Application of Real-Time PCR for Quantification of Microcystin Genotypes in a Population of the Toxic Cyanobacterium Microcystis sp.

Applied and Environmental Microbiology

Volumn 69, Issue 11, 2003, Pages 6723-6730

  Kurmayer, Rainer ^a,b   Kutzenberger, Thomas ^a  

^a ERICH SCHMID INSTITUTE OF MATERIALS SCIENCE   (Austria)

^b UMWELTBUNDESAMT   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; FLUORESCENCE; LAKES;

CYANOBACTERIUM;

GENES;

CYANOGINOSIN; PHYCOCYANIN; SPACER DNA;

ANALYTIC METHOD; ARTICLE; ASSAY; CYANOBACTERIUM; GENOTYPE; GERMANY; MICROCYSTIS; NONHUMAN; POLYMERASE CHAIN REACTION; SEASONAL VARIATION; TAQ NUCLEASE ASSAY; TOXIN SYNTHESIS;

BACTERIAL PROTEINS; COLONY COUNT, MICROBIAL; CULTURE MEDIA; DNA, INTERGENIC; FLUORESCENT DYES; FRESH WATER; GENOTYPE; MICROCYSTIS; OPERON; PEPTIDE SYNTHASES; PEPTIDES, CYCLIC; PHYCOCYANIN; POLYMERASE CHAIN REACTION; SEASONS; TAQ POLYMERASE;

MICROCYSTIS; MICROCYSTIS SP.; NEGIBACTERIA;

EID: 0242657482     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.69.11.6723-6730.2003     Document Type: Article

References (32)

1. Becker, S., P. Böger, R. Oehlmann, and A. Ernst. 2000. PCR bias in ecological analysis: a case study for quantitative Taq nuclease assays in analyses of microbial communities. Appl. Environ. Microbiol. 66:4945-4953.
2. Brunberg, A.-K., and P. Blomquist. 2002. Benthic overwintering of Microcystis colonies under different environmental conditions. J. Plankton Res. 24:1247-1252.
3. Brunberg, A.-K., and P. Blomqvist. 2003. Recruitment of Microcystis (Cyanophyceae) from lake sediments: the importance of littoral inocula. J. Phycol. 39:58-63.
4. Carmichael, W. W., and P. R. Gorham. 1981. The mosaic nature of toxic blooms of cyanobacteria, p. 161-172. In W. W. Carmichael (ed.), The water environment: algal toxins and health. Plenum Press, New York, N.Y.
5. Chorus, I., and J. Bartram. 1999. Toxic cyanobacteria in water. E & FN Spon, London, United Kingdom.
6. Chorus, I., V. Niesel, J. Fastner, C. Wiedner, B. Nixdorf, and K.-E. Lindenschmidt. 2001. Environmental factors and microcystin levels in water bodies, p. 159-177. In I. Chorus (ed.), Cyanotoxins: occurrences, causes, consequences. Springer Verlag, Berlin, Germany.
7. Codd, G. A., and S. G. Bell. 1985. Eutrophication and toxic cyanobacteria in freshwaters. Water Pollut. Control 84:225-232.
8. Dittmann, E., B. A. Neilan, M. Erhard, H. von Döhren, and T. Börner. 1997. Insertional mutagenesis of a peptide synthetase gene that is responsible for hepatotoxin production in the cyanobacterium Microcystis aeruginosa PCC 7806. Mol. Microbiol. 26:779-787.
9. Fastner, J. 1999. Ph.D. thesis. Microcystins (cyanobacterial hepatotoxins) in German fresh waters: extraction, occurrence, and influence of environmental factors on microcystin production. Freie Universität Berlin, Berlin, Germany.
10. Foulds, I. V., A. Granacki, C. Xiao, U. J. Krull, A. Castle, and P. A. Horgen. 2002. Quantification of microcystin-producing cyanobacteria and E. coli in water by 5′-nuclease PCR. J. Appl. Microbiol. 93:825-834.
11. Komárek, J., and K. Anagnostidis. 1999. Cyanoprokaryota, 1. Teil Chroococcales, Gustav Fischer Verlag, Jena, Germany.
12. Kotak, B. G., A. K.-Y. Lam, E. E. Prepas, S. L. Kenefick, and S. E. Hrudey. 1995. Variability of the hepatotoxin microcystin-LR in hypereutrophic drinking water lakes. J. Phycol. 31:248-263.
13. Kotak, B. G., A. K. Y. Lam, E. E. Prepas, and S. E. Hrudey. 2000. Role of chemical and physical variables in regulating microcystin-LR concentration in phytoplankton of eutrophic lakes. Can. J. Fish. Aquat. Sci. 57:1584-1593.
14. Kurmayer, R., E. Dittmann, J. Fastner, and I. Chorus. 2002. Diversity of microcystin genes within a population of the toxic cyanobacterium Microcystis spp. in Lake Wannsee (Berlin, Germany). Microb. Ecol. 43:107-118.
15. Kurmayer, R., G. Christiansen, and I. Chorus. 2003. The abundance of microcystin-producing genotypes correlates positively with colony size in Microcystis and determines its microcystin net production in Lake Wannsee. Appl. Environ. Microbiol. 69:787-795.
16. Mann, N., and N. G. Carr. 1974. Control of macromolecular composition and cell division in the blue-green alga Anacystis nidulans. J. Gen. Microbiol. 83:399-495.
17. Marahiel, M. A., T. Stachelhaus, and H. D. Mootz. 1997. Modular peptide synthetases involved in nonribosomal peptide synthesis. Chem. Rev. 97:2651-2673.
18. Oh, H.-M., S. J. Lee, J.-H. Kim, H.-S. Kim, and B.-D. Yoon. 2001. Seasonal variation and indirect monitoring of microcystin concentrations in Daechung Reservoir, Korea. Appl. Environ. Microbiol. 67:1484-1489.
19. Ohtake, A., M. Shirai, T. Aida, N. Mori, K. I. Harada, K. Matsuura, M. Suzuki, and M. Nakano. 1989. Toxicity of Microcystis species isolated from natural blooms and purification of the toxin. Appl. Environ. Microbiol. 55:3202-3207.
20. Otsuka, S., S. Suda, S. Shibata, H. Oyaizu, S. Matsumoto, and M. M. Watanabe. 2001. A proposal for the unification of five species of the cyanobacterial genus Microcystis Kützing ex Lemmermann 1907 under the rules of the bacteriological code. Int. J. Syst. Evol. Microbiol. 51:873-879.
21. Park, H.-D., M. F. Watanabe, K.-I. Harada, H. Nagai, M. Suzuki, M. Watanabe, and H. Hayashi. 1993. Hepatotoxin (microcystin) and neurotoxin (anatoxin-a) contained in natural blooms and strains of cyanobacteria from Japanese freshwaters. Nat. Toxins 1:353-360.
22. Park, H.-D., M. F. Watanabe, K.-I. Harada, M. Suzuki, H. Hayashi, and T. Okino. 1993. Seasonal variations of Microcystis species and toxic heptapeptide microcystins in Lake Suwa. Environ. Toxicol. Water Qual. 8:425-435.
23. Reynolds, C. S. 1973. Growth and buoyancy of Microcystis aeruginosa Kütz. emend. Elenkin in a shallow eutrophic lake. Proc. R. Soc. Lond. B 184:29-50.
24. Rohrlack, T., M. Henning, and J. G. Kohl. 2001. Isolation and characterization of colony-forming Microcystis aeruginosa strains, p. 152-158. In I. Chorus (ed.), Cyanotoxins: occurrences, causes, consequences. Springer Verlag, Berlin, Germany.
25. Shirai, M., A. Ohtake, T. Sano, S. Matsumoto, T. Sakamoto, A. Sato, T. Aida, K. I. Harada, T. Shimada, M. Suzuki, and M. Nakano. 1991. Toxicity and toxins of natural blooms and isolated strains of Microcystis spp. (cyanobacteria) and improved procedure for purification of cultures. Appl. Environ. Microbiol. 57:1241-1245.
26. Sivonen, K., and G. Jones. 1999. Cyanobacterial toxins, p. 41-111. In I. Chorus and J. Bartram (ed.), Toxic cyanobacteria in water. E & FN Spon, London, United Kingdom.
27. Sokal, R. R., and F. J. Rohlf. 1995. Biometry. The principles and practice of statistics in biological research. W. H. Freeman and Company, New York, N.Y.
28. Tillett, D., E. Dittmann, M. Erhard, H. vonDöhren, T. Börner, and B. A. Neilan. 2000. Structural organization of microcystin biosynthesis in Microcystis aeruginosa PCC7806: an integrated peptide-polyketide synthetase system. Chem. Biol. 7:753-764.
29. Utermöhl, H. 1958. Zur Vervollkommnung der quantitativen Phytoplanktonmethodik. Mitt. Int. Ver. Theor. Angew. Limnol. 2:1-38.
30. Vezie, C., L. Brient, K. Sivonen, G. Bertru, J.-C. Lefeuvre, and M. Salkinoja-Salonen. 1998. Variation of microcystin content of cyanobacterial blooms and isolated strains in Lake Grand-Lieu (France). Microb. Ecol. 35:126-135.
31. Watanabe, M. 1996. Isolation, cultivation and classification of bloom-forming Microcystis in Japan, p. 13-34. In M. F. Watanabe, K. I. Harada, W. W. Carmichael, and H. Fujiki (ed.), Toxic Microcystis. CRC Press, Boca Raton, Fla.
32. Zehnder, A., and P. R. Gorham. 1960. Factors influencing the growth of Microcystis aeruginosa Kütz. emend. Elenkin. Can. J. Microbiol. 6:645-660.