Culture of bovine hepatocytes: A non-perfusion technique for cell isolation

Cytotechnology

Volumn 51, Issue 2, 2006, Pages 51-56

  Spotorno, Viviana Graciela ^a   Hidalgo, Alejandra ^b   Barbich, Mariana ^b   Lorenti, Alicia ^b   Zabal, Osvaldo ^a  

^a INSTITUTO NACIONAL DE TECNOLOGÍA AGROPECUARIA INTA   (Argentina)

^b HOSPITAL ITALIANO DE BUENOS AIRES   (Argentina)

Author keywords

Bovine hepatocytes; Bovine liver; Cell culture; Cell isolation; Non perfusion; Purification of parenchymal cells

Indexed keywords

7 ETHOXYCOUMARIN; ACTIN; ALBUMIN; BIOLOGICAL MARKER; COLLAGENASE;

ANIMAL CELL; ARTICLE; CELL FUNCTION; CELL ISOLATION; CELL SEPARATION; CELL TYPE; CELL ULTRASTRUCTURE; CELL VIABILITY; CENTRIFUGATION; CONTROLLED STUDY; COW; CULTURE TECHNIQUE; IMMUNOCYTOCHEMISTRY; LIVER; LIVER CELL; LIVER CELL CULTURE; NONHUMAN; PARENCHYMA; PERFUSION; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; SOLUTION AND SOLUBILITY; STELLATE CELL;

BOS TAURUS;

EID: 33751098534     PISSN: 09209069     EISSN: 15730778     Source Type: Journal    
DOI: 10.1007/s10616-006-9000-0     Document Type: Article

References (15)

1. Alston-Smith J, Pertoft H (1995) Isolation of liver cells: a symtem for obtaining pure cells in monolayer cultures from a single rat liver. In: Doyle A, Griffiths JB, Newell DG (eds) Cell and tissue culture. Laboratory Procedures, John Wiley & Sons, New York, USA, pp 12B: 14.1-14.9
2. Berry MN, Friend DS (1969) High-yield preparation of isolated rat liver parenchymal cells. J Cell Biol 43:506-520
3. Berry MN, Edwards AM, Barritt GJ (1991) Isolated hepatocytes preparation, properties and applications. In: Burdon RH, van Knippenbergr PH (eds) Laboratory techniques in biochemistry and molecular biology, Elsevier Science Publishers, Amsterdam, The Netherlands
4. Bhatia SN, Balis UJ, Yarmush ML, Toner M (1998) Microfabrication of hepatocyte/fibroblast co-cultures: role of homotypic cell interactions. Biotechnol Prog 14:378-387
5. Donkin SS, Armentano LE (1993) Preparation of extended in vitro cultures of bovine hepatocytes that are hormonally responsive. J Anim Sci 71:2218-2227
6. Enat R, Jefferson DM, Ruiz Opazo N, Gatmaitan Z, Leinwand LA, Reid L (1984) Hepatocyte proliferation in vitro: its dependence on the use of serum-free hormonally defined medium and substrata of extracellular matrix. Proc Natl Acad Sci 81:1411-1415
7. Forsell JH, Jesse BW, Shull LR (1985) A technique for isolation of bovine hepatocytes. J Anim Sci 60:1597-1609
8. Fry JF, Jones CA, Wiebkin P, Bellemann P, Bridges JW (1976) The enzymic isolation of adult rat hepatocytes in a functional and viable state. Anal Chem 71:341-350
9. Funaki N, Tanaka J, Sugiyama T, Ohshio G, Nonaka A, Yotsumoto F, Sugie T, Imamura M (2002) Successive cultures of mature hepatocyte autotransplantation to assist liver function after liver resection for cancer. Oncol Reports 9:713-721
10. Geerts A, Niki T, Hellemans K, De Craemer D, Van Der Berg K, Lazou JM, Stange G, Van De Winkel M, De Bleser P (1998) Purification of hepatic stellate cells by side scatter-activated cell sorting. Hepatology 27(2):590-598
11. Gibson-D'Ambrosio RE, D'Ambrosio SM (1996) Replicative and Functional Cultures of Normal Human, Hepatocytes. In: Doyle A, Griffiths JB, Newell DG, (eds) Cell & Tissue Culture. Laboratory Procedures, John Wiley & Sons, New York, USA, pp 12B: 17.1-17.16
12. Lorenti A, Barbich M, Hidalgo A, Hyon SH, Sorroche P, Guinle A, Schenone A, Chamoles N, Argibay P (2001) Culture of porcine hepatocytes: the dogma of exogenous matrix revisited. Artificial Organs 25(7):546-550
13. Nakamura T, Tomita Y, Ichihara A (1983a) Density-dependent growth control of adult rat hepatocytes in primary culture. J Biochem 94:1029-1035
14. Nakamura T, Yoshimoto K, Nacayama Y, Tomita Y, Ichihara A (1983b) Reciprocal modulation of growth and differentiated functions of mature hepatocytes in primary culture by cell-cell contact and cell membrane. Proc Natl Acad Sci 80:7229-7233
15. Shull L, Kirsch DG, Lohse CL, Carlson GP, Doody LA, Wisniewski JA (1986) Xenobiotic metabolism in suspension and primary cultures of isolated hepatocytes prepared from caudate process of bovine liver. Am J Vet Res 49(9):2043-2052