SNAP display: In vitro protein evolution in microdroplets

Methods in Molecular Biology

Volumn 805, Issue , 2012, Pages 101-111

  Kaltenbach, Miriam ^a   Hollfelder, Florian ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Directed evolution; DNA display; Emulsion; Genotype phenotype linkage; In vitro compartmentalisation; Panning; Protein engineering; SNAP tag

Indexed keywords

DNA; DRUG DERIVATIVE; GUANINE; METHYLATED DNA PROTEIN CYSTEINE METHYLTRANSFERASE;

ARTICLE; CHEMISTRY; DIRECTED MOLECULAR EVOLUTION; ENZYME SPECIFICITY; GENETICS; METABOLISM; METHODOLOGY; MICROFLUIDIC ANALYSIS; PEPTIDE LIBRARY;

DIRECTED MOLECULAR EVOLUTION; DNA; GUANINE; MICROFLUIDIC ANALYTICAL TECHNIQUES; O(6)-METHYLGUANINE-DNA METHYLTRANSFERASE; PEPTIDE LIBRARY; SUBSTRATE SPECIFICITY;

EID: 84555189955     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-379-0_7     Document Type: Article

References (23)

1. Kaltenbach, M., Schaerli, S., and Hollfelder, F. (2009) Microdroplets -A Tool for Protein Engineering. BIOforum 13, 19-21.
2. Miller, O. J., Bernath, K., Agresti, J. J., Amitai, G., Kelly, B. T., Mastrobattista, E., Taly, V., Magdassi, S., Tawfi k, D. S., and Griffi ths, A. D. (2006) Directed evolution by in vitro compartmentalization. Nature Methods 3, 561-70. (Pubitemid 43941775)
3. Schaerli, Y., and Hollfelder, F. (2009) The potential of microfl uidic water-in-oil droplets in experimental biology. Molecular Biosystems 5, 1392-404.
4. Tawfi k, D. S., and Griffi ths, A. D. (1998) Manmade cell-like compartments for molecular evolution. Nature Biotechnology 16, 652-56. (Pubitemid 28334323)
5. Gronemeyer, T., Godin, G., and Johnsson, K. (2005) Adding value to fusion proteins through covalent labelling. Current Opinion in Biotechnology 16, 453-58. (Pubitemid 41111532)
6. Keppler, A., Gendreizig, S., Gronemeyer, T., Pick, H., Vogel, H., and Johnsson, K. (2003) A general method for the covalent labeling of fusion proteins with small molecules in vivo. Nat Biotechnol 21, 86-9. (Pubitemid 36055833)
7. Keppler, A., Kindermann, M., Gendreizig, S., Pick, H., Vogel, H., and Johnsson, K. (2004) Labeling of fusion proteins of O-6-alkylguanine-DNA alkyltransferase with small molecules in vivo and in vitro. Methods 32, 437-44. (Pubitemid 38299335)
8. Williams, N. H., Takasaki, B., Wall, M., and Chin, J. (1999) Structure and nuclease activity of simple dinuclear metal complexes: Quantitative dissection of the role of metal ions. Accounts of Chemical Research 32, 485-93.
9. Schroeder, G. K., Lad, C., Wyman, P., Williams, N. H., and Wolfenden, R. (2006) The time required for water attack at the phosphorus atom of simple phosphodiesters and of DNA. Proceedings of the National Academy of Sciences of the United States of America 103, 4052-55.
10. Kaltenbach, M., Stein, V., Hollfelder, F. (2011) SNAP Dendrimers: Multivalent Protein Display on Dendrimer-Like DNA for Directed Evolution. Chembiochem 12, 2208-16.
11. Stein, V., Sielaff, I., Johnsson, K., and Hollfelder, F. (2007) A covalent chemical genotypephenotype linkage for in vitro protein evolution. Chembiochem 8, 2191-94.
12. Bertschinger, J., Grabulovski, D., and Neri, D. (2007) Selection of single domain binding proteins by covalent DNA display. Protein Engineering Design & Selection 20, 57-68. (Pubitemid 46410870)
13. Bertschinger, J., and Neri, D. (2004) Covalent DNA display as a novel tool for directed evolution of proteins in vitro. Protein Engineering Design & Selection 17, 699-707. (Pubitemid 40601890)
14. Doi, N., and Yanagawa, H. (1999) STABLE: protein-DNA fusion system for screening of combinatorial protein libraries in vitro. FEBS Lett 457, 227-30. (Pubitemid 29401933)
15. Yonezawa, M., Doi, N., Higashinakagawa, T., and Yanagawa, H. (2004) DNA display of biologically active proteins for in vitro protein selection. J Biochem 135, 285-8. (Pubitemid 38506753)
16. Yonezawa, M., Doi, N., Kawahashi, Y., Higashinakagawa, T., and Yanagawa, H. (2003) DNA display for in vitro selection of diverse peptide libraries. Nucleic Acids Res 31, e118.
17. Stein, V., Sielaff, I., Johnsson, K., and Hollfelder, F. (2007) A covalent chemical genotypephenotype linkage for in vitro protein evolution. Chembiochem 8, 2191-94.
18. Juillerat, A., Gronemeyer, T., Keppler, A., Gendreizig, S., Pick, H., Vogel, H., and Johnsson, K. (2003) Directed evolution of O-6-alkylguanine-DNA alkyltransferase for effi -cient labeling of fusion proteins with small molecules in vivo. Chemistry & Biology 10, 313-17. (Pubitemid 36516649)
19. Gronemeyer, T., Chidley, C., Juillerat, A., Heinis, C., and Johnsson, K. (2006) Directed evolution of O6-alkylguanine-DNA alkyltransferase for applications in protein labeling. Protein Eng Des Sel 19, 309-16. (Pubitemid 43999641)
20. Juillerat, A., Heinis, C., Sielaff, I., Barnikow, J., Jaccard, H., Kunz, B., Terskikh, A., and Johnsson, K. (2005) Engineering substrate specifi city of O-6-alkylguanine-DNA alkyltransferase for specifi c protein labeling in living cells. Chembiochem 6, 1263-69. (Pubitemid 40978188)
21. Stein, V., and Hollfelder, F. (2009) An Efficient method to assemble linear DNA templates for in vitro screening and selection systems. Nucleic Acids Res 37, e122.
22. Stein, V., Kaltenbach, M., and Hollfelder, F. (2011) in "Functional Genomics, Second Edition" (Kaufmann, M., and C., K., Eds.), Humana Press.
23. Stein, V. (2008) A protein display system based on human O 6 -alkylguanine alkyltransferase and in vitro compartmentalisation (Doctoral Thesis), Cambridge.