Cordysobin, a novel alkaline serine protease with HIV-1 reverse transcriptase inhibitory activity from the medicinal mushroom Cordyceps sobolifera

Journal of Bioscience and Bioengineering

Volumn 113, Issue 1, 2012, Pages 42-47

  Wang, Shou Xian ^a,b   Liu, Yu ^b   Zhang, Guo Qing ^c   Zhao, Shuang ^b   Xu, Feng ^b   Geng, Xiao Li ^b   Wang, He Xiang ^a  

^a CHINA AGRICULTURAL UNIVERSITY   (China)

^b INSTITUTE OF PLANT AND ENVIRONMENT PROTECTION   (China)

^c BEIJING UNIVERSITY OF AGRICULTURE   (China)

Author keywords

Alkaline serine protease; Cordyceps sobolifera; Fruiting bodies; HIV 1 reverse transcriptase; Mushroom; Purification

Indexed keywords

CORDYCEPS SOBOLIFERA; FRUITING BODIES; HIV-1 REVERSE TRANSCRIPTASE; MUSHROOM; SERINE PROTEASE;

AMINO ACIDS; CELLULOSE; CHROMATOGRAPHIC ANALYSIS; ELECTROPHORESIS; GEL PERMEATION CHROMATOGRAPHY; GELS; ION CHROMATOGRAPHY; ION EXCHANGE; OPTIMIZATION; PURIFICATION; SODIUM SULFATE;

ALKALINITY;

2 DIETHYLAMINOETHANOL; BENZYLSULFONYL FLUORIDE; CELLULOSE; CHYMOSTATIN; CORDYSOBIN; DODECYL SULFATE SODIUM; IRON; PLANT EXTRACT; RNA DIRECTED DNA POLYMERASE; RNA DIRECTED DNA POLYMERASE INHIBITOR; SEPHAROSE; SERINE PROTEINASE; UNCLASSIFIED DRUG;

ADSORPTION; ANTIVIRAL ACTIVITY; ARTICLE; CONTROLLED STUDY; CORDYCEPS SOBOLIFERA; DRUG ISOLATION; DRUG PURIFICATION; ENZYME ACTIVITY; GEL FILTRATION; HUMAN IMMUNODEFICIENCY VIRUS 1; IC 50; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR WEIGHT; MUSHROOM; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; TEMPERATURE;

ANTI-HIV AGENTS; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; CORDYCEPS; COUMARINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME STABILITY; FRUITING BODIES, FUNGAL; FUNGAL PROTEINS; HIV REVERSE TRANSCRIPTASE; HIV-1; HYDROGEN-ION CONCENTRATION; INHIBITORY CONCENTRATION 50; MASS SPECTROMETRY; MOLECULAR WEIGHT; OLIGOPEPTIDES; SERINE PROTEASES; TEMPERATURE;

BASIDIOMYCOTA; CORDYCEPS SOBOLIFERA; HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 84855351484     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2011.09.005     Document Type: Article

References (60)

1. Ngai P.H., Ng T.B. A hemolysin from the mushroom Pleurotus eryngii. Appl. Microbiol. Biotechnol. 2006, 72:1185-1191.
2. Wong J.H., Ng T.B., Cheung R.C., Ye X.J., Wang H.X., Lam S.K., Lin P., Chan Y.S., Fang E.F., Ngai P.H., other 16 authors Proteins with antifungal properties and other medicinal applications from plants and mushrooms. Appl. Microbiol. Biotechnol. 2010, 87:1221-1235.
3. Wang H.X., Ng T.B. A laccase from the medicinal mushroom Ganoderma lucidum. Appl. Microbiol. Biotechnol. 2006, 72:508-513.
4. Zhang G., Wang H., Zhang X., Ng T. Helvellisin, a novel alkaline protease from the wild ascomycete mushroom Helvella lacunosa. J. Biosci. Bioeng. 2010, 109:20-24.
5. Zhang R.Y., Zhang G.Q., Hu D.D., Wang H.X., Ng T.B. A novel ribonuclease with antiproliferative activity from fresh fruiting bodies of the edible mushroom Lyophyllum shimeiji. Biochem. Genet. 2010, 48:658-668.
6. Wosten H.A. Hydrophobins: multipurpose proteins. Annu. Rev. Microbiol. 2001, 55:625-646.
7. Zhao S., Zhao Y., Li S., Zhao J., Zhang G., Wang H., Ng T.B. A novel lectin with highly potent antiproliferative and HIV-1 reverse transcriptase inhibitory activities from the edible wild mushroom Russula delica. Glycoconj. J. 2010, 27:259-265.
8. Lam S.K., Ng T.B. Lectins: production and practical applications. Appl. Microbiol. Biotechnol. 2011, 89:45-55.
9. Kent D., Sheridan C., Tomkinson H.A., White S., Hiscott P., Grierson I. Edible mushroom (Agaricus bisporus) lectin modulates human retinal pigment epithelial cell behaviour in vitro. Exp. Eye Res. 2003, 76:213-219.
10. Kent D., Sheridan C.M., Tomkinson H.A., White S.J., Hiscott P., Yu L., Grierson I. Edible mushroom (Agaricus bisporus) lectin inhibits human retinal pigment epithelial cell proliferation in vitro. Wound Repair Regen. 2003, 11:285-291.
11. Lee W., Cho Y., Son G., Choi C. Characteristic and action pattern of alkaline protease production from Bacillus sp. Korean Biochem. J. 1991, 24:537-542.
12. Kembhavi A.A., Kulkarni A., Pant A. Salt-tolerant and thermostable alkaline protease from Bacillus subtilis NCIM no. 64. Appl. Biochem. Biotechnol. 1993, 38:83-92.
13. Gessesse A., Gashe B.A. Production of alkaline protease by an alkaliphilic bacteria isolated from an alkaline soda lake. Biotechnol. Lett. 1997, 19:479-481.
14. Anwar A., Saleemuddin M. Alkaline proteases: a review. Bioresour. Technol. 1998, 64:175-183.
15. Manachini P.L., Fortina M.G. Production in sea-water of thermostable alkaline proteases by a halotolerant strain of Bacillus licheniformis. Biotechnol. Lett. 1998, 20:565-568.
16. Gotoh T., Ono H., Kikuchi K., Nirasawa S., Takahashi S. Purification and characterization of aspartic protease derived from Sf9 insect cells. Biosci. Biotechnol. Biochem. 2010, 74:2154-2157.
17. Vaiyapuri S., Harrison R.A., Bicknell A.B., Gibbins J.M., Hutchinson G. Purification and functional characterisation of rhinocerase, a novel serine protease from the venom of Bitis gabonica rhinoceros. PloS One 2010, 5:e9687.
18. Khan H., Ali I., Khan A.U., Ahmed M., Shah Z., Saeed A., Naz R., Mustafa M.R., Abbasi A. Purification and biochemical characterization of alkaline serine protease from Caesalpinia bonducella. Nat. Prod. Commun. 2010, 5:931-934.
19. Saxena L., Iyer B.K., Ananthanarayan L. Purification of a bifunctional amylase/protease inhibitor from ragi (Eleusine coracana) by chromatography and its use as an affinity ligand. J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 2010, 878:1549-1554.
20. Nagarathnam R., Rengasamy A., Balasubramanian R. Purification and properties of cysteine protease from rhizomes of Curcuma longa (Linn.). J. Sci. Food Agric. 2010, 90:97-105.
21. Burton K.S., Partis M.D., Wood D.A., Thurston C.F. Accumulation of serine proteinase in senescent sporophores of the cultivated mushroom, Agaricus bisporus. Mycol. Res. 1997, 101:146-152.
22. Burton K.S., Smith J.F., Wood D.A., Thurston C.F. Extracellular proteinases from the mycelium of the cultivated mushroom Agaricus bisporus. Mycol. Res. 1997, 101:1341-1347.
23. Kim J.H., Kim Y.S. A fibrinolytic metalloprotease from the fruiting bodies of an edible mushroom, Armillariella mellea. Biosci. Biotechnol. Biochem. 1999, 63:2130-2136.
24. Lee S.Y., Kim J.S., Kim J.E., Sapkota K., Shen M.H., Kim S., Chun H.S., Yoo J.C., Choi H.S., Kim M.K., Kim S.J. Purification and characterization of fibrinolytic enzyme from cultured mycelia of Armillaria mellea. Protein Expr. Purif. 2005, 43:10-17.
25. Shin H.H., Choi H.S. Purification and partial characterization of a metalloprotease in Flammulina velutipes. J. Microbiol. 1998, 36:20-25.
26. Nonaka T., Ishikawa H., Tsumuraya Y., Hashimoto Y., Dohmae N. Characterization of a thermostable lysine-specific metalloendopeptidase from the fruiting bodies of a basidiomycete, Grifola frondosa. J. Biochem. 1995, 118:1014-1020.
27. Yoshimoto T., Sattar A.K., Hirose W., Tsuru D. Studies on prolyl endopeptidase from shakashimeji (Lyophyllum cinerascens): purification and enzymatic properties. J. Biochem. 1988, 104:622-627.
28. Wang H., Ng T.B. Pleureryn, a novel protease from fresh fruiting bodies of the edible mushroom Pleurotus eryngii. Biochem. Biophys. Res. Commun. 2001, 289:750-755.
29. Palmieri G., Bianco C., Cennamo G., Giardina P., Marino G., Monti M., Sannia G. Purification, characterization, and functional role of a novel extracellular protease from Pleurotus ostreatus. Appl. Environ. Microbiol. 2001, 67:2754-2759.
30. Cui L., Liu Q.H., Wang H.X., Ng T.B. An alkaline protease from fresh fruiting bodies of the edible mushroom Pleurotus citrinopileatus. Appl. Microbiol. Biotechnol. 2007, 75:81-85.
31. Kim J.H., Kim Y.S. Characterization of a metalloenzyme from a wild mushroom, Tricholoma saponaceum. Biosci. Biotechnol. Biochem. 2001, 65:356-362.
32. St.Leger R.J., Charnley A.K., Cooper R.M. Characterization of cuticle-degrading proteases produced by the entomopathogen Metarhizium anisopliae. Arch. Biochem. Biophys. 1987, 253:221-232.
33. Watanabe N., Hattori M., Yokoyama E., Isomura S., Ujita M., Hara A. Entomogenous fungi that produce 2,6-pyridine dicarboxylic acid (dipicolinic acid). J. Biosci. Bioeng. 2006, 102:365-368.
34. Fujita T., Inoue K., Yamamoto S., Ikumoto T., Sasaki S., Toyama R., Chiba K., Hoshino Y., Okumoto T. Fungal metabolites. Part 11. A potent immunosuppressive activity found in Isaria sinclairii metabolite. J. Antibiot. (Tokyo) 1994, 47:208-215.
35. Miyake Y., Kozutsumi Y., Nakamura S., Fujita T., Kawasaki T. Serine palmitoyltransferase is the primary target of a sphingosine-like immunosuppressant, ISP-1/myriocin. Biochem. Biophys. Res. Commun. 1995, 211:396-403.
36. Hojjati M.R., Li Z., Zhou H., Tang S., Huan C., Ooi E., Lu S., Jiang X.C. Effect of myriocin on plasma sphingolipid metabolism and atherosclerosis in apoE-deficient mice. J. Biol. Chem. 2005, 280:10284-10289.
37. Johansen T. Utilization of adenosine triphosphate in rat mast cells during and after secretion of histamine in response to compound 48/80. Acta Pharmacol. Toxicol. (Copenh) 1983, 53:245-249.
38. Kuo Y.C., Weng S.C., Chou C.J., Chang T.T., Tsai W.J. Activation and proliferation signals in primary human T lymphocytes inhibited by ergosterol peroxide isolated from Cordyceps cicadae. Br. J. Pharmacol. 2003, 140:895-906.
39. Kiho T., Nagai K., Miyamoto I., Watanabe T., Ukai S. Polysaccharides in fungi. XXV. Biological activities of two galactomannans from the insect-body portion of Chan hua (fungus: Cordyceps cicadae). Yakugaku Zasshi 1990, 110:286-288.
40. Takano F., Yahagi N., Yahagi R., Takada S., Yamaguchi M., Shoda S., Murase T., Fushiya S., Ohta T. The liquid culture filtrates of Paecilomyces tenuipes (Peck) Samson (=Isaria japonica Yasuda) and Paecilomyces cicadae (Miquel) Samson (=Isaria sinclairii (Berk.) Llond) regulate Th1 and Th2 cytokine response in murine Peyer's patch cells in vitro and ex vivo. Int. Immunopharmacol. 2005, 5:903-916.
41. Zhang X., Liu Q., Zhang G., Wang H., Ng T.B. Purification and molecular cloning of a serine protease from the mushroom Hypsizigus marmoreus. Process Biochem. 2010, 45:724-730.
42. Laemmli U.K., Favre M. Gel electrophoresis of proteins. J. Mol. Biol. 1973, 80:575-599.
43. Matsudaira P. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J. Biol. Chem. 1987, 262:10035-10038.
44. Hajji M., Kanoun S., Nasri M., Gharsallah N. Purification and characterization of an alkaline serine-protease produced by a new isolated Aspergillus clavatus ES1. Process Biochem. 2007, 42:791-797.
45. Park B.T., Na K.H., Jung E.C., Park J.W., Kim H.H. Antifungal and anticancer activities of a protein from the mushroom Cordyceps militaris. Korean J. Physiol. Pharmacol. 2009, 13:49-54.
46. Satake M., Omori T., Iwanaga S., Suzuki T. Studies on snake venoms. XIV. Hydrolyses of insulin B chain and glucagon by proteinase C from Agkistrodon halys blomhoffi venom. J. Biochem. 1963, 54:8-16.
47. Jiang Y., Sun L., Cai Q., Liu G., Asami Y., Kiyoshi O., Cao M. Biochemical characterization of chymotrypsins from the hepatopancreas of Japanese sea bass (Lateolabrax japonicus). J. Agric. Food Chem. 2010, 58:8069-8076.
48. Tremacoldi C.R., Monti R., Jo H.S.S., Carmona E.C. Purification and properties of an alkaline protease of Aspergillus clavatus. World J. Microbiol. Biotechnol. 2007, 23:285-299.
49. Reichard U., Buttner S., Eiffert H., Staib F., Ruchel R. Purification and characterisation of an extracellular serine proteinase from Aspergillus fumigatus and its detection in tissue. J. Med. Microbiol. 1990, 33:243-251.
50. Li H.P., Hu Z., Yuan J.L., Fan H.D., Chen W., Wang S.J., Zheng S.S., Zheng Z.L., Zou G.L. A novel extracellular protease with fibrinolytic activity from the culture supernatant of Cordyceps sinensis: purification and characterization. Phytother. Res. 2007, 21:1234-1241.
51. Shaginian K.A., Alekhina I.A., Denisova N.P. Serine proteinase from the higher basidiomycetes of Coprinus genus. Biokhimiia 1990, 55:1387-1395.
52. Burton K.S., Wood D.A., Thurston C.F., Barker P.J. Purification and characterization of a serine proteinase from senescent sporophores of the commercial mushroom Agaricus bisporus. J. Gen. Microbiol. 1993, 139:1379-1386.
53. Abdus S.A., Yoshimoto T., Tsuru D. Lyophyllum cinerascens aminopeptidase: purification and enzymatic properties. Arch. Biochem. Biophys. 1989, 274:241-250.
54. Sattar A.K., Yamamoto N., Yoshimoto T., Tsuru D. Purification and characterization of an extracellular prolyl endopeptidase from Agaricus bisporus. J. Biochem. 1990, 107:256-261.
55. Wang H.X., Ng T.B. Purification of a laccase from fruiting bodies of the mushroom Pleurotus eryngii. Appl. Microbiol. Biotechnol. 2006, 69:521-525.
56. Wang J., Wang H.X., Ng T.B. A peptide with HIV-1 reverse transcriptase inhibitory activity from the medicinal mushroom Russula paludosa. Peptides 2007, 28:560-565.
57. Wong J.H., Wang H.X., Ng T.B. Marmorin, a new ribosome inactivating protein with antiproliferative and HIV-1 reverse transcriptase inhibitory activities from the mushroom Hypsizigus marmoreus. Appl. Microbiol. Biotechnol. 2008, 81:669-674.
58. Tatsumi H., Ogawa Y., Murakami S., Ishida Y., Murakami K., Masaki A., Kawabe H., Arimura H., Nakano E., Motai H. A full length cDNA clone for the alkaline protease from Aspergillus oryzae: structural analysis and expression in Saccharomyces cerevisiae. Mol. Gen. Genet. 1989, 219:33-38.
59. Bonants P.J., Fitters P.F., Thijs H., den Belder E., Waalwijk C., Henfling J.W. A basic serine protease from Paecilomyces lilacinus with biological activity against Meloidogyne hapla eggs. Microbiology 1995, 141:775-784.
60. Benito M.J., Rodriguez M., Nunez F., Asensio M.A., Bermudez M.E., Cordoba J.J. Purification and characterization of an extracellular protease from Penicillium chrysogenum Pg222 active against meat proteins. Appl. Environ. Microbiol. 2002, 68:3532-3536.