Probing binding pocket of serotonin transporter by single molecular force spectroscopy on living cells

Journal of Biological Chemistry

Volumn 287, Issue 1, 2012, Pages 105-113

  Wildling, Linda ^a   Rankl, Christian ^b   Haselgrübler, Thomas ^c   Gruber, Hermann J ^a   Holy, Marion ^d   Newman, Amy Hauck ^e   Zou, Mu Fa ^e   Zhu, Rong ^a   Freissmuth, Michael ^d   Sitte, Harald H ^d   Hinterdorfer, Peter ^a,c  

^a JOHANNES KEPLER UNIVERSITY LINZ   (Austria)

^b Agilent Technologies Osterrreich GmbH   (Austria)

^c Center for Advanced Bioanalysis   (Austria)

^d MEDICAL UNIVERSITY OF VIENNA   (Austria)

^e NATIONAL INSTITUTE ON DRUG ABUSE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AFM; AFM TIP; BINDING POCKETS; BINDING REACTIONS; CROSSLINKER; ENERGY LANDSCAPE; INTERACTION ENERGIES; LIGAND BINDING; LIVING CELL; MOLECULAR DYNAMICS SIMULATIONS; MOLECULAR FORCE SPECTROSCOPY; PULLING VELOCITY; SEROTONIN TRANSPORTERS; SYNAPTIC CLEFT;

ATOMIC FORCE MICROSCOPY; BINDING SITES; DRUG INTERACTIONS; MOLECULAR DYNAMICS; RATE CONSTANTS; REACTION KINETICS;

BINDING ENERGY;

COCAINE DERIVATIVE; MFZ2 12; SEROTONIN TRANSPORTER; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; ATOMIC FORCE MICROSCOPY; BINDING SITE; CONTROLLED STUDY; DRUG BINDING; DRUG DOSAGE FORM COMPARISON; DRUG MECHANISM; DRUG STRUCTURE; DRUG TRANSPORT; HUMAN; HUMAN CELL; LIGAND BINDING; MOLECULAR DYNAMICS; MOLECULAR INTERACTION; MOLECULAR PROBE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; SPECTROSCOPY;

ANIMALS; BINDING SITES; CELL SURVIVAL; CHO CELLS; CRICETINAE; CRICETULUS; HEK293 CELLS; HUMANS; KINETICS; LIGANDS; MICROSCOPY, ATOMIC FORCE; PROTEIN BINDING; SEROTONIN PLASMA MEMBRANE TRANSPORT PROTEINS; SEROTONIN UPTAKE INHIBITORS; THERMODYNAMICS; TROPANES;

EID: 84855268688     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M111.304873     Document Type: Article

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