The guanine cap of human guanylate-binding protein 1 is responsible for dimerization and self-activation of GTP hydrolysis

FEBS Journal

Volumn 279, Issue 2, 2012, Pages 203-210

  Wehner, Mark ^a   Kunzelmann, Simone ^a,b   Herrmann, Christian ^a  

^a RUHR UNIVERSITY BOCHUM   (Germany)

^b NATIONAL INSTITUTE FOR MEDICAL RESEARCH   (United Kingdom)

Author keywords

dynamin; GTPase; guanine cap; guanylate binding protein; interferons

Indexed keywords

ARGININE; ASPARTIC ACID; DIMER; GUANINE; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANINE NUCLEOTIDE BINDING PROTEIN 1; GUANOSINE TRIPHOSPHATE; LYSINE; OLIGOMER; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; DIMERIZATION; DISSOCIATION CONSTANT; ENZYME ACTIVITY; GENE MUTATION; HYDROLYSIS; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN STRUCTURE;

AMINO ACID MOTIFS; AMINO ACID SUBSTITUTION; ARGININE; BINDING SITES; DATABASES, PROTEIN; DIMERIZATION; ENZYME ACTIVATION; GTP-BINDING PROTEINS; GUANINE; GUANOSINE TRIPHOSPHATE; HUMANS; HYDROLYSIS; KINETICS; LYSINE; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; PROTEIN BINDING; PROTEIN CONFORMATION; RECOMBINANT PROTEINS;

EID: 84855454189     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2011.08415.x     Document Type: Article

References (29)

1. Bourne HR, Sanders DA, &, McCormick F, (1990) The GTPase superfamily: a conserved switch for diverse cell functions. Nature 348, 125-132.
2. Bourne HR, Sanders DA, &, McCormick F, (1991) The GTPase superfamily: conserved structure and molecular mechanism. Nature 349, 117-127. (Pubitemid 21912023)
3. Matozaki T, Nakanishi H, &, Takai Y, (2000) Small G-protein networks: their crosstalk and signal cascades. Cell Signal 12, 515-524.
4. Koelle MR, (2006) Heterotrimeric G protein signaling: getting inside the cell. Cell 126, 25-27.
5. Weijland A, Harmark K, Cool RH, Anborgh PH, &, Parmeggiani A, (1992) Elongation factor Tu: a molecular switch in protein biosynthesis. Mol Microbiol 6, 683-688.
6. van der Bliek AM, (1999) Functional diversity in the dynamin family. Trends Cell Biol 9, 96-102. (Pubitemid 29151785)
7. Praefcke GJ, &, McMahon HT, (2004) The dynamin superfamily: universal membrane tubulation and fission molecules? Nat Rev Mol Cell Biol 5, 133-147. (Pubitemid 38160256)
8. Vetter IR, &, Wittinghofer A, (2001) The guanine nucleotide-binding switch in three dimensions. Science 294, 1299-1304. (Pubitemid 33063089)
9. Herrmann C, Martin GA, &, Wittinghofer A, (1995) Quantitative analysis of the complex between p21ras and the Ras-binding domain of the human Raf-1 protein kinase. J Biol Chem 270, 2901-2905.
10. Bos JL, Rehmann H, &, Wittinghofer A, (2007) GEFs and GAPs: critical elements in the control of small G proteins. Cell 129, 865-877. (Pubitemid 46802708)
11. Labrousse AM, Zappaterra MD, Rube DA, &, van der Bliek AM, (1999) C. elegans dynamin-related protein DRP-1 controls severing of the mitochondrial outer membrane. Mol Cell 4, 815-826.
12. Smirnova E, Griparic L, Shurland DL, &, van der Bliek AM, (2001) Dynamin-related protein Drp1 is required for mitochondrial division in mammalian cells. Mol Biol Cell 12, 2245-2256. (Pubitemid 33051954)
13. Anderson SL, Carton JM, Lou J, Xing L, &, Rubin BY, (1999) Interferon-induced guanylate binding protein-1 (GBP-1) mediates an antiviral effect against vesicular stomatitis virus and encephalomyocarditis virus. Virology 256, 8-14. (Pubitemid 29391821)
14. Guenzi E, Töpolt K, Cornali E, Lubeseder-Martellato C, Jörg A, Matzen K, Zietz C, Kremmer E, Nappi F, Schwemmle M, et al. (2001) The helical domain of GBP-1 mediates the inhibition of endothelial cell proliferation by inflammatory cytokines. EMBO J 20, 5568-5577. (Pubitemid 33010034)
15. Guenzi E, Töpolt K, Lubeseder-Martellato C, Jörg A, Naschberger E, Benelli R, Albini A, &, Stürzl M, (2003) The guanylate binding protein-1 GTPase controls the invasive and angiogenic capability of endothelial cells through inhibition of MMP-1 expression. EMBO J 22, 3772-3782. (Pubitemid 36975706)
16. Kim BH, Shenoy AR, Kumar P, Das R, Tiwari S, &, MacMicking JD, (2011) A family of IFN-gamma-inducible 65-kD GTPases protects against bacterial infection. Science 332, 717-721.
17. Prakash B, Praecke GJ, Renault L, Wittinghofer A, &, Herrmann C, (2000) Structure of human guanylate-binding protein 1 representing a unique class of GTP-binding proteins. Nature 403, 567-571. (Pubitemid 30082205)
18. Schwemmle M, &, Staeheli P, (1994) The interferon-induced 67-kDa guanylate-binding protein (hGBP1) is a GTPase that converts GTP to GMP. J Biol Chem 269, 11299-11305. (Pubitemid 24200342)
19. Praefcke GJ, Geyer M, Schwemmle M, Kalbitzer HR, &, Herrmann C, (1999) Nucleotide-binding characteristics of human guanylate-binding protein 1 (hGBP1) and identification of the third GTP-binding motif. J Mol Biol 292, 321-332. (Pubitemid 29435769)
20. Kunzelmann S, Praefcke GJ, &, Herrmann C, (2006) Transient kinetic investigation of GTP hydrolysis catalyzed by interferon-γ-induced hGBP1 (human guanylate binding protein 1). J Biol Chem 281, 28627-28635. (Pubitemid 44507005)
21. Ghosh A, Praefcke GJ, Renault L, Wittinghofer A, &, Herrmann C, (2006) How guanylate-binding proteins achieve assembly-stimulated processive cleavage of GTP to GMP. Nature 440, 101-104. (Pubitemid 43336275)
22. Praefcke GJ, Kloep S, Benscheid U, Lilie H, Prakash R, &, Herrmann C, (2004) Identification of residues in the human guanylate-binding protein 1 critical for nucleotide binding and cooperative GTP hydrolysis. J Mol Biol 344, 257-269. (Pubitemid 39422386)
23. Kunzelmann S, Praefcke GJ, &, Herrmann C, (2005) Nucleotide binding and self-stimulated GTPase activity of human guanylate-binding protein 1 (hGBP1). Methods Enzymol 404, 512-527.
24. Wehner M, &, Herrmann C, (2010) Biochemical properties of the human guanylate binding protein 5 and a tumor-specific truncated splice variant. FEBS J 277, 1597-1605.
25. Vöpel T, Kunzelmann S, &, Herrmann C, (2009) Nucleotide dependent cysteine reactivity of hGBP1 uncovers a domain movement during GTP hydrolysis. FEBS Lett 583, 1923-1927.
26. Vöpel T, Syguda A, Britzen-Laurent N, Kunzelmann S, Lüdemann MB, Dovengerds C, Stürzl M, &, Herrmann C, (2010) Mechanism of GTPase-activity-induced self-assembly of human guanylate binding protein 1. J Mol Biol 400, 63-70.
27. Abdullah N, Balakumari M, &, Sau AK, (2010) Dimerization and its role in GMP formation by human guanylate binding proteins. Biophys J 99, 2235-2244.
28. Britzen-Laurent N, Bauer M, Berton V, Fischer N, Syguda A, Reipschläger S, Naschberger E, Herrmann C, &, Stürzl M, (2010) Intracellular trafficking of guanylate-binding proteins is regulated by heterodimerization in a hierarchical manner. PLoS ONE 5, e14246.
29. Fres JM, Müller S, &, Praefcke GJ, (2010) Purification of the CaaX-modified, dynamin-related large GTPase hGBP1 by coexpression with farnesyltransferase. J Lipid Res 51, 2454-2459.