Molecular authentication of the medicinal herb Ruta graveolens (Rutaceae) and an adulterant using nuclear and chloroplast DNA markers

Genetics and Molecular Research

Volumn 10, Issue 4, 2011, Pages 2806-2816

  Al Qurainy, F ^a   Khan, S ^a   Tarroum, M ^a   Al Hemaid, F M ^a   Ali, M A ^a  

^a KING SAUD UNIVERSITY   (Saudi Arabia)

Author keywords

Chloroplast spacer sequences; Euphorbia dracunculoides; Herbal drugs; NrDNA; Ruta graveolens; SCAR marker

Indexed keywords

CELL NUCLEUS DNA; CHLOROPLAST DNA; GENOMIC DNA; INTERNAL TRANSCRIBED SPACER; RNA POLYMERASE BETA SUBUNIT; RIBOSOME DNA;

ARTICLE; DNA BARCODING; DNA MARKER; EUPHORBIA; EUPHORBIA DRACUNCULOIDES; NONHUMAN; NUCLEAR RIBOSOMAL DNA GENE; PLANT GENE; PLANT LEAF; RPOB GENE; RPOC1 GENE; RUTA GRAVEOLENS; SEQUENCE ALIGNMENT; DNA SEQUENCE; GENETIC MARKER; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; POLYMERASE CHAIN REACTION; RUTACEAE; SPECIES DIFFERENCE;

DNA, CHLOROPLAST; DNA, RIBOSOMAL; EUPHORBIA; GENETIC MARKERS; POLYMERASE CHAIN REACTION; RUTA; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SPECIES SPECIFICITY;

EID: 84855195488     PISSN: None     EISSN: 16765680     Source Type: Journal    
DOI: 10.4238/2011.November.10.3     Document Type: Article

References (21)

1. Al-Qurainy F, Khan S, Ali MA, Al-Hemaid FM, et al. (2011). Authentication of Ruta graveolens and its adulterant using internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA. Pak. J. Bot. 43: 1613-1620.
2. Alvarez I and Wendel JF (2003). Ribosomal ITS sequences and plant phylogenetic inference. Mol. Phylogenet. Evol. 29: 417-434.
3. Baldwin BG, Sanderson MJ, Porter JM, Wojciechowski MF, et al. (1995). The ITS region of nuclear ribosomal DNA: a valuable source of evidence on angiosperm phylogeny. Ann. Missouri Bot. Gard. 82: 247-277.
4. Choo BK, Moon BC, Yunui JI, Kim BB, et al. (2009). Development of SCAR markers for the discrimination of three species of medicinal plants, Angelica decursiva (Peucedanum decursivum) Peucedanum praeruptorum and Anthricus sylvestris, based on the internal transcribed spacer (ITS) sequence and random amplified polymorphic DNA (RAPD). Biol. Pharm. Bull. 32: 24-30.
5. Fugel R, Carle R and Schieber A (2005). Quality and authenticity control of fruit purées, fruit preparations and jams-a review. Trends Food Sci. Tech. 16: 433-441.
6. Gulbitti-Onarici S, Sancak C, Sumer S and Ozcan S (2009). Phylogenetic relationships of some wild wheat species based on the internal transcribed spacer sequences of nrDNA. Curr. Sci. 96: 794-800.
7. Jayasinghe R, Hai NL, Coram TE, Kong S, et al. (2009). Effectiveness of an innovative prototype subtracted diversity array (SDA) for fingerprinting plant species of medicinal importance. Planta Med. 75: 1180-1185.
8. Kethidi DR, Roden DB, Ladd TR, Krell PJ, et al. (2003). Development of SCAR markers for the DNA-based detection of the Asian long-horned beetle Anoplophora glabripennis (Motschulsky). Arch. Insect Biochem. Physiol. 52: 193-194.
9. Khan S, Qureshi MI, Kamaluddin, Tanweer A, et al. (2007). Protocol for isolation of genomic DNA from dry and fresh roots of medicinal plants suitable for RAPD and restriction digestion. Afr. J. Biotech. 6: 175-178.
10. Khan S, Mirza KJ, Tyagi MR and Abdin MZ (2011). Development of RAPD Markers for authentication of Ruta graveolens (L.) and its adulterant. MAPSB 5: 58-61.
11. Kiran U, Khan S, Mirza KJ, Ram M, et al. (2010). SCAR markers: a potential tool for authentication of herbal drugs. Fitoterapia 81: 969-976.
12. Kurtz C, Leitenberger M, Carle R and Schieber A (2010). Evaluation of fruit authenticity and determination of the fruit content of fruit products using FTNIR spectroscopy of cell wall components. Food Chem. 119: 806-812.
13. Liu Z, Liu K, Chen L, Lei S, et al. (2006). Conservation ecology of endangered species Paphiopedilum armeniacum (Orchidaceae). Acta Ecol. Sin. 26: 2791-2800.
14. Nock CJ, Waters DL, Edwards MA, Bowen SG, et al. (2011). Chloroplast genome sequences from total DNA for plant identification. Plant Biotechnol. J. 9: 328-333.
15. Rahman SZ, Latif A and Singhal KC (2003). A case report of ADR due to misidentification of an indigenous drug. Indian J. Pharmacol. 35: 128-136.
16. Song J, Yao H, Li Y, Li X, et al. (2009). Authentication of the family polygonaceae in Chinese pharmacopoeia by DNA barcoding technique. J. Ethnopharmacol. 124: 434-439.
17. Sun YW, Yu JL, Hung YS, Changa JC, et al. (2011). Development of ITS sequence based SCAR markers for discrimination of Paphiopedilum armeniacum, Paphiopedilum micranthum, Paphiopedilum delenatii and their hybrids. Sci. Hortic. 127: 405-410.
18. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, et al. (1997). The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25: 4876-4882.
19. White TJ, Bruns T, Lee S and Taylor J (1990). Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics. In: PCR Protocols: A Guide to Methods and Applications (Innis M, Gelfand D, Sninsky J and White T, eds.). Academic Press, San Diego, 315-322.
20. Zhang YB, Shaw PC, Sze CW, Wang ZT, et al. (2007). Molecular authentication of Chinese herbal materials. J. Food Drug Anal. 15: 1-9.
21. Zuo Y, Chen Z, Kondo K, Funamoto T, et al. (2011). DNA barcoding of Panax species. Planta Med. 77: 182-187.