Metabolic engineering of antibiotic-producing actinomycetes using in vitro transposon mutagenesis

Methods in Molecular Biology

Volumn 834, Issue , 2012, Pages 153-175

  Reeves, Andrew R ^a   Weber, J Mark ^b  

^a Research Microbiologist   (United States)

^b Fermalogic Inc   (United States)

Author keywords

Actinomycete; Aeromicrobium erythreum; Erythromycin; In vitro transposon mutagenesis; Microtiter fermentation; Protoplast transformation; Reverse engineering

Indexed keywords

ANTIINFECTIVE AGENT;

ACTINOBACTERIA; ARTICLE; BIOSYNTHESIS; GENE LIBRARY; GENE ORDER; GENETIC TRANSFORMATION; GENETICS; METABOLIC ENGINEERING; METABOLISM; METHODOLOGY; MUTAGENESIS; PLASMID; PROTOPLAST; TRANSPOSON;

ACTINOBACTERIA; ANTI-BACTERIAL AGENTS; DNA TRANSPOSABLE ELEMENTS; GENE ORDER; GENOMIC LIBRARY; METABOLIC ENGINEERING; MUTAGENESIS; PLASMIDS; PROTOPLASTS; TRANSFORMATION, GENETIC;

ACTINOBACTERIA (CLASS); AEROMICROBIUM ERYTHREUM;

EID: 84855867175     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-483-4_11     Document Type: Article

References (20)

1. Queener SW, Lively DH (1986) Screening and selection for strain improvement, pp. 155-169. In: Demain AL and Solomon NA (eds) Manual of Industrial Microbiology and Biotechnology. American Society for Microbiology, Washington, DC.
2. Vinci VA, Byng, G (1999) Strain Improvement by Non-recombinant Methods, p. 103-113. In: Demain AL and Davies JE (eds), Manual of Industrial Microbiology and Biotechnology, 2nd ed. ASM Press, Washington, DC.
3. Goryshin IY, Reznikoff WS (1998) Tn5 in vitro transposition. J Biol Chem 273 , 7367-7374. (Pubitemid 28152757)
4. Kirby JR (2007) In vivo Mutagenesis using EZ-Tn5. Methods in Enzymology 421 , 17-21.
5. Rothberg JM, Leamon JH (2008) The development and impact of 454 sequencing. Nat Biotechnol 26 , 1117-1124.
6. Alper H, Miyaoku K, Stephanopoulos G (2005) Construction of lycopene-overproducing E. coli strains by combining systematic and combinatorial gene knockout targets. Nat Biotechnol 23 , 612-616. (Pubitemid 41724901)
7. Gehring AM, Wang ST, Kearns DB, Storer NY, Losick R (2004) Novel genes that infl uence development in Streptomyces coelicolor . JBacteriol 186 , 3570-3577. (Pubitemid 38661555)
8. Reeves AR, Cernota WH, Brikun IA, Wesley RK, Weber JM (2004) Engineering precursor fl ow for increased erythromycin production in Aeromicrobium erythreum . Metab Eng 6 , 300-312. (Pubitemid 39379466)
9. Tannler S, Zamboni N, Kiraly C, Aymerich S, Sauer U (2008) Screening of Bacillus subtilis transposon mutants with altered ribofl avin production. Metab Eng 10 , 216-226.
10. Trötschel C, Kandirali S, Diaz-Achirica P, Meinhardt A, Morbach S, Krämer R, Burkovski A (2003) GltS, the sodium-coupled L-glutamate uptake system of Corynebacterium I: identifi cation of the corresponding gene and impact on L-glutamate production. Appl Microbiol Biotechnol 60 , 738-742. (Pubitemid 36286683)
11. Ikeda M, Ohnishi J, Hayashi M, Mitsuhashi S (2006) A genome-based approach to create aminimally mutated Corynebacterium glutamicum strain for effi cient L-lysine production. JInd Microbiol Biotechnol 33 , 610-615. (Pubitemid 43876123)
12. Ikeda M, Mitsuhashi S, Tanaka K, Hayashi M (2009) Re-engineering of an L-Arginine and L-Citrulline Producer of Corynebacterium glutamicum. Appl Environ Microbiol 75 ,1635-1641.
13. Reeves AR, Brikun IA, Cernota WH, Leach BI, Gonzalez MC, Weber JM (2006) Effects of methylmalonyl-CoA mutase gene knockouts on erythromycin production in carbohydratebased and oil-based fermentations of Saccharopolyspora erythraea. J Ind Microbiol Biotechnol 7 , 600-609. (Pubitemid 43876122)
14. Reeves AR, Brikun IA, Cernota WH, Leach BI, Gonzalez MC, Weber JM (2007) Engineering of the methylmalonyl-CoA metabolite node of Saccharopolyspora erythraea for increased erythromycin production. Metab Eng 9 , 293-303. (Pubitemid 46809527)
15. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
16. Hopwood DA, Bibb MJ, Chater KF, Kieser T, Bruton CJ, Kieser HM, Lydiate DJ, Smith CP, Ward JM, Schrempf H (1985) Genetic Manipulation of Streptomyces , A laboratory manual. Norwich, UK: John Innes Foundation.
17. Roberts AN, Barnett L, Brenner S (1987) Transformation of Arthrobacter and studies on the transcription of the Arthrobacter ermA gene in Streptomyces lividans and E. coli . Biochem J 243 , 431-436. (Pubitemid 17072844)
18. Oh SH, Chater KF (1997) Denaturation of circular or linear DNA facilitates targeted integrative transformation of Streptomyces coelicolor A3(2): possible relevance to other organisms. J Bacteriol 129 , 122-127. (Pubitemid 26428473)
19. Reeves AR, Seshadri R, Brikun IA, Cernota WH, Gonzalez MC, Weber JM (2008) Knockout of the erythromycin biosynthetic cluster gene, eryBI , blocks isofl avone glucoside bioconversion during erythromycin fermentations in Aeromicrobium erythreum but not in Saccharopolyspora erythraea . Appl Env Microbiol 74 , 7383-7390. (Pubitemid 352773584)
20. Kanfer I, Skinner MF, Walker RB (1998). Analysis of macrolide antibiotics. J Chromato 812 , 255-286. (Pubitemid 28300439)