The cytotoxic effect of palytoxin on Caco-2 cells hinders their use for in vitro absorption studies

Food and Chemical Toxicology

Volumn 50, Issue 2, 2012, Pages 206-211

  Pelin, M ^a   Sosa, S ^a   Della Loggia, R ^a   Poli, M ^b   Tubaro, A ^a   Decorti, G ^a   Florio, C ^a  

^a UNIVERSITY OF TRIESTE   (Italy)

^b UNITED STATES ARMY MEDICAL RESEARCH INSTITUTE OF INFECTIOUS DISEASES   (United States)

Author keywords

Caco 2 cells; Cytotoxicity; Palytoxin

Indexed keywords

LACTATE DEHYDROGENASE; OUABAIN; PALYTOXIN; SUPEROXIDE;

ABSORPTION; ARTICLE; CELL DENSITY; CELL STRAIN CACO 2; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG CYTOTOXICITY; DRUG EXPOSURE; ENZYME RELEASE; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IN VITRO STUDY; MITOCHONDRION; SENSITIVITY ANALYSIS;

ABSORPTION; ACRYLAMIDES; CACO-2 CELLS; ENTEROCYTES; HUMANS; MARINE TOXINS; OUABAIN; SUPEROXIDES;

DECAPODA (CRUSTACEA); DINOPHYCEAE; OSTREOPSIS; PALYTHOA; ZOANTHIDEA;

EID: 84855234535     PISSN: 02786915     EISSN: 18736351     Source Type: Journal    
DOI: 10.1016/j.fct.2011.10.032     Document Type: Article

References (28)

1. Alcala A.C., Alcala L.C., Garth J.S., Yasumura D., Yasumoto T. Human fatality due to ingestion of the crab Demania reynaudii that contained a palytoxin-like toxin. Toxicon 1988, 26(1):105-107.
2. Aligizaki K., Katokou P., Nikplaidis G., Panou A. First episode of shellfish contamination by palytoxin-like compounds from Ostreopsis species (Aegean Sea, Greece). Toxicon 2008, 51(3):418-427.
3. Aligizaki K., Katikou P., Milandri A., Diogène J. Occurrence of palytoxin.group toxins in seafood and future strategie sto complement the present state of the art. Toxicon 2011, 57(3):390-399.
4. Bellocci M., Ronzitti G., Milandri A., Melchiorre N., Grillo C., Poletti R., Yasumoto T., Rossini G.P. A cytolytic assay for the measurement of palytoxin based on a cultured monolayer cell line. Anal. Biochem. 2008, 374(1):48-55.
5. Biganzoli E., Cavenaghi L.A., Rossi R., Brunati M.C., Nolli M.L. Use of a Caco-2 cell culture model for the characterization of intestinal absorption of antibiotics. Il Farmaco 1999, 54(9):594-599.
6. Cañete E., Diogène J. Comparative study of the use of neuroblastoma cells (Neuro-2a) and neuroblastomaxglioma hybrid cells (NG108-15) for the toxic effect quantification of marine toxins. Toxicon 2008, 52(4):541-550.
7. Ciminiello, P., Dell'Aversano, C., Dello Iacovo, E., Fattorusso, E., Forino, M., Grauso, L., Tartaglione, L., Guerrini, F., Pistocchi, R., 2010. Complex palytoxin-like profile of Ostreopsis ovata. Identification of four new ovatoxins by high-resolution liquid chromatography/mass spectrometry. Rapid Commun. Mass 1393, Spectrom. 24 (18), 2735-2744.
8. EFSA (European Food Safety Authority), 2009. Scientific opinion on marine biotoxins in shellfish - palytoxin group. EFSA J. 7(12), 1-38.
9. Ehlers A., Scholz J., These A., Hessel S., Preiss-Weigert A., Lampen A. Analysis of the passage of the marine biotoxin okadaic acid through an in vitro human gut barrier. Toxicology 2011, 279:196-202.
10. Habermann E., Chhatwal G.S. Ouabain inhibits the increase due to palytoxin of cation permeability of erythrocytes. Naunyn. Schmiedebergs. Arch. Pharmacol. 1982, 319(2):101-107.
11. Ito K., Ohkusu M., Yasumoto T. Intestinal injuries caused by experimental palytoxicosis in mice. Toxicon 1996, 34(6):643-652.
12. Ito E., Yasumoto T. Toxicological studies on palytoxin and ostreocin-D administered to mice by three different routes. Toxicon 2009, 54(3):244-251.
13. Ledreux A., Krys S., Bernard C. Suitability of the Neuro-2a cell line for the detection of palytoxin and analogues (neurotoxic phycotoxins). Toxicon 2009, 53(2):300-308.
14. Levine L., Fujiki H., Gjika H.B., Van Vunakis H. A radioimmunoassay for palytoxin. Toxicon 1988, 26(12):1115-1121.
15. Moore R.E., Dietrich R.F., Hatton B., Higa T., Scheuer P.J. Nature of the λ 263 chromopho4re in the palytoxins. J. Org. Chem. 1975, 40:540-542.
16. Munday R. Palytoxin toxicology: animal studies. Toxicon 2011, 57(3):470-477.
17. Noguchi T., Hwang D.F., Arakawa O., Daigo K., Sato S., Ozaki H., Kawai N. Palytoxin as the causative agent in the parrotfish poisoning. Progress in Venom and Toxin Research 1987, 325-335. National University of Singapore, Singapore. P. Gopalakrishnakone, C.K. Tan (Eds.).
18. Onuma Y., Satake M., Ukena T., Roux J., Chanteau S., Rasolofonirina N., Ratsimaloto M., Naoki H., Yasumoto T. Identification of putative palytoxin as the cause of clupeotoxism. Toxicon 1999, 37(1):55-65.
19. Pelin M., Zanette C., De Bortoli M., Sosa S., Della Loggia R., Tubaro A., Florio C. Effects of the marine toxin palytoxin on human skin keratinocytes: role of ionic imbalance. Toxicology 2011, 282:30-38.
20. Sambuy Y., De Angelis I., Ranaldi G., Scarino M.L., Stammati A., Zucco F. The Caco-2 cell linea s a model of the intestinal barrier: influence of cell and culture-related factors on Caco-2 cell functional characteristics. Cell. Biol. Toxicol. 2005, 21:1-26.
21. Schilling W.P., Snyder D., Sinkins W.G., Estacion M. Palytoxin-induced cell death cascade in bovine aortic endothelial cells. Am. J. Physiol. Cell. Physiol. 2006, 291(4):C657-667.
22. Sosa S., Del Favero G., De Bortoli M., Vita F., Soranzo M.R., Beltramo D., Ardizzone M., Tubaro A. Palytoxin toxicity after acute oral administration in mice. Toxicol. Lett. 2009, 191(2-3):253-259.
23. Tan C.T.T., Lee E.J.D. A fatal case of crab toxin (Lophozozymus pictor) poisoning. Asia Pac. J. Pharmacol. 1988, 3:7-9.
24. Tubaro A., Durando P., Del Favero G., Ansaldi F., Icardi G., Deeds J.R., Sosa S. Case definitions for human poisonings postulated to palytoxins exposure. Toxicon 2011, 57(3):478-495.
25. Tubaro A., Hungerford J. Toxicology of marine toxins. Veterinary Toxicology. Basic and Clinical Principles 2007, 725-752. Academic Press Elsevier, New York. R.C. Gupta (Ed.).
26. Vale-Gonzalez C., Pazos M.J., Alfonso A., Vieytes M.R., Botana L.M. Study of the neuronal effects of ouabain and palytoxin and their binding to Na, K-ATPases using an optical biosensor. Toxicon 2007, 50(4):541-552.
27. Valverde I., Lago J., Reboreda A., Vieites J.M., Cabado A.G. Characteristics of palytoxin-induced cytotoxicity in neuroblastoma cells. Toxicol. In Vitro 2008, 22(6):1432-1439.
28. Valverde I., Lago J., Vieites J.M., Cabado A.G. In vitro approaches to evaluate palytoxin-induced toxicity and cell death in intestinal cells. J. Appl. Toxicol. 2008, 28(3):294-302.