Purification of a bifunctional amylase/protease inhibitor from ragi (Eleusine coracana) by chromatography and its use as an affinity ligand

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 878, Issue 19, 2010, Pages 1549-1554

  Saxena, Lalit ^a   Iyer, Bharti K ^a   Ananthanarayan, Laxmi ^a  

^a UNIVERSITY OF MUMBAI   (India)

Author keywords

Affinity chromatography; Bifunctional amylase protease inhibitor; Gel filtration; Ion exchange chromatography; Sodium dodecyl sulphate polyacrylamide gel electrophoresis

Indexed keywords

BIFUNCTIONAL; GEL FILTRATION; ION EXCHANGE CHROMATOGRAPHY; SODIUM DODECYL SULPHATE; SODIUM DODECYL SULPHATE-POLYACRYLAMIDE GEL ELECTROPHORESIS;

AFFINITY CHROMATOGRAPHY; AMMONIUM COMPOUNDS; CHROMATOGRAPHIC ANALYSIS; ELECTROPHORESIS; GELS; ION CHROMATOGRAPHY; ION EXCHANGERS; IONS; LIGANDS; PURIFICATION;

ION EXCHANGE;

AMMONIUM SULFATE; AMYLASE INHIBITOR; LIGAND; PROTEINASE INHIBITOR;

AFFINITY CHROMATOGRAPHY; ARTICLE; CEREAL; CONTROLLED STUDY; ELEUSINE CORACANA; GEL FILTRATION; GEL PERMEATION CHROMATOGRAPHY; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR WEIGHT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION; PRIORITY JOURNAL; PROTEIN CONTENT; PROTEIN IMMOBILIZATION; PROTEIN PURIFICATION; RAGI;

AMYLASES; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ELEUSINE; ENZYME INHIBITORS; IMMOBILIZED PROTEINS; PANCREATIC ALPHA-AMYLASES; PROTEASE INHIBITORS;

ELEUSINE CORACANA; ELEUSINE CORACANA SUBSP. CORACANA;

EID: 77952323592     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jchromb.2010.04.009     Document Type: Article

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