An updated method for the isolation and culture of primary calf hepatocytes

Veterinary Journal

Volumn 191, Issue 3, 2012, Pages 323-326

  Zhang, Zhi Gang ^a   Li, Xiao Bing ^b   Gao, Li ^a   Liu, Guo Wen ^b   Kong, Tao ^c   Li, Yan Fei ^a   Wang, Hong Bin ^a   Zhang, Cai ^c   Wang, Zhe ^b   Zhang, Ri He ^b  

^a NORTHEAST AGRICULTURAL UNIVERSITY   (China)

^b JILIN UNIVERSITY   (China)

^c HENAN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Calf; Cell isolation; Hepatocyte function; Hepatocytes; Primary culture

Indexed keywords

ALBUMIN; COLLAGENASE; LACTATE DEHYDROGENASE; TRYPAN BLUE; UREA;

ANIMAL CELL; ARTICLE; CALF (BOVINE); CELL COUNT; CELL FUNCTION; CELL GROWTH; CELL ISOLATION; CELL SHAPE; CELL STRUCTURE; CELL SURVIVAL; CELL VIABILITY; CONTROLLED STUDY; LIVER CELL; LIVER CELL CULTURE; NEWBORN; NONHUMAN;

ALBUMINS; ANIMALS; CATTLE; CELL CULTURE TECHNIQUES; HEPATOCYTES; L-LACTATE DEHYDROGENASE; UREA;

BOVINAE;

EID: 83555169633     PISSN: 10900233     EISSN: 15322971     Source Type: Journal    
DOI: 10.1016/j.tvjl.2011.01.008     Document Type: Article

References (26)

1. Bakala A., Karlik W., Wiechetek M. Preparation of equine isolated hepatocytes. Toxicology In Vitro 2003, 17:615-621.
2. Caperna T.J., Shannon A.E., Poch S.M., Garrett W.M., Richards M.P. Hormonal regulation of leptin receptor expression in primary cultures of porcine hepatocytes. Domestic Animal Endocrinology 2005, 29:582-592.
3. Carvalho M., Remiao F., Milhazes N., Borges F., Fernandes E., Carvalho F., Bastos M.L. The toxicity of N-methyl-alphamethyldopamine to freshly isolated rat hepatocytes is prevented by ascorbic acid and N-acetylcysteine. Toxicology 2004, 200:193-203.
4. Cheng Y.B., Wang Y.J., Zhang S.C., Liu J., Chen Z., Li J.J. Response of porcine hepatocytes in primary culture to plasma from severe viral hepatitis patients. World Journal of Gastroenterology 2005, 11:7585-7590.
5. Clouet A.S., Le Bizec B., Boerlen F., Monteau F., André F. Calf primary hepatocyte culture as a tool for anabolic steroid metabolism studies. Analyst 1998, 123:2489-2492.
6. Clouet A.S., Le Bizec B., Le Pape M.A., Maume D., Monteau F., Andre F. Biosynthesis of 6β-hydroxymethyltestosterone using bovine hepatocyte cultures. The Journal of Steroid Biochemistry and Molecular Biology 2000, 74:57-62.
7. Donkin S.S., Armentano L.E. Preparation of extended in vitro cultures of bovine hepatocytes that are hormonally responsive. Journal of Animal Science 1993, 71:2218-2227.
8. Dvorak Z., Ulrichova J., Weyhenmeyer R., Simanek V. Cytotoxicity of colchicine derivatives in primary cultures of human hepatocytes. Biomedical Papers of the Medical Faculty of the University Palacký Olomouc Czech Republic 2007, 151:47-52.
9. Dvorak Z., Vrzal R., Štarha P., Klanicova A., Travnicek Z. Effects of dinuclear copper (II) complexes with 6-(benzylamino) purine derivatives on AhR and PXR dependent expression of cytochromes P450 CYP1A2 and CYP3A4 genes in primary cultures of human hepatocytes. Toxicology in Vitro 2010, 24:425-429.
10. El-Shenawy N.S. Effects of insecticides fenitrothion, endosulfan and abamectin on antioxidant parameters of isolated rat hepatocytes. Toxicology in Vitro 2010, 24:1148-1157.
11. Forsell J.H., Jesse B.W., Shull L.R. A technique for isolation of bovine hepatocytes. Journal of Animal Science 1985, 60:1597-1609.
12. Grajeda P., Ramírez M.V., de Mejía E.G. Vitamin C protects against in vitro cytotoxicity of cypermethrin in rat hepatocytes. Toxicology in Vitro 2004, 18:13-19.
13. Howard R.B., Pesch L.A. Preparation and partial characterization of intact isolated parenchymal cells from rat liver. Biology Chemistry 1968, 243:3105-3114.
14. Howard R.B., Christensen A.K., Gibbs F.A., Pesch L.A. The enzymatic preparation of isolated intact parenchymal cells from rat liver. The Journal of Cell Biology 1967, 35:675-684.
15. Ji Q.L., Shi X.L., Lin R., Mao Y.H., Zhai X.G., Lin Q.Q., Zhang J.Y. Participation of lipid transport and fatty acid metabolism in valproate sodium-induced hepatotoxicity in HepG2 cells. Toxicology in Vitro 2010, 24:1086-1091.
16. Kaltenbach J.P. Mechanical preparation of isolated hepatocytes. Experimental Cell Research 1954, 7:568-574.
17. Montesissa C., Capolongo F., Santi A., Biancotto G., Dacasto M. Metabolism of tilmicosin by rabbit liver microsomes and hepatocytes. The Veterinary Journal 2004, 167:87-94.
18. Müller A.S., Pallauf J. Effect of increasing selenite concent rations, vitamin E supplementation and different fetal calf serum content on GPx1 activity in primary cultured rabbit hepatocytes. Journal of Trace Element Medicine Biology 2003, 17:183-192.
19. Puviani A.C., Ottolenghi C., Tassinari B., Pazzi P., Morsiani E. An update on high-yield hepatocyte isolation methods and on the potential clinical use of isolated liver cells. Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology 1998, 121:99-109.
20. Rothuizen J. Canine chronic liver disease: where are we now? Four pointers for future research. The Veterinary Journal 2004, 167:219.
21. Sand T.E., Christoffersen T. A simple medium for the study of hepatocyte growth in culture under defined conditions. In Vitro Cellular and Developmental Biology 1988, 24:981-984.
22. Seglen P.O. Preparation of isolated rat liver cells. Methods in Cell Biology 1976, 13:29-34.
23. Spotorno V.G., Hidalgo A., Barbich M., Lorenti A., Zabal O. Culture of bovine hepatocytes: a non-perfusion technique for cell isolation. Cytotechnology 2006, 51:51-56.
24. Walpole H.E., Lee W.M., Walle T., Walle U.K., Wilson M.J., Kennedy J.W. Rabbit hepatocytes in primary culture: preparation, viability and use in studies of propranolol metabolism. Hepatology 1990, 11:394-400.
25. Wang C.C., Lin C.C., Young T.H., Chen Y.S., Yong C.C., Kobayashi E., Wu C.H., Yang C.H., Chen C.L., Hung K.C. Culture and characterization of human hepatocytes isolated from hepatitis C virus infected liver: effect of collagen-coated surface and FBS-supplemented medium. Biochemical Engineering Journal 2006, 29:149-156.
26. 2+ homeostasis. Physiological Research 2007, 56:193-201.