Relationship of DNA damage signaling to DNA replication following treatment with DNA topoisomerase inhibitors camptothecin/topotecan, mitoxantrone, or etoposide

Cytometry Part A

Volumn 81 A, Issue 1, 2012, Pages 45-51

  Zhao, Hong ^a   Rybak, Paulina ^b   Dobrucki, Jurek ^b   Traganos, Frank ^a   Darzynkiewicz, Zbigniew ^a  

^a NEW YORK MEDICAL COLLEGE   (United States)

^b JAGIELLONIAN UNIVERSITY   (Poland)

Author keywords

Cell cycle; Click chemistry; DNA damage response; EdU incorporation; H2AX phosphorylation; S phase

Indexed keywords

CAMPTOTHECIN; DNA TOPOISOMERASE INHIBITOR; ETOPOSIDE; H2AFX PROTEIN, HUMAN; HISTONE; MITOXANTRONE; TOPOTECAN;

ARTICLE; DNA DAMAGE; DNA REPLICATION; HUMAN; METABOLISM; PHOSPHORYLATION; SIGNAL TRANSDUCTION; TUMOR CELL LINE;

CAMPTOTHECIN; CELL LINE, TUMOR; DNA DAMAGE; DNA REPLICATION; ETOPOSIDE; HISTONES; HUMANS; MITOXANTRONE; PHOSPHORYLATION; SIGNAL TRANSDUCTION; TOPOISOMERASE INHIBITORS; TOPOTECAN;

EID: 83855162134     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.21172     Document Type: Article

References (46)

1. Salerno S, Da Settimo F, Taliani S, Simorini F La Motta C, Fornaciari G, Marini A. Recent advances in the development of topoisomerase I and II inhibitors as anticancer drugs. Curr Med Chem 2010; 17: 4270-4290.
2. Pommier Y, Leo E, Zhang H, Marchand C. DNA topoisomerases and their poisoning by anticancer drugs. Chem Biol 2010; 17: 421-433.
3. Heisig P. Type II topoisomerases-Inhibitors, repair mechanmisms and mutations. Mutagenesis 2009; 24: 465-469.
4. Nitiss JL. Targeting DNA topoisomerase II in cancer chemotherapy. Nat Rev Cancer 2009; 9: 338-350.
5. Liu Z, Deibler RW, Chan HS, Zechiedrich L. The why and how of DNA unlinking. Nucleic acids Res 2009; 37: 661-671.
6. McClendon AK, Rodriguez AC, Osheroff N. Human topoisomerase IIα rapidly relaxes positively supercoiled DNA: Implications for enzyme action ahead of replication forks. J Biol Chem 2005; 280: 39337-39345.
7. Hsiang YH, Lihou MG, Liu LF. Arrest of replication forks by drug stabilized topoisomerase I-DNA cleavable complexes as a mechanism of cell killing by camptothecin. Cancer Res 1989; 49: 5077-5082.
8. Ju BG, Lunyak VV, Perissi V, Garcia-Bessets L, Rose DW, Glass CK, Rosenfeld MG. A topoisomerase IIβ-mediated dsDNA break required for regulation of transcription. Science 2006; 312: 1798-1802.
9. D'Arpa P, Beardmore C, Liu LF. Involvement of nucleic acid synthesis in cell killing mechanisms of topoisomerase poisons. Cancer Res 1990; 50: 6916-6924.
10. Wu J, Liu LF. Processing of topoisomerase I cleavable complexes into DNA by transcription. Nucleic Acids Res 1997; 25: 4181-4186.
11. Kastan MB. DNA damage responses: Mechanisms and roles in human disease. 2007 G.H.A. Cloves Memorial Award Lecture. Mol Cancer Res 2008; 6: 517-524.
12. Lovejoy CA, Cortez D. Common mechanisms of PIKK regulation. DNA Repair 2009; 8: 1004-1008.
13. Cuadrado M, Martinez-Pastor B, Fernandez-Capetillo O. ATR activation in response to ionizing radiation: still ATM territory. Cell Division 2006, 1, 7 Available at:.
14. Rogakou EP, Pilch DR, Orr AH, Ivanova VS, Bonner WM. DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139. J Biol Chem 1998; 273: 5858-5868.
15. 125IUdr-induced DNA double-strand breaks with γ-H2AX antibody. Radiat Res 2002; 158: 486-492.
16. Huang X, Traganos F, Darzynkiewicz Z. DNA damage induced by DNA topoisomerase I- and topoisomerase II- inhibitors detected by histone H2AX phosphorylation in relation to the cell cycle phase and apoptosis. Cell Cycle 2003; 2: 614-619.
17. Banath JP, Olive PL. Expression of phosphorylated histone H2AX as a surrogate of cell killing by drugs that create DNA double-strand breaks. Cancer Res 2003; 63: 4347-4350.
18. Huang X, Okafuji M, Traganos F., Luther E, Holden E, Darzynkiewicz Z. Assessment of histone H2AX phosphorylation induced by DNA topoisomerase I and II inhibitors topotecan and mitoxantrone and by DNA crosslinking agent cisplatin. Cytometry A 2004; 58A: 99-110.
19. Olive PL, Banath JP, Sinnott LT. Phosphorylated histone H2AX in spheroids, tumors, and tissues of mice exposed to etoposide and 3-amino-1, 2, 4-benzotriazine-1, 3-dioxide. Cancer Res 2004; 64: 5363-5369.
20. Kurose A, Tanaka T, Huang X, Halicka HD, Traganos F, Dai W, Darzynkiewicz Z. Assessment of ATM phosphorylation on Ser-1981 induced by DNA topoisomerase I and II inhibitors in relation to Ser-139-histone H2AX phosphorylation, cell cycle phase and apoptosis. Cytometry A 2005; 68A: 1-9.
21. Tanaka T, Kurose A, Huang X, Dai W, Darzynkiewicz Z. ATM kinase activation and histone H2AX phosphorylation as indicators of DNA damage by DNA topoisomerase I inhibitor topotecan and during apoptosis. Cell Prolif 2006; 39: 49-60.
22. Huang X, Kurose A, Tanaka T, Traganos F, Dai W, Darzynkiewicz Z. Activation of ATM and histone H2AX phosphorylation induced by mitoxantrone but not by topotecan is prevented by the antioxidant N-acetyl-L-cysteine. Cancer Biol Ther 2006; 5: 959-964.
23. Zhao H, Traganos F, Darzynkiewicz Z. Kinetics of histone H2AX phosphorylation and Chk2 activation in A549 cells treated with topotecan and mitoxantrone in relation to the cell cycle phase. Cytometry A 2008; 73A: 480-489.
24. Smart DJ, Halicka HD, Schmuck G, Traganos F, Darzynkiewicz Z, Williams GM. Assessment of DNA double-strand breaks and γH2AX induced by the topoisomerase II poisons etoposide and mitoxantrone. Mutat Res 2008; 641: 43-47.
25. Tanaka T, Halicka HD, Traganos F, Seiter K, Darzynkiewicz Z. Induction of ATM activation, histone H2AX phosphorylation and apoptosis by etoposide: Relation to the cell cycle phase. Cell Cycle 2007; 6: 371-376.
26. Salic A, Mitchison TJ. A chemical method for fast and sensitive detection of DNA synthesis in vivo. Proc Natl Acad Sci USA 2008; 105: 2415-2420.
27. Diermeier-Daucher S, Clarke ST, Hill D, Vollmann-Zwerenz A, Bradford JA, Brockhoff G. Cell type specific applicability of 5-ethynyl-2-deoxyuridine (EdU) for dynamic proliferation assessment in flow cytometry. Cytometry A 2009; 75A: 535-546.
28. Hamelik RA, Krishan A. Click-iT assay with improved DNA frequency histograms. Cytometry A 2009; 75: 862-865.
29. Darzynkiewicz Z, Traganos F, Zhao H, Halicka HD, Li J. Cytometry of DNA replication and RNA synthesis: Historical perspective and recent advances based on "click chemistry" Cytometry A 2011; 79A: 328-337.
30. Zhao H, Li J, Traganos F, Halicka HD, Zarebski M, Dobrucki J, Darzynkiewicz Z. Cell fixation in zinc salt solution (ZBF) is compatible with DNA damage response detection by phospho-specific antibodies. Cytometry A 2011; 79A: 470-476.
31. Zhao H, Traganos F, Darzynkiewicz Z, Kinetics of the UV-induced DNA damage response in relation to cell cycle phase. Correlation with DNA replication. Cytometry A 2010; 77A: 285-293.
32. Zhao H, Dobrucki J, Rybak P, Traganos F, Halicka HD, Darzynkiewicz Z. Induction of DNA damage signaling by oxidative stress in relation to DNA replication as detected using "click chemistry". Cytometry A 2011; 79A: 897-902.
33. Leonhardt H, Rahn HP, Weinzierl P, Sporbert A, Cremen T, Zink D, Cardoso MC. Dynamics of DNA replication factories in living cells. J Cell Biol 2000; 149: 271-279.
34. Zhao H, Traganos F, Darzynkiewicz Z. Phosphorylation of p53 on Ser15 during cell cycle caused by Topo I and Topo II inhibitors in relation to ATM and Chk2 activation. Cell Cycle 2008; 7: 3048-3055.
35. Del Bino G, Lassota P, Darzynkiewicz Z. The S-phase cytotoxicity of camptothecin. Exp Cell Res 1991; 193: 27-35.
36. Tanaka T, Halicka HD, Huang X, Traganos F, Darzynkiewicz Z. Constitutive histone H2AX phosphorylation and ATM activation, the reporters of DNA damage by endogenous oxidants. Cell Cycle 2006; 5: 1940-1945.
37. Tanaka T, Kajstura M, Halicka HD, Traganos F, Darzynkiewicz Z. Constitutive histone H2AX phosphorylation and ATM activation are strongly amplified during mitogenic stimulation of lymphocytes. Cell Prolif 2007; 40: 1-13.
38. Gorczyca W, Gong J, Ardelt B, Traganos F, Darzynkiewicz Z. The cell cycle related differences in susceptibility of HL-60 cells to apoptosis induced by various antitumor agents. Cancer Res 1993; 53: 3186-3192.
39. Kapuscinski J, Darzynkiewicz Z. Interactions of antitumor agents ametantrone and mitoxantrone (novantrone) with double-stranded DNA. Biochem Pharmacol 1985; 34: 4203-4213.
40. Bender RP, Jablonksy MJ, Shadid M, Romaine I, Dunlap N, Anklin C, Graves DE, Osheroff N. Substituents on etoposide that interact with human topoisomerase IIalpha in the binary enzyme-drug complex: Contributions to etoposide binding and activity. Biochemistry 2008; 47: 4501-4509.
41. Tanaka T, Huang X, Halicka HD, Zhao H, Traganos F, Albino AP, Dai W, Darzynkiewicz Z. Cytometry of ATM activation and histone H2AX phosphorylation to estimate extent of DNA damage induced by exogenous agents. Cytometry A 2007; 71A: 648-661.
42. Bolte S, Coldelieres FP. A guided tour into subcellular colocalization analysis in light microscopy. J Microsc 2006; 224: 213-232.
43. Abramoff MD, Magalhaes PJ, Ram SJ. Image processing with ImageJ. Biophoton Int 2004; 7: 36-42.
44. Manders EEM, Verbeek FJ, Aten JA. Measurement of co-localization on objects in dual confocal images. J Microsc 1993; 169: 375-382.
45. Halicka HD, Zhao H, Podhorecka M, Traganos F, Darzynkiewicz Z. Cytometric detection of chromatin relaxation, an early reporter of DNA damage response. Cell Cycle 2009; 8: 2233-2237.
46. Housman D, Huberman JA. Changes in the rate of DNA replication fork movement along the S phase in mammalian cells. J Mol Biol 1975; 94: 173-176.