Purification of CFTR for mass spectrometry analysis: Identification of palmitoylation and other post-translational modifications

Protein Engineering, Design and Selection

Volumn 25, Issue 1, 2012, Pages 7-14

  McClure, Michelle ^a,b   DeLucas, Lawrence J ^c   Wilson, Landon ^d   Ray, Marjorie ^c   Rowe, Steven M ^a,e   Wu, Xiaoyun ^e   Dai, Qun ^e   Hong, Jeong S ^a,f   Sorscher, Eric J ^a,e   Kappes, John C ^e   Barnes, Stephen ^d  

^a UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

^b UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

^c UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

^d University of Alabama at Birmingham   (United States)

^e University of Alabama at Birmingham   (United States)

^f UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

Author keywords

CFTR; mass spectrometry; palmitoylation; post translational modification; protein purification

Indexed keywords

CFTR; CYSTIC FIBROSIS; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATORS; ION CHANNEL; ION MONITORING; MASS SPECTROMETRY ANALYSIS; MOLECULAR PROCESS; OVER-EXPRESSION; PALMITOYLATION; POST-TRANSLATIONAL MODIFICATION; POST-TRANSLATIONAL MODIFICATIONS; PROTEIN MATURATION; PROTEIN PURIFICATION; PURIFIED PROTEIN; SPECIFIC SITES; TRANS-MEMBRANE PROTEINS; UBIQUITINATION;

ACYLATION; ALKYLATION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PHOSPHORYLATION; PURIFICATION;

PROTEINS;

ION CHANNEL; TRANSMEMBRANE CONDUCTANCE REGULATOR;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; HUMAN; HUMAN CELL; ION MONITORING; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; NONHUMAN; PALMITOYLATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN METHYLATION; PROTEIN PHOSPHORYLATION; PROTEIN PROCESSING; PROTEIN PURIFICATION; UBIQUITINATION;

AMINO ACID SEQUENCE; BINDING SITES; BLOTTING, WESTERN; CHROMATOGRAPHY, LIQUID; CYSTIC FIBROSIS; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; HEK293 CELLS; HUMANS; LIPOYLATION; MASS SPECTROMETRY; METHYLATION; MOLECULAR SEQUENCE DATA; MUTATION; PHOSPHORYLATION; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEOLYSIS; RECOMBINANT PROTEINS; SERINE; UBIQUITINATION;

EID: 84555194937     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzr054     Document Type: Article

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