogenboom, H.R., Nissim, A., Cox, J.P.L., Harrison, J.L., Zaccolo, M., Gherardi, E., and Winter, G. (1994) Isolation of high affi nity human antibodies directly from large synthetic repertoires. EMBO J 13, 3245-3260. (Pubitemid 24226939)
15. Goffi net, M., Chinestra, P., Lajoie-Mazenc, I., Medale-Giamarchi, C., Favre, G., and Faye, J.C. (2008) Identifi cation of a GTP-bound Rho specifi c scFv molecular sensor by phage display selection. BMC Biotechnol 8, 34-47.
16. Sheffi eld, P., Garrard, S., and Derewenda, Z. (1999) Overcoming expression and purifi cation problems of RhoGDI using a family of "parallel" expression vectors. Protein Expr Purif 15, 34-39. (Pubitemid 29321466)
17. Dubel, S., Breitling, F., Kontermann, R., Schmidt, T., Skerra, A., and Little, M. (1995) Bifunctional and multimeric complexes of streptavidin fused to single chain antibodies (scFv). J Immunol Methods 178, 201-209.
18. Knappik, A., Ge, L., Honegger, A., Pack, P., Fischer, M., Wellnhofer, G., Hoess, A., Wolle, J., Pluckthun, A., and Virnekas, B. (2000) Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides. J Mol Biol 296, 57-86.
19. Silacci, M., Brack, S., Schirru, G., Marlind, J., Ettorre, A., Merlo, A., Viti, F., and Neri, D. (2005) Design, construction, and characterization of a large synthetic human antibody phage display library. Proteomics 5, 2340-2350. (Pubitemid 40835403)
20. Philibert, P., Stoessel, A., Wang, W., Sibler, A.P., Bec, N., Larroque, C., Saven, J.G., Courtete, J., Weiss, E., and Martineau, P. (2007) A focused antibody library for selecting scFvs expressed at high levels in the cytoplasm. BMC Biotechnol 7, 81-97.
21. Tanaka, T., and Rabbitts, T.H. (2003) Intrabodies based on intracellular capture frameworks that bind the RAS protein with high affi nity and impair oncogenic transformation. EMBO J 22, 1025-1035. (Pubitemid 36313587)
22. Tanaka, T., Williams, R.L., and Rabbitts, T.H. (2007) Tumour prevention by a single antibody domain targeting the interaction of signal transduction proteins with RAS. EMBO J 26, 3250-3259. (Pubitemid 47057484)
23. Jensen, K.B., Larsen, M., Pedersen, J.S., Christensen, P.A., Alvarez-Vallina, L., Goletz, S., Clark, B.F., and Kristensen, P. (2002) Functional improvement of antibody fragments using a novel phage coat protein III fusion system. Biochem Biophys Res Commun 298, 566-573. (Pubitemid 35333695)