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Volumn 46, Issue 3, 2012, Pages 817-827

Dynamics of microcystin production and quantification of potentially toxigenic Microcystis sp. using real-time PCR

Author keywords

LC MS; Microcystin; Microcystis sp.; Real time PCR

Indexed keywords

GENES; LAKES;

EID: 84855335182     PISSN: 00431354     EISSN: 18792448     Source Type: Journal    
DOI: 10.1016/j.watres.2011.11.056     Document Type: Article
Times cited : (47)

References (52)
  • 1
    • 0037092217 scopus 로고    scopus 로고
    • Rainfall, phycocyanin, and N:P ratios related to cyanobacterial blooms in a Korean large reservoir
    • Ahn C.-Y., Chung A.-S., Oh H.-M. Rainfall, phycocyanin, and N:P ratios related to cyanobacterial blooms in a Korean large reservoir. Hydrobiologia 2002, 474(1-3):117-124.
    • (2002) Hydrobiologia , vol.474 , Issue.1-3 , pp. 117-124
    • Ahn, C.-Y.1    Chung, A.-S.2    Oh, H.-M.3
  • 2
    • 79952359319 scopus 로고    scopus 로고
    • A new quantitative PCR assay for the detection of hepatotoxigenic cyanobacteria
    • Al-Tebrineh J., Gehringer M.M., Akcaalan R., Neilan B.A. A new quantitative PCR assay for the detection of hepatotoxigenic cyanobacteria. Toxicon 2011, 57(4):546-554.
    • (2011) Toxicon , vol.57 , Issue.4 , pp. 546-554
    • Al-Tebrineh, J.1    Gehringer, M.M.2    Akcaalan, R.3    Neilan, B.A.4
  • 3
    • 0002496012 scopus 로고    scopus 로고
    • Factors regulating bluegreen dominance in a reservoir directly influenced by the Asian monsoon
    • An K.-G., Jones J.R. Factors regulating bluegreen dominance in a reservoir directly influenced by the Asian monsoon. Hydrobiologia 2000, 432(1-3):37-48.
    • (2000) Hydrobiologia , vol.432 , Issue.1-3 , pp. 37-48
    • An, K.-G.1    Jones, J.R.2
  • 4
  • 5
    • 76049119924 scopus 로고    scopus 로고
    • Estimating the abundance of toxic Microcystis in the San Francisco Estuary using quantitative real-time PCR
    • Baxa D.V., Kurobe T., Ger K.A., Lehman P.W., Teh S.J. Estimating the abundance of toxic Microcystis in the San Francisco Estuary using quantitative real-time PCR. Harmful Algae 2010, 9(3):342-349.
    • (2010) Harmful Algae , vol.9 , Issue.3 , pp. 342-349
    • Baxa, D.V.1    Kurobe, T.2    Ger, K.A.3    Lehman, P.W.4    Teh, S.J.5
  • 7
    • 0000908043 scopus 로고
    • Quantitative assay for the number of chromatophores on a chromoprotein: its application to phycoerythrin and phycocyanin
    • Brody S.S., Brody M.A. Quantitative assay for the number of chromatophores on a chromoprotein: its application to phycoerythrin and phycocyanin. Biochimica et Biophysica Acta 1961, 50(2):348-352.
    • (1961) Biochimica et Biophysica Acta , vol.50 , Issue.2 , pp. 348-352
    • Brody, S.S.1    Brody, M.A.2
  • 8
    • 77956722542 scopus 로고    scopus 로고
    • The cyanotoxins
    • Academic Press, London, J.A. Callow (Ed.)
    • Carmichael W.W. The cyanotoxins. Advances in Botanical Research 1997, vol. 27. Academic Press, London, pp. 211-256. J.A. Callow (Ed.).
    • (1997) Advances in Botanical Research , vol.27 , pp. 211-256
    • Carmichael, W.W.1
  • 10
    • 0027045785 scopus 로고
    • Nitrate and organic N analyses with second derivative spectroscopy
    • Crumpton W.G., Isenhart T.M., Mitchell P.D. Nitrate and organic N analyses with second derivative spectroscopy. Limnology and Oceanography 1992, 37(4):907-913.
    • (1992) Limnology and Oceanography , vol.37 , Issue.4 , pp. 907-913
    • Crumpton, W.G.1    Isenhart, T.M.2    Mitchell, P.D.3
  • 11
    • 65649084531 scopus 로고    scopus 로고
    • The effects of temperature and nutrients on the growth and dynamics of toxic and non-toxic strains of Microcystis during cyanobacteria blooms
    • Davis T.W., Berry D.L., Boyer G.L., Gobler C.J. The effects of temperature and nutrients on the growth and dynamics of toxic and non-toxic strains of Microcystis during cyanobacteria blooms. Harmful Algae 2009, 8(5):715-725.
    • (2009) Harmful Algae , vol.8 , Issue.5 , pp. 715-725
    • Davis, T.W.1    Berry, D.L.2    Boyer, G.L.3    Gobler, C.J.4
  • 12
    • 84986837805 scopus 로고
    • Determination of total nitrogen in aqueous samples using persulfate digestion
    • D'Elia C.F., Steudler P.A., Corwin N. Determination of total nitrogen in aqueous samples using persulfate digestion. Limnology and Oceanography 1977, 22(4):760-764.
    • (1977) Limnology and Oceanography , vol.22 , Issue.4 , pp. 760-764
    • D'Elia, C.F.1    Steudler, P.A.2    Corwin, N.3
  • 13
    • 34147107554 scopus 로고    scopus 로고
    • Dynamics and short-term survival of toxic cyanobacteria species in ballast water from NOBOB vessels transiting the Great Lakes implications for HAB invasions
    • Doblin M.A., Coyne K.J., Rinta-Kanto J.M., Wilhelm S.W., Dobbs F.C. Dynamics and short-term survival of toxic cyanobacteria species in ballast water from NOBOB vessels transiting the Great Lakes implications for HAB invasions. Harmful Algae 2007, 6(4):519-530.
    • (2007) Harmful Algae , vol.6 , Issue.4 , pp. 519-530
    • Doblin, M.A.1    Coyne, K.J.2    Rinta-Kanto, J.M.3    Wilhelm, S.W.4    Dobbs, F.C.5
  • 14
    • 84870560686 scopus 로고
    • American Public Health Association, Washington, D.C., Section 4, A.D. Eaton, L.S. Clesceri, A.E. Greenberg (Eds.)
    • Standard Methods for the Examination of Water and Wastewater 1995, 75. American Public Health Association, Washington, D.C., Section 4. 19th ed. A.D. Eaton, L.S. Clesceri, A.E. Greenberg (Eds.).
    • (1995) Standard Methods for the Examination of Water and Wastewater , pp. 75
  • 15
    • 69949085893 scopus 로고    scopus 로고
    • Morphological characteristics and phylogenetic analyses of unusual morphospecies of Microcystis novacekii forming bloom in the Cheffia Dam (Algeria)
    • El Herry S., Nasri H., Bouaicha N. Morphological characteristics and phylogenetic analyses of unusual morphospecies of Microcystis novacekii forming bloom in the Cheffia Dam (Algeria). Journal of Limnology 2009, 68(2):242-250.
    • (2009) Journal of Limnology , vol.68 , Issue.2 , pp. 242-250
    • El Herry, S.1    Nasri, H.2    Bouaicha, N.3
  • 16
    • 0032190109 scopus 로고    scopus 로고
    • Optimised extraction of microcystins from field samples - a comparison of different solvents and procedures
    • Fastner J., Flieger I., Neumann U. Optimised extraction of microcystins from field samples - a comparison of different solvents and procedures. Water Research 1998, 32(10):3177-3181.
    • (1998) Water Research , vol.32 , Issue.10 , pp. 3177-3181
    • Fastner, J.1    Flieger, I.2    Neumann, U.3
  • 17
    • 50649099074 scopus 로고    scopus 로고
    • PCR-based detection of microcystin-producing cyanobacterial blooms from Central India
    • Ghosh S.K., Das P.K., Bagchi S.N. PCR-based detection of microcystin-producing cyanobacterial blooms from Central India. Indian Journal of Experimental Biology 2008, 46(1):66-70.
    • (2008) Indian Journal of Experimental Biology , vol.46 , Issue.1 , pp. 66-70
    • Ghosh, S.K.1    Das, P.K.2    Bagchi, S.N.3
  • 18
    • 4344614168 scopus 로고    scopus 로고
    • Cyanobacterial toxins in drinking water: a Canadian perspective
    • Springer-Verlag, Berlin, I. Chorus (Ed.)
    • Gupta S., Giddings M., Sheffer M. Cyanobacterial toxins in drinking water: a Canadian perspective. Cyanotoxins; Occurrence, Causes, Consequences 2001, 208-212. Springer-Verlag, Berlin. I. Chorus (Ed.).
    • (2001) Cyanotoxins; Occurrence, Causes, Consequences , pp. 208-212
    • Gupta, S.1    Giddings, M.2    Sheffer, M.3
  • 19
    • 63149119819 scopus 로고    scopus 로고
    • Quantification of toxic Microcystis and evaluation of its dominance ratio in blooms using real-time PCR
    • Ha J.H., Hidaka T., Tsuno H. Quantification of toxic Microcystis and evaluation of its dominance ratio in blooms using real-time PCR. Environmental Science and Technology 2009, 43(3):812-818.
    • (2009) Environmental Science and Technology , vol.43 , Issue.3 , pp. 812-818
    • Ha, J.H.1    Hidaka, T.2    Tsuno, H.3
  • 20
    • 0347117582 scopus 로고    scopus 로고
    • PCR-based identification of microcystin-producing genotypes of different cyanobacterial genera
    • Hisbergues M., Christiansen G., Rouhiainen L., Sivonen K., Börner T. PCR-based identification of microcystin-producing genotypes of different cyanobacterial genera. Archives of Microbiology 2003, 180(6):402-410.
    • (2003) Archives of Microbiology , vol.180 , Issue.6 , pp. 402-410
    • Hisbergues, M.1    Christiansen, G.2    Rouhiainen, L.3    Sivonen, K.4    Börner, T.5
  • 21
    • 45849135220 scopus 로고    scopus 로고
    • Spatial and temporal diversity of microcystins and microcystin-producing genotypes in Oneida Lake, NY
    • Hotto A.M., Satchwell M.F., Berry D.L., Gobler C.J., Boyer G.L. Spatial and temporal diversity of microcystins and microcystin-producing genotypes in Oneida Lake, NY. Harmful Algae 2008, 7(5):671-681.
    • (2008) Harmful Algae , vol.7 , Issue.5 , pp. 671-681
    • Hotto, A.M.1    Satchwell, M.F.2    Berry, D.L.3    Gobler, C.J.4    Boyer, G.L.5
  • 22
    • 33645936691 scopus 로고    scopus 로고
    • Microcystins associated with Microcystis dominated blooms in the Southwest wetlands, Western Australia
    • Kemp A., John J. Microcystins associated with Microcystis dominated blooms in the Southwest wetlands, Western Australia. Environmental Toxicology 2006, 21(2):125-130.
    • (2006) Environmental Toxicology , vol.21 , Issue.2 , pp. 125-130
    • Kemp, A.1    John, J.2
  • 23
    • 77956543855 scopus 로고    scopus 로고
    • Annual variation of Microcystis genotypes and their potential toxicity in water and sediment from a eutrophic reservoir
    • Kim S.-G., Joung S.-H., Ahn C.-Y., Ko S.-R., Boo S.M., Oh H.-M. Annual variation of Microcystis genotypes and their potential toxicity in water and sediment from a eutrophic reservoir. FEMS Microbiology Ecology 2010, 74(1):93-102.
    • (2010) FEMS Microbiology Ecology , vol.74 , Issue.1 , pp. 93-102
    • Kim, S.-G.1    Joung, S.-H.2    Ahn, C.-Y.3    Ko, S.-R.4    Boo, S.M.5    Oh, H.-M.6
  • 24
    • 0033771658 scopus 로고    scopus 로고
    • Role of chemical and physical variables in regulating microcystin-LR concentration in phytoplankton of eutrophic lakes
    • Kotak B.G., Lam A.K.Y., Prepas E.E., Hrudey S.E. Role of chemical and physical variables in regulating microcystin-LR concentration in phytoplankton of eutrophic lakes. Canadian Journal of Fisheries and Aquatic Sciences 2000, 57(8):1584-1593.
    • (2000) Canadian Journal of Fisheries and Aquatic Sciences , vol.57 , Issue.8 , pp. 1584-1593
    • Kotak, B.G.1    Lam, A.K.Y.2    Prepas, E.E.3    Hrudey, S.E.4
  • 25
    • 85036137632 scopus 로고    scopus 로고
    • PCR-based detection of mcy genes in blooms of Microcystis and extracellular DNA of pond water
    • Kumar A., Kumar A., Rai A.K., Tyagi M.B. PCR-based detection of mcy genes in blooms of Microcystis and extracellular DNA of pond water. African Journal of Microbiology Research 2011, 5(4):374-381.
    • (2011) African Journal of Microbiology Research , vol.5 , Issue.4 , pp. 374-381
    • Kumar, A.1    Kumar, A.2    Rai, A.K.3    Tyagi, M.B.4
  • 26
    • 0242657482 scopus 로고    scopus 로고
    • Application of real-time PCR for quantification of microcystin genotypes in a population of the toxic cyanobacterium Microcystis sp
    • Kurmayer R., Kutzenberger T. Application of real-time PCR for quantification of microcystin genotypes in a population of the toxic cyanobacterium Microcystis sp. Applied and Environmental Microbiology 2003, 69(11):6723-6730.
    • (2003) Applied and Environmental Microbiology , vol.69 , Issue.11 , pp. 6723-6730
    • Kurmayer, R.1    Kutzenberger, T.2
  • 27
    • 0033851101 scopus 로고    scopus 로고
    • Variation of microcystin content of Microcystis aeruginosa relative to medium N:P ratio and growth stage
    • Lee S.J., Jang M.-H., Kim H.-S., Yoon B.-D., Oh H.-M. Variation of microcystin content of Microcystis aeruginosa relative to medium N:P ratio and growth stage. Journal of Applied Microbiology 2000, 89(2):323-329.
    • (2000) Journal of Applied Microbiology , vol.89 , Issue.2 , pp. 323-329
    • Lee, S.J.1    Jang, M.-H.2    Kim, H.-S.3    Yoon, B.-D.4    Oh, H.-M.5
  • 28
    • 84980120212 scopus 로고
    • The measurement of total phosphorus in seawater based on the liberation of organically bound fractions by persulfate oxidation
    • Menzel D.W., Corwin N. The measurement of total phosphorus in seawater based on the liberation of organically bound fractions by persulfate oxidation. Limnology and Oceanography 1965, 10(2):280-282.
    • (1965) Limnology and Oceanography , vol.10 , Issue.2 , pp. 280-282
    • Menzel, D.W.1    Corwin, N.2
  • 29
    • 0002483795 scopus 로고
    • Relation between pigment content and photosynthetic characteristics in blue-green algae
    • Myers J., Kratz W.A. Relation between pigment content and photosynthetic characteristics in blue-green algae. Journal of General Physiology 1955, 39(1):11-22.
    • (1955) Journal of General Physiology , vol.39 , Issue.1 , pp. 11-22
    • Myers, J.1    Kratz, W.A.2
  • 30
    • 0036158898 scopus 로고    scopus 로고
    • The molecular evolution and DNA profiling of toxic cyanobacteria
    • Neilan B.A. The molecular evolution and DNA profiling of toxic cyanobacteria. Current Issues in Molecular Biology 2002, 4(1):1-11.
    • (2002) Current Issues in Molecular Biology , vol.4 , Issue.1 , pp. 1-11
    • Neilan, B.A.1
  • 32
    • 0034115536 scopus 로고    scopus 로고
    • Polyketide synthase gene coupled to the peptide synthetase module involved in the biosynthesis of the cyclic heptapeptide microcystin
    • Nishizawa T., Ueda A., Asayama M., Fujii K., Harada K.-I., Ochi K., Shirai M. Polyketide synthase gene coupled to the peptide synthetase module involved in the biosynthesis of the cyclic heptapeptide microcystin. The Journal of Biochemistry 2000, 127(5):779-789.
    • (2000) The Journal of Biochemistry , vol.127 , Issue.5 , pp. 779-789
    • Nishizawa, T.1    Ueda, A.2    Asayama, M.3    Fujii, K.4    Harada, K.-I.5    Ochi, K.6    Shirai, M.7
  • 33
    • 0343851704 scopus 로고    scopus 로고
    • Microcystin production by Microcystis aeruginosa in a phosphorus-limited chemostat
    • Oh H.-M., Lee S.J., Jang M.-H., Yoon B.-D. Microcystin production by Microcystis aeruginosa in a phosphorus-limited chemostat. Applied and Environmental Microbiology 2000, 66(1):176-179.
    • (2000) Applied and Environmental Microbiology , vol.66 , Issue.1 , pp. 176-179
    • Oh, H.-M.1    Lee, S.J.2    Jang, M.-H.3    Yoon, B.-D.4
  • 35
    • 46549087651 scopus 로고    scopus 로고
    • The molecular genetics of cyanobacterial toxicity as a basis for monitoring water quality and public health risk
    • Pearson L.A., Neilan B.A. The molecular genetics of cyanobacterial toxicity as a basis for monitoring water quality and public health risk. Current Opinion in Biotechnology 2008, 19(3):281-288.
    • (2008) Current Opinion in Biotechnology , vol.19 , Issue.3 , pp. 281-288
    • Pearson, L.A.1    Neilan, B.A.2
  • 36
    • 58249106933 scopus 로고    scopus 로고
    • Stability of toxigenic Microcystis blooms
    • Prakash S., Lawton L.A., Edwards C. Stability of toxigenic Microcystis blooms. Harmful Algae 2009, 8(3):377-384.
    • (2009) Harmful Algae , vol.8 , Issue.3 , pp. 377-384
    • Prakash, S.1    Lawton, L.A.2    Edwards, C.3
  • 37
    • 0030994088 scopus 로고    scopus 로고
    • Variation of microcystins, cyanobacterial hepatotoxins, in Anabaena spp. as a function of growth stimuli
    • Rapala J., Sivonen K., Lyra C., Niemelä S.I. Variation of microcystins, cyanobacterial hepatotoxins, in Anabaena spp. as a function of growth stimuli. Applied and Environmental Microbiology 1997, 63(6):2206-2212.
    • (1997) Applied and Environmental Microbiology , vol.63 , Issue.6 , pp. 2206-2212
    • Rapala, J.1    Sivonen, K.2    Lyra, C.3    Niemelä, S.I.4
  • 39
    • 0018409915 scopus 로고
    • Generic assignments, strain histories and properties of pure cultures of cyanobacteria
    • Rippka R., Deruelles J., Waterbury J.B., Herdman M., Stanier R.Y. Generic assignments, strain histories and properties of pure cultures of cyanobacteria. Microbiology 1979, 111(1):1-61.
    • (1979) Microbiology , vol.111 , Issue.1 , pp. 1-61
    • Rippka, R.1    Deruelles, J.2    Waterbury, J.B.3    Herdman, M.4    Stanier, R.Y.5
  • 41
    • 67349244932 scopus 로고    scopus 로고
    • Isolation and characterization of microcystin producing Microcystis from a Central Indian water bloom
    • Sangolkar L.N., Maske S.S., Muthal P.L., Kashyap S.M., Chakrabarti T. Isolation and characterization of microcystin producing Microcystis from a Central Indian water bloom. Harmful Algae 2009, 8(5):674-684.
    • (2009) Harmful Algae , vol.8 , Issue.5 , pp. 674-684
    • Sangolkar, L.N.1    Maske, S.S.2    Muthal, P.L.3    Kashyap, S.M.4    Chakrabarti, T.5
  • 42
    • 0025082840 scopus 로고
    • Effects of light, temperature, nitrate, orthophosphate, and bacteria on growth of and hepatotoxin production by Oscillatoria agardhii strains
    • Sivonen K. Effects of light, temperature, nitrate, orthophosphate, and bacteria on growth of and hepatotoxin production by Oscillatoria agardhii strains. Applied and Environmental Microbiology 1990, 56(9):2658-2666.
    • (1990) Applied and Environmental Microbiology , vol.56 , Issue.9 , pp. 2658-2666
    • Sivonen, K.1
  • 43
    • 1642349412 scopus 로고    scopus 로고
    • Screening for cyanobacterial hepatotoxins, microcystins and nodularin in environmental water samples by reversed-phase liquid chromatography-electrospray ionisation mass spectrometry
    • Spoof L., Vesterkvist P., Lindholmb T., Meriluoto J. Screening for cyanobacterial hepatotoxins, microcystins and nodularin in environmental water samples by reversed-phase liquid chromatography-electrospray ionisation mass spectrometry. Journal of Chromatography A 2003, 1020(1):105-119.
    • (2003) Journal of Chromatography A , vol.1020 , Issue.1 , pp. 105-119
    • Spoof, L.1    Vesterkvist, P.2    Lindholmb, T.3    Meriluoto, J.4
  • 44
    • 0027397937 scopus 로고
    • Adaptation of cyanobacteria to environmental stimuli: new steps towards molecular mechanisms
    • Tandeau de Marsac N., Houmard J. Adaptation of cyanobacteria to environmental stimuli: new steps towards molecular mechanisms. FEMS Microbiology Letters 1993, 104(1-2):119-189.
    • (1993) FEMS Microbiology Letters , vol.104 , Issue.1-2 , pp. 119-189
    • Tandeau de Marsac, N.1    Houmard, J.2
  • 46
    • 0028795511 scopus 로고
    • Iron stimulated toxin production in Microcystis aeruginosa
    • Utkilen H., Gjølme N. Iron stimulated toxin production in Microcystis aeruginosa. Applied and Environmental Microbiology 1995, 61(2):797-800.
    • (1995) Applied and Environmental Microbiology , vol.61 , Issue.2 , pp. 797-800
    • Utkilen, H.1    Gjølme, N.2
  • 48
    • 78649355781 scopus 로고    scopus 로고
    • Pulsed nitrogen supply induces dynamic changes in the amino acid composition and microcystin production of the harmful cyanobacterium Planktothrix agardhii
    • Van de Waal D.B., Ferreruela G., Tonk L., Donk E.V., Huisman J., Visser P.M., Matthijs H.C.P. Pulsed nitrogen supply induces dynamic changes in the amino acid composition and microcystin production of the harmful cyanobacterium Planktothrix agardhii. FEMS Microbiology Ecology 2010, 74(2):430-438.
    • (2010) FEMS Microbiology Ecology , vol.74 , Issue.2 , pp. 430-438
    • Van de Waal, D.B.1    Ferreruela, G.2    Tonk, L.3    Donk, E.V.4    Huisman, J.5    Visser, P.M.6    Matthijs, H.C.P.7
  • 49
    • 0036561208 scopus 로고    scopus 로고
    • Effect of nitrogen and phosphorus on growth of toxic and nontoxic Microcystis strains and on intracellular microcystin concentrations
    • Vézie C., Rapala J., Vaitomaa J., Seitsonen J., Sivonen K. Effect of nitrogen and phosphorus on growth of toxic and nontoxic Microcystis strains and on intracellular microcystin concentrations. Microbial Ecology 2002, 43(4):443-454.
    • (2002) Microbial Ecology , vol.43 , Issue.4 , pp. 443-454
    • Vézie, C.1    Rapala, J.2    Vaitomaa, J.3    Seitsonen, J.4    Sivonen, K.5
  • 51
    • 33845548566 scopus 로고    scopus 로고
    • Dynamics of microcystin-producing and non-microcystin-producing Microcystis populations is correlated with nitrate concentration in a Japanese lake
    • Yoshida M., Yoshida T., Takashima Y., Hosoda N., Hiroishi S. Dynamics of microcystin-producing and non-microcystin-producing Microcystis populations is correlated with nitrate concentration in a Japanese lake. FEMS Microbiology Letters 2007, 266(1):49-53.
    • (2007) FEMS Microbiology Letters , vol.266 , Issue.1 , pp. 49-53
    • Yoshida, M.1    Yoshida, T.2    Takashima, Y.3    Hosoda, N.4    Hiroishi, S.5
  • 52
    • 0026021171 scopus 로고
    • Cyclic peptide hepatotoxins from freshwater cyanobacterial (blue-green algae) water blooms collected in central China
    • Zhang Q.X., Carmichael W.W., Yu M.J., Li S.H. Cyclic peptide hepatotoxins from freshwater cyanobacterial (blue-green algae) water blooms collected in central China. Environmental Toxicology and Chemistry 1991, 10(3):313-321.
    • (1991) Environmental Toxicology and Chemistry , vol.10 , Issue.3 , pp. 313-321
    • Zhang, Q.X.1    Carmichael, W.W.2    Yu, M.J.3    Li, S.H.4


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