Identification of new interacting partners for atypical Rho GTPases: A SILAC-based approach

Methods in Molecular Biology

Volumn 827, Issue , 2012, Pages 305-317

  Montani, Laura ^a   Bausch Fluck, Damaris ^b   Domingues, Ana Filipa ^a   Wollscheid, Bernd ^b   Relvas, João Bettencourt ^a  

^a UNIVERSITY OF PORTO   (Portugal)

^b ETH ZURICH   (Switzerland)

Author keywords

Atypical Rho GTPases; Immunoprecipitation; Interactome; Mass spectrometry; Nu PAGE gradient gel; Protein overexpression; Proteomics; SILAC; Silver staining

Indexed keywords

AMINO ACID; RHO GUANINE NUCLEOTIDE BINDING PROTEIN;

ARTICLE; CELL STRAIN HEK293; CHEMISTRY; CULTURE TECHNIQUE; GENETIC TRANSFECTION; GENETICS; HUMAN; ISOTOPE LABELING; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; PLASMID; PROTEIN ANALYSIS; PROTEIN BINDING;

AMINO ACIDS; CELL CULTURE TECHNIQUES; HEK293 CELLS; HUMANS; ISOTOPE LABELING; MASS SPECTROMETRY; PLASMIDS; PROTEIN BINDING; PROTEIN INTERACTION MAPPING; RHO GTP-BINDING PROTEINS; TRANSFECTION;

EID: 84855870204     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-442-1_20     Document Type: Article

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