A novel extracellular protease with fibrinolytic activity from the culture supernatant of Cordyceps sinensis: Purification and characterization

Phytotherapy Research

Volumn 21, Issue 12, 2007, Pages 1234-1241

  Li, Hua Ping ^a   Hu, Zheng ^a   Yuan, Jiang Lan ^a   Fan, Han Dong ^a   Chen, Wei ^a   Wang, Shi Jia ^a   Zheng, Shan Shan ^a   Zheng, Zhong Liang ^a   Zou, Guo Lin ^a  

^a WUHAN UNIVERSITY   (China)

Author keywords

Cordyceps sinensis; Fibrinolytic enzyme; Protease; Serine protease

Indexed keywords

BOVINE SERUM ALBUMIN; CASEIN; CORDICEPS SINENSIS PROTEINASE; CYSTEINE; FIBRIN; FIBRINOGEN; HUMAN SERUM ALBUMIN; POLYPEPTIDE; PROTEINASE; UNCLASSIFIED DRUG;

ALPHA CHAIN; AMINO ACID ANALYSIS; AMINO TERMINAL SEQUENCE; ARTICLE; CHINESE MEDICINE; CORDICEPS SINENSIS; CORDYCEPS; DRUG EFFICACY; DRUG PURIFICATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SUBSTRATE; FIBRINOLYSIS; HERBAL MEDICINE; MOLECULAR WEIGHT; NUCLEOTIDE SEQUENCE; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DEGRADATION; PROTEIN HYDROLYSIS; SEQUENCE ANALYSIS; SUPERNATANT; TEMPERATURE;

AMINO ACID SEQUENCE; CORDYCEPS; FIBRIN; FIBRINOGEN; FIBRINOLYSIS; HYDROGEN-ION CONCENTRATION; SERINE ENDOPEPTIDASES; SUBSTRATE SPECIFICITY; TEMPERATURE;

BOVINAE; CORDYCEPS SINENSIS; FUNGI;

EID: 37849004068     PISSN: 0951418X     EISSN: 10991573     Source Type: Journal    
DOI: 10.1002/ptr.2246     Document Type: Article

References (41)

1. Astrup T, Mullertz S. 1952. The fibrin plate method for estimating fibrinolytic activity. Arch Biochem Biophys 40: 346-351.
2. Beynon RJ, Kay J. 1978. The inactivation of native enzymes by a neutral proteinase from rat intestinal muscle. Biochem J 173: 291-298.
3. Bolumar T, Sanz Y, Aristoy MC, Toldra F. 2005. Protease B from Debaryomyces hansenii: purification and biochemical properties. Int J Food Microbiol 98: 167-177.
4. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
5. Chang CT, Fan MH, Kuo FC, Sung HY. 2000. Potent fibrinolytic enzyme from a mutant of Bacillus subtilis IMR-NK1. J Agric Food Chem 48: 3210-3216.
6. Chiou SH, Hung CC, Huang KF. 1992. Characterization of a protease with alpha- and beta-fibrinogenase activity from the Western diamondback rattlesnake, Crotalus atrox. Biochem Biophys Res Commun 187: 389-396.
7. Chiou WF, Chang PC, Chou CJ, Chen CF. 2000. Protein constituent contributes to the hypotensive and vasorelaxant activities of Cordyceps sinensis. Life Sci 66: 1369-1376.
8. Datta G, Dong A, Witt J, Tu AT. 1995. Biochemical characterization of basilase, a fibrinolytic enzyme from Crotalus basiliscus basiliscus. Arch Biochem Biophys 317: 365-373.
9. Guo YW, Chang TY, Lin KT, Liu HW, Shih KC, Cheng SH. 2001. Cloning and functional expression of the mucrosobin protein, a beta-fibrinogenase of Trimeresurus mucrosquamatus (Taiwan Habu). Protein Expr Purif 23: 483-490.
10. Hahn BS, Cho SY, Ahn MY, Kim YS. 2001. Purification and characterization of a plasmin-like protease from Tenodera sinensis (Chinese mantis). Insect Biochem Mol Biol 31: 573-581.
11. Hsu TH, Shiao LH, Hsieh C, Chang DM. 2002. A comparison of the chemical composition and bioactive ingredients of the Chinese medicinal mushroom DongChongXiaCao, its counterfeit and mimic, and fermented mycelium of Cordyceps sinensis. Food Chem 78: 463-469.
12. Hung CC, Huang KF, Chiou SH. 1994. Characterization of one novel venom protease with beta-fibrinogenase activity from the Taiwan habu (Trimeresurus mucrosquamatus): purification and cDNA sequence analysis. Biochem Biophys Res Commun 205: 1707-1715.
13. Jiao HM, Yang LX, Lu B, Wu YQ, Zhou YC. 2005. Shedaoenase, a novel fibrinogenase from the venom of Agkistrodon shedaoenthesis Zhao. Acta Biochim Biophys Sin (Shanghai) 37: 835-842.
14. Kim JH, Kim YS. 1999. A fibrinolytic metalloprotease from the fruiting bodies of an edible mushroom, Armillariella mellea. Biosci Biotechnol Biochem 63: 2130-2136.
15. Kim JH, Kim YS. 2001. Characterization of a metalloenzyme from a wild mushroom, Tricholoma saponaceum. Biosci Biotechnol Biochem 65: 356-362.
16. Kim SH, Choi NS, Lee WY. 1998. Fibrin zymography: a direct analysis of fibrinolytic enzymes on gels. Anal Biochem 263: 115-116.
17. Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
18. Lee SY, Kim JS, Kim JE et al. 2005. Purification and characterization of fibrinolytic enzyme from cultured mycelia of Armillaria mellea. Protein Expr Purif 43: 10-17.
19. Leu SF, Chien CH, Tseng CY, Kuo YM, Huang BM. 2005. The in vivo effect of Cordyceps sinensis mycelium on plasma corticosterone level in male mouse. Biol Pharm Bull 28: 1722-1725.
20. Li SP, Li P, Dong TT, Tsim KW. 2001a. Anti-oxidation activity of different types of natural Cordyceps sinensis and cultured Cordyceps mycelia. Phytomedicine 8: 207-212.
21. Li SP, Li P, Dong TT, Tsim KW. 2001b. Determination of nucleosides in natural Cordyceps sinensis and cultured Cordyceps mycelia by capillary electrophoresis. Electrophoresis 22: 144-150.
22. Li SP, Su ZR, Dong TT, Tsim KW. 2002. The fruiting body and its caterpillar host of Cordyceps sinensis show close resemblance in main constituents and anti-oxidation activity. Phytomedicine 9: 319-324.
23. Li SP, Zhao KJ, Ji ZN et al. 2003. A polysaccharide isolated from Cordyceps sinensis, a traditional Chinese medicine, protects PC12 cells against hydrogen peroxide-induced injury. Life Sci 73: 2503-2513.
24. Matsubara K, Hori K, Matsuura Y, Miyazawa K. 1999. A fibrinolytic enzyme from a marine green alga, Codium latum. Phytochemistry 52: 993-999.
25. Matsubara K, Hori K, Matsuura Y, Miyazawa K. 2000. Purification and characterization of a fibrinolytic enzyme and identification of fibrinogen clotting enzyme in a marine green alga, Codium divaricatum. Comp Biochem Physiol B Biochem Mol Biol 125: 137-143.
26. Mihara H, Sumi H, Yoneta T et al. 1991. A novel fibrinolytic enzyme extracted from the earthworm, Lumbricus rubellus. Jpn J Physiol 41: 461-472.
27. Mine Y, Wong AHK, Jiang B. 2005. Fibrinolytic enzymes in Asian traditional fermented foods. Food Res Int 38: 243-250.
28. Nowak J, Tsai H. 1989. Purification and properties of three endopeptidases from baker's yeast. Can J Microbiol 35: 295-303.
29. Ouyang C, Teng CM. 1976. Fibrinogenolytic enzymes of Trimeresurus mucrosquamatus venom. Biochim Biophys Acta 420: 298-308.
30. Ouyang C, Teng CM, Chen YC. 1977. Physicochemical properties of alpha- and beta-fibrinogenases of Trimeresurus mucrosquamatus venom. Biochim Biophys Acta 481: 622-630.
31. Peng Y, Huang Q, Zhang RH, Zhang YZ. 2003. Purification and characterization of a fibrinolytic enzyme produced by Bacillus amyloliquefaciens DC-4 screened from douchi, a traditional Chinese soybean food. Comp Biochem Physiol B Biochem Mol Biol 134: 45-52.
32. Peng Y, Zhang YZ. 2002a. Isolation and characterization of fibrinolytic enzyme-producing strain DC-4 from Chinese douchi and primary analysis of the enzyme property. Chin High Technol Lett 12: 30-34.
33. Peng Y, Zhang YZ. 2002b. Optimization of fermentation conditions of douchi fibrinolytic enzyme produced by Bacillus amyloliquefaciens DC-4. Chin Food Ferment Indus 28: 19-23.
34. Pinto AF, Dobrovolski R, Veiga AB, Guimaraes JA. 2004. Lonofibrase, a novel alpha-fibrinogenase from Lonomia obliqua caterpillars. Thromb Res 113: 147-154.
35. Sumi H, Hamada H, Tsushima H, Mihara H, Muraki H. 1987. A novel fibrinolytic enzyme (nattokinase) in the vegetable cheese Natto; a typical and popular soybean food in the Japanese diet. Experientia 43: 1110-1111.
36. Yamaguchi Y, Kagota S, Nakamura K, Shinozuka K, Kunitomo M. 2000. Antioxidant activity of the extracts from fruiting bodies of cultured Cordyceps sinensis. Phytother Res 14: 647-649.
37. Ye M, Hu Z, Fan Y, He L, Xia F, Zou G. 2004. Purification and characterization of an acid deoxyribonuclease from the cultured mycelia of Cordyceps sinensis. J Biochem Mol Biol 37: 466-473.
38. Zhang G, Huang Y, Bian Y, Wong JH, Ng TB, Wang H. 2006. Hypoglycemic activity of the fungi Cordyceps militaris, Cordyceps sinensis, Tricholoma mongolicum, and Omphalia lapidescens in streptozotocin-induced diabetic rats. Appl Microbiol Biotechnol 72: 1152-1156.
39. Zhang W, Yang J, Chen J, Hou Y, Han X. 2005. Immunomodulatory and antitumour effects of an exopolysaccharide fraction from cultivated Cordyceps sinensis (Chinese caterpillar fungus) on tumour-bearing mice. Biotechnol Appl Biochem 42: 9-15.
40. Zhu JS, Halpern GM, Jones K. 1998a. The scientific rediscovery of an ancient Chinese herbal medicine: Cordyceps sinensis: part I. J Altern Complement Med 4: 289-303.
41. Zhu JS, Halpern GM, Jones K. 1998b. The scientific rediscovery of a precious ancient Chinese herbal regimen: Cordyceps sinensis: part II. J Altern Complement Med 4: 429-457.