Protocol for isolation of genomic DNA from dry and fresh roots of medicinal plants suitable for RAPD and restriction digestion

African Journal of Biotechnology

Volumn 6, Issue 3, 2007, Pages 175-178

  Khan, Salim ^a   Qureshi, M Irfan ^a   Kamaluddin, ^a   Alam, Tanweer ^a   Abdin, M Z ^a  

^a HAMDARD UNIVERSITY   (India)

Author keywords

DNA isolation; Medicinal plants; PCR amplification; Restriction digestion; Roots; Secondary metabolite

Indexed keywords

GENOMIC DNA; PLANT DNA; POLYSACCHARIDE;

ARTICLE; CONTROLLED STUDY; DNA EXTRACTION; DNA ISOLATION; DNA PURIFICATION; GENE AMPLIFICATION; MEDICINAL PLANT; NONHUMAN; PLANT ROOT; RESTRICTION MAPPING; SEPARATION TECHNIQUE;

EID: 33846941229     PISSN: None     EISSN: 16845315     Source Type: Journal    
DOI: None     Document Type: Article

References (6)

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2. Keim P, Olson TC, Shoemaker RC (1988). A rapid protocol for isolating soybean DNA. Soybean Genet Newsl. 15: 150-152.
3. Doyle JJ, Doyle JL (1990). Isolation of plant DNA from fresh tissue. Focus. 12: 13-15.
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5. Kumar A, Pushpangadan P, Mehrotra S (2003). Extraction of high-molecular weight DNA from dry root tissue of Berberis lycium suitable for RAPD. Plant. Mol. Biol. Rep 21: 309a-309d.
6. Williams JGK, Kubelik AR, Livek KT, Rafalski JA, and Tingy SV (1990). DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Res 18: 6531