Recombinant antibodies to the small GTPase Rab6 as conformation sensors

Science

Volumn 300, Issue 5621, 2003, Pages 984-987

  Nizak, Clément ^a   Monier, Solange ^a   Del Nery, Elaine ^a   Moutel, Sandrine ^b   Goud, Bruno ^a   Perez, Franck ^a  

^a INSTITUT CURIE   (France)

^b INSTITUT CURIE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CONFORMATIONS; FLUORESCENCE; MOLECULES; SENSORS;

CONFORMATION SENSORS;

ANTIBODIES;

ANTIBODY; GREEN FLUORESCENT PROTEIN; GUANOSINE TRIPHOSPHATASE; GUANOSINE TRIPHOSPHATE; PROTEIN RAB6; RAB PROTEIN; UNCLASSIFIED DRUG;

ANTIBODY; BIOLOGY;

ANTIBODY SPECIFICITY; ARTICLE; CELL MEMBRANE; CELL TRANSPORT; CONFORMATION; ENZYME ACTIVATION; GOLGI COMPLEX; PRIORITY JOURNAL; PROTEIN EXPRESSION; SENSOR;

ANTIBODIES; BACTERIAL PROTEINS; ENDOPLASMIC RETICULUM; FLUORESCENT ANTIBODY TECHNIQUE; GOLGI APPARATUS; GREEN FLUORESCENT PROTEINS; GUANOSINE 5'-O-(3-THIOTRIPHOSPHATE); GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATE; HELA CELLS; HUMANS; IMMUNOGLOBULIN VARIABLE REGION; LUMINESCENT PROTEINS; MUTATION; PEPTIDE LIBRARY; PROTEIN CONFORMATION; RAB GTP-BINDING PROTEINS; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; TRANSFECTION; TRANSPORT VESICLES;

EID: 0037993790     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1083911     Document Type: Article

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27. We thank G. Winter at the UK Medical Research Council for providing the Griffin. 1 library, A. Lecrux, A.-F. Ruaud, and S. Morton for their help with some of the experiments; our colleagues (see material and methods) for the gift of materials; and V. Lallemand for helpful advice. We also acknowledge A. Echard for stimulating discussions, P. Chavrier, L. Johannes, and M. van Overbeek for careful reading of the manuscript; and A. Franklin and M. Gaye for their constant assistance. This work was supported by the CNRS, the Curie Institute (PIC immunotherapy), and the Association pour la Recherche contre le Cancer (ARC5747 and ARC5881).