Purification and properties of cysteine protease from rhizomes of Curcuma longa (Linn.)

Journal of the Science of Food and Agriculture

Volumn 90, Issue 1, 2010, Pages 97-105

  Nagarathnam, Radhakrishnan ^a,b   Rengasamy, Anandhan ^c   Balasubramanian, Rudresan ^a  

^a UNIVERSITY OF MADRAS   (India)

^b INDIAN INSTITUTE OF TECHNOLOGY MADRAS   (India)

^c CENTRAL INSTITUTE OF FISHERIES TECHNOLOGY   (India)

Author keywords

C.longa C.domestica; Fusarium sp.; MALDI TOF MS; Plant proteinases; Pythium aphanidermatum; Trichoderma viride; Turmeric

Indexed keywords

ALANINE; ANTIFUNGAL AGENT; CYSTEINE PROTEINASE; GLUTAMIC ACID; IODOACETIC ACID; PEPTIDE; PLANT EXTRACT; PROTEASOME; PROTEINASE INHIBITOR; VEGETABLE PROTEIN;

ARTICLE; CHEMISTRY; CURCUMA LONGA; DRUG EFFECT; ENZYMOLOGY; FUNGUS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS; RHIZOME; RICE; CURCUMA; DRUG EFFECTS;

ALANINE; ANTIFUNGAL AGENTS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CURCUMA; CYSTEINE PROTEASES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FUNGI; GLUTAMIC ACID; IODOACETIC ACID; MASS SPECTROMETRY; ORYZA SATIVA; PEPTIDES; PLANT EXTRACTS; PLANT PROTEINS; PROTEASE INHIBITORS; PROTEASOME ENDOPEPTIDASE COMPLEX; RHIZOME;

CURCUMA LONGA; FUSARIUM; FUSARIUM SP.; JAPONICA; ORYZA SATIVA; PYTHIUM; PYTHIUM APHANIDERMATUM; TRICHODERMA VIRIDE;

EID: 70450057690     PISSN: 00225142     EISSN: 10970010     Source Type: Journal    
DOI: 10.1002/jsfa.3789     Document Type: Article

References (40)

1. Vincent JL and Brewin NJ, Immunolocalization of a cysteine protease in vacuoles, vesicles, and symbiosomes of pea nodule cells. Plant Physiol 123:521-530 (2000).
2. Kim M, Hamilton SE, Guddat LW and Overall CM, Plant collagenase: unique collagenolytic activity of cysteine proteases from ginger. Biochem Biophys Acta 1770:1627-1635 (2007).
3. Huang DJ, Chen HJ, Hou WC, Chen TE, Hsu WY and Lin YH, Expression and function of a cysteine proteinase cDNA from sweet potato (Ipomoea batatas [L.] Lam 'Tainong 57') storage roots. Plant Sci 169:423-431 (2005).
4. Popovic T, Kidric M, Puizdar V and Brzin J, Purification and characterization of two cysteine proteinases from Phaseolus vulgaris leaves. Plant Physiol Biochem 36:637-645 (1998).
5. Rowan AD, Buttle DJ and Barrett AJ, The cysteine proteinases of the pineapple plant. Biochem J 266:869-887 (1990).
6. Lee KL, Albee KL, Bernasconi RJ and Edmunds T, Complete amino acid sequence of ananain and a comparison with stem bromelain and other plant cysteine proteases. Biochem J 327:199-202 (1997).
7. Pardo MF, López LM, Canals F, Avilés FX, Natalucci CL and Caffini NO, Purification of balansain I, an endopeptidase from unripe fruits of Bromelia balansae Mez (Bromeliaceae). J Agric Food Chem 48:3795-3800 (2000).
8. Lopez LMI, Sequeiros C, Natalucci CL, Brullo A, Maras B, Barra D, et al, Purification and characterization of macrodontain I, a cysteine peptidase from unripe fruits of Pseudananas macrodontes (Morr.) Harms (Bromeliaceae). Protein Express Purif 18:133-140 (2000).
9. Priolo N, Morcelle del Valle S, Arribére MC, López L and Caffini N, Isolation and characterization of a cysteine protease from the latex of Araujia hortorum fruits. J Protein Chem 19:39-49 (2000).
10. Kunakbaeva Z, Carrasco R and Rozas I, An approximation to the mechanism of inhibition of cysteine proteases: nucleophilic sulfur addition to Michael acceptors type compounds. J Mol Struct 626:209-216 (2003).
11. Konno K, Hirayama C, Nakamura M, Tateishi K, Tamura Y, Harrori M, et al, Papain protects papaya trees from herbivorous insects: role of cysteine proteases in latex. Plant J 37:370-378 (2004).
12. Mazal S, Belenghia B, Delledonneb ME and Levinea A, The involvement of cysteine proteases and protease inhibitor genes in the regulation of programmed cell death in plants. Plant Cell 11:431-444 (1999).
13. Alavarez-Fernandez M, Barrett AJ, Gerhartz B, Dando PM, Ni J and Abrahamson M, Inhibition of mammalian legumain by some cystatins is due to a novel second reactive site. J Biol Chem 274:19195-19203 (1999).
14. Purseglove JW, Tropical Crops: Monocotyledons. Longman, London (1968).
15. Ammon HPT, Anazoda MI, Safayhi H, Dhawan BN and Srimal RC, Curcumin: a potent inhibitor of leukotriene B4 formation in rat peritoneal polymorphonuclear neutrophils (PMNL). Planta Med 58:26-28 (1992).
16. Kapoor S and Priyadarsini KI, Protection of radiation induced protein damage by curcumin. Biophys Chem 92:119-126 (2001).
17. Daniel S, Limson JL, Dairam A, Watkins GMandDaya S, Through metal binding, curcumin protects against lead and cadmium-induced lipid peroxidation in rat brain. J Inorg Biochem 98:266-275 (2004).
18. Hatcher H, Planalp R, Cho J, Torti FM and Torti SV, Curcumin: from ancient medicine to current clinical trials. Cell Mol Life Sci 65:1631-1652 (2008).
19. Goel A, Kunnumakkara AB and Aggarwal BB, Curcuminas 'curecumin': from kitchen to clinic. Biochem Pharmacol 75: 787-809 (2008).
20. Rao CV, Regulation of COX and LOX by curcumin. Adv Exp Med Biol 595:213-226 (2007).
21. Mandal MN, Patlolla JM, Zheng L, Agbaga MP, Tran JT, Wicker L, et al, Curcumin protects retinal cells from light-and oxidant stress-induced cell death. Free Radic Biol Med 46:672-679 (2009).
22. Sasikumar B, Turmeric, in Handbook of Herbs and Spices, Vol. 1. ed. by Peter KV. Woodhead Publishing, Cambridge, UK (2001). pp. 297-308.
23. Negi PS, Jayaprakasha GK, Jaganmohan Rao L and Sakariah KK, Antimicrobial activity of turmeric oil: a by-product from curcumin manufacturer. Agric Food Chem 47:4297-4300 (1999).
24. Davis BJ, Disk electrophoresis: II. Method. Ann NY Acad Sci 121:404-427 (1964).
25. Bradford MM, A rapid and sensitive method for the quantification of microgram-quantities of proteins utilizing the principle of protein-dye binding. Anal Biochem 72:248-254 (1976).
26. McDonald CE and Chen LL, The Lowry modification of the folin reagent for determination of protease activity. Anal Biochem 10:175-177 (1965).
27. Hellmich S and Schanz F, Production of extracellular alkali and neutral proteases of Ustilago maydis. Exp Mycol 12:225-232 (1987).
28. Laemmli UK, Cleavage of proteins during the assembly of the head of bacteriophageT4. Nature 227:680-685 (1970).
29. Blum H, Beier H and Gross HJ, Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gel. Electrophoresis 8:93-99 (1987).
30. Vukic S and Jasminka GZ, Improvement of an in-gel tryptic digestion method for matrix-assisted laser desorption/ionization-time of flight mass spectrometry peptide mapping by use of volatile solubilizing agents. Proteomics 1:1364-1367 (2001).
31. Ishida Y, Fujita T and Asai K, New detection and separation method for amino acids by high-performance liquid chromatography. J Chromatogr 204:143-148 (1981).
32. Cavalli VS, Arribere MC, Cortadi A, Caffini NO and Priolo NS, Morrenain b I, a papain-like endopeptidase from the latex of Morrenia brachystephana Griseb (Asclepiadaceae). J Protein Chem 22:15-22 (2003).
33. Riffel A, Brandelli A, Bellato CM, Souza GHMF, Eberlin MN and Tavares FCA, Purification and characterization of a keratinolytic metalloprotease from Chryseobacterium sp. kr6. J Biotechnol 128:693-703 (2007).
34. Böckle B, Galunsky B and Muller R, Characterization of a keratinolytic serine proteinase from Streptomyces pactum DSM 40530. Appl Environ Microbiol 61:3705-3710 (1995).
35. Bressollier P, Letourneau F, Urdaci M and Verneuil B, Purification and characterization of a keratinolytic serine proteinase from Streptomyces albidoflavus. Appl Environ Microbiol 65:2570-2576 (1999).
36. Bernal C, Cairó J and Coello N, Purification and characterization of a novel exocellular keratinase from Kocuria rosea. Enzyme Microb Technol 38:49-54 (2006).
37. Nam GW, Lee DW, Lee HS, Lee NJ, Kim BJ, Choe EA, et al, Native feather degradation by Fervidobacterium islandicum AW-1, a newly isolating keratinase-producing thermophilic anaerobe. Arch Microbiol 178:538-547 (2002).
38. Nilegaonkar SS, Zambare VP, Kanekar PP, Dhakephalkar PK and Sarnaik SS, Production and partial characterization of dehairing protease from Bacillus cereus MCM B-326. Biores Technol 98: 1238-1245.
39. Barrett AJ, Rawling ND and Woessner JF, Handbook of Proteolytic Enzymes. Academic Press, New York, pp. 1033-1037 (1998).
40. Radhakrishnan N, InduceddefenceresponsesinCurcumadomesticaVal. (turmeric) against rhizome rot caused by Pythium aphanidermatum (Edson) Fitzp. by prior treatment with biotic and abiotic elicitors. PhD dissertation, University of Madras, India (2008).