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Volumn 31, Issue 1, 2012, Pages 17-23

Isolation of improved free fatty acid overproducing strains of Escherichia coli via nile red based high-throughput screening

Author keywords

biofuels; Escherichia coli; fatty acid; high throughput screen; metabolic engineering; Nile red

Indexed keywords

ACID PRODUCTS; ATTRACTIVE STRATEGIES; BIOLOGICAL PRODUCTION; FREE FATTY ACID; GENETIC SELECTION; HIGH THROUGHPUT; HIGH-THROUGHPUT SCREENING; ITERATIVE FASHION; METABOLIC ENGINEERING; NILE RED; PETRODIESEL; RENEWABLE FEEDSTOCKS; SECONDARY SCREENING; STRAIN OPTIMIZATION; THEORETICAL YIELD; TRANSPORTATION FUELS;

EID: 84855863085     PISSN: 19447442     EISSN: 19447450     Source Type: Journal    
DOI: 10.1002/ep.10599     Document Type: Conference Paper
Times cited : (15)

References (47)
  • 1
    • 53049083876 scopus 로고    scopus 로고
    • Metabolic engineering for advanced biofuels production from Escherichia coli
    • Atsumi, S., &, Liao, J.C., (2008). Metabolic engineering for advanced biofuels production from Escherichia coli. Current Opinion in Biotechnology, 19, 414-419.
    • (2008) Current Opinion in Biotechnology , vol.19 , pp. 414-419
    • Atsumi, S.1    Liao, J.C.2
  • 2
    • 57049098094 scopus 로고    scopus 로고
    • Metabolic engineering of microorganisms for biofuels production: From bugs to synthetic biology to fuels
    • Lee, S.K., Chou, H., Ham, T.S., Lee, T.S., &, Keasling, J.D., (2008). Metabolic engineering of microorganisms for biofuels production: From bugs to synthetic biology to fuels. Current Opinion in Biotechnology, 19, 556-563.
    • (2008) Current Opinion in Biotechnology , vol.19 , pp. 556-563
    • Lee, S.K.1    Chou, H.2    Ham, T.S.3    Lee, T.S.4    Keasling, J.D.5
  • 4
    • 77953044867 scopus 로고    scopus 로고
    • A process for microbial hydrocarbon synthesis: Overproduction of fatty acids in Escherichia coli and catalytic conversion to alkanes
    • Lennen, R.M., Braden, D.J., West, R.M., Dumesic, J.A., &, Pfleger, B.F., (2010). A process for microbial hydrocarbon synthesis: Overproduction of fatty acids in Escherichia coli and catalytic conversion to alkanes. Biotechnology and Bioengineering, 106, 193-202.
    • (2010) Biotechnology and Bioengineering , vol.106 , pp. 193-202
    • Lennen, R.M.1    Braden, D.J.2    West, R.M.3    Dumesic, J.A.4    Pfleger, B.F.5
  • 5
    • 0014930876 scopus 로고
    • Reduction of fatty acids to alcohols by cell-free preparations of Euglena gracilis
    • Kolattukudy, P.E., (1970). Reduction of fatty acids to alcohols by cell-free preparations of Euglena gracilis. Biochemistry, 9, 1095-1102.
    • (1970) Biochemistry , vol.9 , pp. 1095-1102
    • Kolattukudy, P.E.1
  • 7
    • 33748762752 scopus 로고    scopus 로고
    • Microdiesel: Escherichia coli engineered for fuel production
    • DOI 10.1099/mic.0.29028-0
    • Kalscheuer, R., Stolting, T., &, Steinbuchel, A., (2006). Microdiesel: Escherichia coli engineered for fuel production. Microbiology, 152, 2529-2536. (Pubitemid 44405078)
    • (2006) Microbiology , vol.152 , Issue.9 , pp. 2529-2536
    • Kalscheuer, R.1    Stolting, T.2    Steinbuchel, A.3
  • 10
    • 78650570829 scopus 로고    scopus 로고
    • Application and engineering of fatty acid biosynthesis in Escherichia coli for advanced fuels and chemicals
    • Handke, P., Lynch, S.A., &, Gill, R.T., (2011). Application and engineering of fatty acid biosynthesis in Escherichia coli for advanced fuels and chemicals. Metabolic Engineering, 13, 28-37.
    • (2011) Metabolic Engineering , vol.13 , pp. 28-37
    • Handke, P.1    Lynch, S.A.2    Gill, R.T.3
  • 11
    • 0030033704 scopus 로고    scopus 로고
    • Regulation of fatty acid elongation and initiation by acyl-acyl carrier protein in Escherichia coli
    • DOI 10.1074/jbc.271.4.1833
    • Heath, R.J., &, Rock, C.O., (1996). Regulation of fatty acid elongation and initiation by acyl acyl carrier protein in Escherichia coli. Journal of Biological Chemistry, 271, 1833-1836. (Pubitemid 26051579)
    • (1996) Journal of Biological Chemistry , vol.271 , Issue.4 , pp. 1833-1836
    • Heath, R.J.1    Rock, C.O.2
  • 12
    • 0242693130 scopus 로고    scopus 로고
    • Bacterial Membrane Lipids: Where Do We Stand?
    • DOI 10.1146/annurev.micro.57.030502.090851
    • Cronan, J.E., (2003). Bacterial membrane lipids: Where do we stand? Annual Review of Microbiology, 57, 203-224. (Pubitemid 37392941)
    • (2003) Annual Review of Microbiology , vol.57 , pp. 203-224
    • Cronan, J.E.1
  • 13
    • 77954011231 scopus 로고    scopus 로고
    • Thioesterases: A new perspective based on their primary and tertiary structures
    • Cantu, D.C., Chen, Y.F., &, Reilly, P.J., (2010). Thioesterases: A new perspective based on their primary and tertiary structures. Protein Science, 19, 1281-1295.
    • (2010) Protein Science , vol.19 , pp. 1281-1295
    • Cantu, D.C.1    Chen, Y.F.2    Reilly, P.J.3
  • 14
    • 0035142840 scopus 로고    scopus 로고
    • Inhibition of Escherichia coli acetyl coenzyme A carboxylase by acyl-acyl carrier protein
    • DOI 10.1128/JB.183.4.1499-1503.2001
    • Davis, M.S., &, Cronan, J.E., (2001). Inhibition of Escherichia coli acetyl coenzyme A carboxylase by acyl-acyl carrier protein. Journal of Bacteriology, 183, 1499-1503. (Pubitemid 32107753)
    • (2001) Journal of Bacteriology , vol.183 , Issue.4 , pp. 1499-1503
    • Davis, M.S.1    Cronan Jr., J.E.2
  • 15
    • 57049105699 scopus 로고    scopus 로고
    • Overproduction of free fatty acids in E. coli: Implications for biodiesel production
    • Lu, X., Vora, H., &, Khosla, C., (2008). Overproduction of free fatty acids in E. coli: Implications for biodiesel production. Metabolic Engineering, 10, 333-339.
    • (2008) Metabolic Engineering , vol.10 , pp. 333-339
    • Lu, X.1    Vora, H.2    Khosla, C.3
  • 16
    • 0014990250 scopus 로고
    • Use of mutagens in improvement of production strains of microorganisms
    • Thoma, R.W., (1971). Use of mutagens in improvement of production strains of microorganisms. Folia Microbiologica, 16, 197.
    • (1971) Folia Microbiologica , vol.16 , pp. 197
    • Thoma, R.W.1
  • 18
    • 33847083318 scopus 로고    scopus 로고
    • Global transcription machinery engineering: A new approach for improving cellular phenotype
    • DOI 10.1016/j.ymben.2006.12.002, PII S1096717606001248
    • Alper, H., &, Stephanopoulos, G., (2007). Global transcription machinery engineering: A new approach for improving cellular phenotype. Metabolic Engineering, 9, 258-267. (Pubitemid 46809525)
    • (2007) Metabolic Engineering , vol.9 , Issue.3 , pp. 258-267
    • Alper, H.1    Stephanopoulos, G.2
  • 19
    • 68949161807 scopus 로고    scopus 로고
    • Programming cells by multiplex genome engineering and accelerated evolution
    • Wang, H.H., Isaacs, F.J., Carr, P.A., Sun, Z.Z., Xu, G., Forest, C.R., &, Church, G.M., (2009). Programming cells by multiplex genome engineering and accelerated evolution. Nature, 460, 894-U133.
    • (2009) Nature , vol.460
    • Wang, H.H.1    Isaacs, F.J.2    Carr, P.A.3    Sun, Z.Z.4    Xu, G.5    Forest, C.R.6    Church, G.M.7
  • 20
    • 33747078696 scopus 로고    scopus 로고
    • Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes
    • DOI 10.1038/nbt1226, PII NBT1226
    • Pfleger, B.F., Pitera, D.J., D Smolke, C., &, Keasling, J.D., (2006). Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes. Nature Biotechnology, 24, 1027-1032. (Pubitemid 44215398)
    • (2006) Nature Biotechnology , vol.24 , Issue.8 , pp. 1027-1032
    • Pfleger, B.F.1    Pitera, D.J.2    Smolke, C.D.3    Keasling, J.D.4
  • 21
    • 22844452835 scopus 로고    scopus 로고
    • Construction of lycopene-overproducing E. coli strains by combining systematic and combinatorial gene knockout targets
    • DOI 10.1038/nbt1083
    • Alper, H., Miyaoku, K., &, Stephanopoulos, G., (2005). Construction of lycopene-overproducing E. coli strains by combining systematic and combinatorial gene knockout targets. Nature Biotechnology, 23, 612-616. (Pubitemid 41724901)
    • (2005) Nature Biotechnology , vol.23 , Issue.5 , pp. 612-616
    • Alper, H.1    Miyaoku, K.2    Stephanopoulos, G.3
  • 22
    • 39649119874 scopus 로고    scopus 로고
    • Melanin-based high-throughput screen for L-tyrosine production in Escherichia coli
    • DOI 10.1128/AEM.02448-07
    • Santos, C.N.S., &, Stephanopoulos, G., (2008). Melanin-based high-throughput screen for L-tyrosine production in Escherichia coli. Applied and Environmental Microbiology, 74, 1190-1197. (Pubitemid 351287091)
    • (2008) Applied and Environmental Microbiology , vol.74 , Issue.4 , pp. 1190-1197
    • Santos, C.N.S.1    Stephanopoulos, G.2
  • 23
    • 33845624857 scopus 로고    scopus 로고
    • Microbial sensors for small molecules: Development of a mevalonate biosensor
    • DOI 10.1016/j.ymben.2006.08.002, PII S1096717606000796
    • Pfleger, B.F., Pitera, D.J., Newman, J.D., Martin, V.J.J., &, Keasling, J.D., (2007). Microbial sensors for small molecules: Development of a mevalonate biosensor. Metabolic Engineering, 9, 30-38. (Pubitemid 44960870)
    • (2007) Metabolic Engineering , vol.9 , Issue.1 , pp. 30-38
    • Pfleger, B.F.1    Pitera, D.J.2    Newman, J.D.3    Martin, V.J.J.4    Keasling, J.D.5
  • 24
    • 0021923858 scopus 로고
    • Nile red: A selective fluorescent stain for intracellular lipid droplets
    • DOI 10.1083/jcb.100.3.965
    • Greenspan, P., Mayer, E.P., &, Fowler, S.D., (1985). Nile red: A selective fluorescent stain for intracellular lipid droplets. Journal of Cell Biology, 100, 965-973. (Pubitemid 15135357)
    • (1985) Journal of Cell Biology , vol.100 , Issue.3 , pp. 965-973
    • Greenspan, P.1    Mayer, E.P.2    Fowler, S.D.3
  • 26
    • 62349095514 scopus 로고    scopus 로고
    • A high throughput Nile red method for quantitative measurement of neutral lipids in microalgae
    • Chen, W., Zhang, C.W., Song, L.R., Sommerfeld, M., &, Hu, Q., (2009). A high throughput Nile red method for quantitative measurement of neutral lipids in microalgae. Journal of Microbiological Methods, 77, 41-47.
    • (2009) Journal of Microbiological Methods , vol.77 , pp. 41-47
    • Chen, W.1    Zhang, C.W.2    Song, L.R.3    Sommerfeld, M.4    Hu, Q.5
  • 27
    • 33847292769 scopus 로고    scopus 로고
    • Fluorescent measurement of microalgal neutral lipids
    • DOI 10.1016/j.mimet.2006.11.008, PII S0167701206003332
    • Elsey, D., Jameson, D., Raleigh, B., &, Cooney, M.J., (2007). Fluorescent measurement of microalgal neutral lipids. Journal of Microbiological Methods, 68, 639-642. (Pubitemid 46329162)
    • (2007) Journal of Microbiological Methods , vol.68 , Issue.3 , pp. 639-642
    • Elsey, D.1    Jameson, D.2    Raleigh, B.3    Cooney, M.J.4
  • 28
    • 1142273243 scopus 로고    scopus 로고
    • Rapid estimation of lipids in oleaginous fungi and yeasts using Nile red fluorescence
    • DOI 10.1016/j.mimet.2003.10.018
    • Kimura, K., Yamaoka, M., &, Kamisaka, Y., (2004). Rapid estimation of lipids in oleaginous fungi and yeasts using Nile red fluorescence. Journal of Microbiological Methods, 56, 331-338. (Pubitemid 38210337)
    • (2004) Journal of Microbiological Methods , vol.56 , Issue.3 , pp. 331-338
    • Kimura, K.1    Yamaoka, M.2    Kamisaka, Y.3
  • 29
    • 0028149560 scopus 로고
    • Alteration of the specificity and regulation of fatty acid synthesis of Escherichia coli by expression of a plant medium-chain acyl-acyl carrier protein thioesterase
    • Voelker, T.A., &, Davies, H.M., (1994). Alteration of the specificity and regulation of fatty acid synthesis of Escherichia coli by expression of a plant medium-chain acyl-acyl carrier protein thioesterase. Journal of Bacteriology, 176, 7320-7327. (Pubitemid 24364059)
    • (1994) Journal of Bacteriology , vol.176 , Issue.23 , pp. 7320-7327
    • Voelker, T.A.1    Davies, H.M.2
  • 32
    • 0003785155 scopus 로고
    • Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
    • Miller, J.H., (1972). Experiments in Molecular Genetics, Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
    • (1972) Experiments in Molecular Genetics
    • Miller, J.H.1
  • 33
    • 57049188930 scopus 로고    scopus 로고
    • Engineering Escherichia coli for the efficient conversion of glycerol to ethanol and co-products
    • Yazdani, S.S., &, Gonzalez, R., (2008). Engineering Escherichia coli for the efficient conversion of glycerol to ethanol and co-products. Metabolic Engineering, 10, 340-351.
    • (2008) Metabolic Engineering , vol.10 , pp. 340-351
    • Yazdani, S.S.1    Gonzalez, R.2
  • 34
    • 0033003760 scopus 로고    scopus 로고
    • A simple statistical parameter for use in evaluation and validation of high throughput screening assays
    • DOI 10.1177/108705719900400206
    • Zhang, J.H., Chung, T.D.Y., &, Oldenburg, K.R., (1999). A simple statistical parameter for use in evaluation and validation of high throughput screening assays. Journal of Biomolecular Screening, 4, 67-73. (Pubitemid 29278954)
    • (1999) Journal of Biomolecular Screening , vol.4 , Issue.2 , pp. 67-73
    • Zhang, J.-H.1    Chung, T.D.Y.2    Oldenburg, K.R.3
  • 35
    • 3242669872 scopus 로고    scopus 로고
    • Improved statistical methods for hit selection in high-throughput screening
    • Brideau, C., Gunter, B., Pikounis, B., &, Liaw, A., (2003). Improved statistical methods for hit selection in high-throughput screening. Journal of Biomolecular Screening, 8, 634-647.
    • (2003) Journal of Biomolecular Screening , vol.8 , pp. 634-647
    • Brideau, C.1    Gunter, B.2    Pikounis, B.3    Liaw, A.4
  • 36
    • 2942754127 scopus 로고    scopus 로고
    • Studies on three E. coli DEAD-box helicases point to an unwinding mechanism different from that of model DNA helicases
    • DOI 10.1021/bi049852s
    • Bizebard, T., Ferlenghi, I., Iost, I., &, Dreyfus, M., (2004). Studies on three E-coli DEAD-box helicases point to an unwinding mechanism different from that of model DNA helicases. Biochemistry, 43, 7857-7866. (Pubitemid 38787694)
    • (2004) Biochemistry , vol.43 , Issue.24 , pp. 7857-7866
    • Bizebard, T.1    Ferlenghi, I.2    Iost, I.3    Dreyfus, M.4
  • 38
    • 0033572636 scopus 로고    scopus 로고
    • Polyamine transport in bacteria and yeast
    • DOI 10.1042/0264-6021:3440633
    • Igarashi, K., &, Kashiwagi, K., (1999). Polyamine transport in bacteria and yeast. Biochemical Journal, 344, 633-642. (Pubitemid 30032511)
    • (1999) Biochemical Journal , vol.344 , Issue.3 , pp. 633-642
    • Igarashi, K.1    Kashiwagi, K.2
  • 39
    • 0036708003 scopus 로고    scopus 로고
    • The beta-barrel finder (BBF) program, allowing identification of outer membrane beta-barrel proteins encoded within prokaryotic genomes
    • Zhai, Y.F., &, Saier, M.H., (2002). The beta-barrel finder (BBF) program, allowing identification of outer membrane beta-barrel proteins encoded within prokaryotic genomes. Protein Science, 11, 2196-2207.
    • (2002) Protein Science , vol.11 , pp. 2196-2207
    • Zhai, Y.F.1    Saier, M.H.2
  • 40
    • 65349091597 scopus 로고    scopus 로고
    • A periplasmic aldehyde oxidoreductase represents the first molybdopterin cytosine dinucleotide cofactor containing molybdo-flavoenzyme from Escherichia coli
    • Neumann, M., Mittelstadt, G., Iobbi-Nivol, C., Saggu, M., Lendzian, F., Hildebrandt, P., &, Leimkuhler, S., (2009). A periplasmic aldehyde oxidoreductase represents the first molybdopterin cytosine dinucleotide cofactor containing molybdo-flavoenzyme from Escherichia coli. FEBS Journal, 276, 2762-2774.
    • (2009) FEBS Journal , vol.276 , pp. 2762-2774
    • Neumann, M.1    Mittelstadt, G.2    Iobbi-Nivol, C.3    Saggu, M.4    Lendzian, F.5    Hildebrandt, P.6    Leimkuhler, S.7
  • 41
    • 84855881300 scopus 로고
    • Association of bothenoyl-coA hydratase and 3-hydroxyacyl-coA epimerase with an active-site in the n-terminal domain of the multifunctional fatty acid oxidation protein from Escherichia coli
    • Yang, S.Y., &, Elzinga, M., (1993). Association of bothenoyl-coA hydratase and 3-hydroxyacyl-coA epimerase with an active-site in the n-terminal domain of the multifunctional fatty acid oxidation protein from Escherichia coli. FASEB Journal, 7, A1277-A1277.
    • (1993) FASEB Journal , vol.7
    • Yang, S.Y.1    Elzinga, M.2
  • 42
    • 2142653130 scopus 로고    scopus 로고
    • Molecular biology of microbial hydrogenases
    • Vignais, P.M., &, Colbeau, A., (2004). Molecular biology of microbial hydrogenases. Current Issues in Molecular Biology, 6, 159-188. (Pubitemid 38541487)
    • (2004) Current Issues in Molecular Biology , vol.6 , Issue.2 , pp. 159-188
    • Vignais, P.M.1    Colbeau, A.2
  • 43
    • 33744722488 scopus 로고    scopus 로고
    • Escherichia coli competence gene homologs are essential for competitive fitness and the use of DNA as a nutrient
    • DOI 10.1128/JB.01974-05
    • Palchevskiy, V., &, Finkel, S.E., (2006). Escherichia coli competence gene homologs are essential for competitive fitness and the use of DNA as a nutrient. Journal of Bacteriology, 188, 3902-3910. (Pubitemid 43825395)
    • (2006) Journal of Bacteriology , vol.188 , Issue.11 , pp. 3902-3910
    • Palchevskiy, V.1    Finkel, S.E.2
  • 44
    • 33644841796 scopus 로고    scopus 로고
    • RNase R affects gene expression in stationary phase: Regulation of ompA
    • DOI 10.1111/j.1365-2958.2006.05092.x
    • Andrade, J.M., Cairrao, F., &, Arraiano, C.M., (2006). RNase R affects gene expression in stationary phase: Regulation of ompA. Molecular Microbiology, 60, 219-228. (Pubitemid 43363300)
    • (2006) Molecular Microbiology , vol.60 , Issue.1 , pp. 219-228
    • Andrade, J.M.1    Cairrao, F.2    Arraiano, C.M.3
  • 45
    • 0027999821 scopus 로고
    • Use of transposon TnphoA to identify genes for cell envelope proteins of Escherichia coli required for long-chain fatty acid transport: The periplasmic protein Tsp potentiates long-chain fatty acid transport
    • Azizan, A., &, Black, P.N., (1994). Use of transposon tnphoa to identify genes for cell-envelope proteins of Escherichia coli required for long-chain fatty-acid transport-the periplasmic protein tsp potentiates long-chain fatty-acid transport. Journal of Bacteriology, 176, 6653-6662. (Pubitemid 24338080)
    • (1994) Journal of Bacteriology , vol.176 , Issue.21 , pp. 6653-6662
    • Azizan, A.1    Black, P.N.2
  • 46
    • 0037240787 scopus 로고    scopus 로고
    • A new Escherichia coli metabolic competency: Growth on fatty acids by a novel anaerobic b-oxidation pathway
    • DOI 10.1046/j.1365-2958.2003.03341.x
    • Campbell, J.W., Morgan-Kiss, R.M., &, Cronan, J.E., (2003). A new Escherichia coli metabolic competency: Growth on fatty acids by a novel anaerobic beta-oxidation pathway. Molecular Microbiology, 47, 793-805. (Pubitemid 36114986)
    • (2003) Molecular Microbiology , vol.47 , Issue.3 , pp. 793-805
    • Campbell, J.W.1    Morgan-Kiss, R.M.2    Cronan Jr., J.E.3
  • 47
    • 0036282337 scopus 로고    scopus 로고
    • The enigmatic Escherichia coli fadE gene is yafH
    • DOI 10.1128/JB.184.13.3759-3764.2002
    • Campbell, J.W., &, Cronan, J.E., (2002). The enigmatic Escherichia coli fadE gene is yafH. Journal of Bacteriology, 184, 3759-3764. (Pubitemid 34625690)
    • (2002) Journal of Bacteriology , vol.184 , Issue.13 , pp. 3759-3764
    • Campbell, J.W.1    Cronan Jr., J.E.2


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