Efficient and versatile one-step affinity purification of in vivo biotinylated proteins: Expression, characterization and structure analysis of recombinant human glutamate carboxypeptidase II

Protein Expression and Purification

Volumn 82, Issue 1, 2012, Pages 106-115

  Tykvart, J ^a,b   Sacha P ^a,b   Barinka C ^c   Knedlik T ^a,b   Starkova J ^a   Lubkowski, J ^d   Konvalinka, J ^a,b  

^a INSTITUTE OF ORGANIC CHEMISTRY AND BIOCHEMISTRY   (Czech Republic)

^b CHARLES UNIVERSITY   (Czech Republic)

^c INSTITUTE OF BIOTECHNOLOGY   (Czech Republic)

^d NATIONAL CANCER INSTITUTE   (United States)

Author keywords

Affinity purification; Biotin acceptor peptide; Biotin protein ligase (BirA); Co localization; PSMA; Recombinant protein expression

Indexed keywords

BIOTIN; GLUTAMATE CARBOXYPEPTIDASE II; GLUTAMATE CARBOXYPEPTIDASE II, HUMAN; MEMBRANE ANTIGEN; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BIOTINYLATION; CELL LINE; CHEMICAL STRUCTURE; CHEMISTRY; CYTOLOGY; DROSOPHILA; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; ANIMALS; ANTIGENS, SURFACE; BIOTIN; BIOTINYLATION; CELL LINE; CRYSTALLOGRAPHY, X-RAY; DROSOPHILA; ESCHERICHIA COLI; GENE EXPRESSION; GLUTAMATE CARBOXYPEPTIDASE II; HUMANS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS;

ESCHERICHIA COLI; EUKARYOTA; PROKARYOTA;

EID: 84855464771     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.11.016     Document Type: Article

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