Primary recovery of lipase derived from Burkholderia cenocepacia strain ST8 and recycling of phase components in an aqueous two-phase system

Biochemical Engineering Journal

Volumn 60, Issue , 2012, Pages 74-80

  Show, Pau Loke ^a   Tan, Chin Ping ^a   Anuar, Mohd Shamsul ^a   Ariff, Arbakariya ^a   Yusof, Yus Aniza ^a   Chen, Soo Kien ^a   Ling, Tau Chuan ^b  

^a UNIVERSITY PUTRA MALAYSIA   (Malaysia)

^b UNIVERSITY OF MALAYA   (Malaysia)

Author keywords

Aqueous two phase; Bioseparations; Enzymes; Lipase; Protein recovery; Purification

Indexed keywords

AQUEOUS TWO PHASE; AQUEOUS TWO PHASE SYSTEM; BIO SEPARATION; BURKHOLDERIA; CONCENTRATED SOLUTION; PHASE COMPONENT; PHOSPHATE SOLUTIONS; POTASSIUM PHOSPHATE; PRIMARY SYSTEMS; PROTEIN RECOVERY; PURIFICATION FACTORS; RANDOM COPOLYMER; SECONDARY SYSTEM; TIE-LINE; TWO PHASE SYSTEMS; VOLUME RATIO; WATER SOLUTIONS;

COPOLYMERS; ENZYMES; ETHYLENE; LIPASES; PHASE SEPARATION; POLYMERS; POTASSIUM; PROPYLENE; RECOVERY; RECYCLING; SECONDARY RECOVERY;

PURIFICATION;

COPOLYMER; ETHYLENE OXIDE; PHOSPHATE; POLYMER; POTASSIUM DIHYDROGEN PHOSPHATE; PROPYLENE OXIDE; TRIACYLGLYCEROL LIPASE; WATER;

ARTICLE; BACTERIAL STRAIN; BURKHOLDERIA CENOCEPACIA; NONHUMAN; PRIORITY JOURNAL; PURIFICATION; SOLUTION AND SOLUBILITY;

BURKHOLDERIA CENOCEPACIA;

EID: 83555165947     PISSN: 1369703X     EISSN: 1873295X     Source Type: Journal    
DOI: 10.1016/j.bej.2011.10.005     Document Type: Article

References (44)

1. Ghosh S., Vijayalakshmi R., Swaminathan T. Evaluation of an alternative source of dextran as a phase forming polymer for aqueous two-phase extractive system. Biochem. Eng. J. 2004, 21:241-252.
2. Rajeeva S., Lele S.S. Three-phase partitioning for concentration and purification of laccase produced by submerged cultures of Ganoderma sp. WR-1. Biochem. Eng. J. 2011, 54:103-110.
3. Pereira M., Wu Y.T., Venâncio A., Teixeira J. Aqueous two-phase extraction using thermoseparating polymer: a new system for the separation of endo-polygalacturonase. Biochem. Eng. J. 2003, 15:131-138.
4. Carlsson M., Berggren K., Linse P., Veide A., Tjerneld F. Effects of fused tryptophan rich peptides to a recombinant protein A domain on the partitioning in polyethylene glycol-dextran and Ucon-dextran aqueous two-phase systems. J. Chromatogr. A 1996, 756:107-117.
5. Alred P.A., Kozlowski A., Harris J.M., Tjerneld F. Application of temperature-induced phase partitioning at ambient temperature for enzyme purification. J. Chromatogr. A 1994, 659:289-298.
6. Johansson H.O., Karlström G., Tjerneld F. Temperature-induced phase partitioning of peptides in water solutions of ethylene oxide and propylene oxide random copolymers. Biochim. Biophys. Acta Gen. Subj. 1997, 1335:315-325.
7. Bevilaqua J.V., Pinto J.C., Lima L.M., Barreiro E.J., Alves T.L.M., Freire D.M.G. Enzymatic hydrolysis by immobilized lipase applied to a new prototype anti-asthma drug. Biochem. Eng. J. 2004, 21:103-110.
8. Chang Y.-K., Chen J.-P., Sheu J.-R., Cheng P.-J., Su C.-H., Chou S.-Y. Direct recovery of alcohol dehydrogenase from unclarified yeast cell homogenate by IDEBAC using an improved scheme for elution. Biochem. Eng. J. 2006, 30:1-10.
9. Romdhane I.B.-B., Fendri A., Gargouri Y., Gargouri A., Belghith H. A novel thermoactive and alkaline lipase from Talaromyces thermophilus fungus for use in laundry detergents. Biochem. Eng. J. 2010, 53:112-120.
10. Dembczynski R., Bialas W., Jankowski T. Recycling of phase components during lysozyme extraction from hen egg white in the EO50PO50/K2HPO4 aqueous two-phase system. Biochem. Eng. J. 2010, 51:24-31.
11. Johansson H.O., Persson J., Tjerneld F. Thermoseparating water/polymer system: a novel one-polymer aqueous two-phase system for protein purification. Biotechnol. Bioeng. 1999, 66:247-257.
12. Bouaziz A., Horchani H., Ben Salem N., Gargouri Y., Sayari A. Expression, purification of a novel alkaline Staphylococcus xylosus lipase acting at high temperature. Biochem. Eng. J. 2011, 54:93-102.
13. Chew Y.H., Chua L.S., Cheng K.K., Sarmidi M.R., Aziz R.A., Lee C.T. Kinetic study on the hydrolysis of palm olein using immobilized lipase. Biochem. Eng. J. 2008, 39:516-520.
14. Ogino H., Inoue S., Akagi R., Yasuda M., Doukyu N., Ishimi K. Refolding of a recombinant organic solvent-stable lipase, which is overexpressed and forms an inclusion body, and activation with lipase-specific foldase. Biochem. Eng. J. 2008, 40:507-511.
15. Tang Z.-X., Shi L.-E., Qian J.-Q. Neutral lipase from aqueous solutions on chitosan nano-particles. Biochem. Eng. J. 2007, 34:217-223.
16. Talukder M.M.R., Wu J.C., Fen N.M., Melissa Y.L.S. Two-step lipase catalysis for production of biodiesel. Biochem. Eng. J. 2010, 49:207-212.
17. Dabkowska K., Szewczyk K.W. Influence of temperature on the activity and enantioselectivity of Burkholderia cepacia lipase in the kinetic resolution of mandelic acid enantiomers. Biochem. Eng. J. 2009, 46:147-153.
18. McClean S., Callaghan M. Burkholderia cepacia complex: epithelial cell-pathogen confrontations and potential for therapeutic intervention. Med. Microbiol. J. 2009, 58:1-12.
19. Nagayama K., Spiess A.C., Büchs J. Gas phase enantioselective reduction catalyzed by immobilized ketoreductase: Effects of water activity and reaction temperature. Biochem. Eng. J. 2010, 52:301-303.
20. Doukyu N., Ogino H. Organic solvent-tolerant enzymes. Biochem. Eng. J. 2010, 48:270-282.
21. Guillén M., Benaiges M.D., Valero F. Comparison of the biochemical properties of a recombinant lipase extract from Rhizopus oryzae expressed in Pichia pastoris with a native extract. Biochem. Eng. J. 2011, 54:117-123.
22. Lau H.L., Ariff A., Woo K.K., Ling T.C., Hii S.L. Production and optimization of alkalostable lipase by alkalophilic Burkholderia cenocepacia ST8. Afr. J. Biotechnol. 2011, 10:7002-7009.
23. Ooi C.W., Hii S.L., Kamal S.M.M., Ariff A., Ling T.C. Extractive fermentation using aqueous two-phase systems for integrated production and purification of extracellular lipase derived from Burkholderia pseudomallei. Process Biochem. 2011, 46:68-73.
24. Ooi C.W., Tey B.T., Hii S.L., Ariff A., Wu H.S., Lan J.C.W., Juang R.S., Kamal S.M.M., Ling T.C. Direct purification of Burkholderia pseudomallei lipase from fermentation broth using aqueous two-phase systems. Biotechnol. Bioprocess Eng. 2009, 14:811-818.
25. Ooi C.W., Tey B.T., Hii S.L., Kamal S.M.M., Lan J.C.W., Ariff A., Ling T.C. Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems. Process Biochem. 2009, 44:1083-1087.
26. Show P.L., Tan C.P., Anuar M.S., Ariff A., Yusof Y.A., Chen S.K., Ling T.C. Direct recovery of lipase derived from Burkholderia cepacia in recycling aqueous two-phase flotation. Sep. Purif. Technol. 2011, 80:577-584.
27. Smith P.K., Krohn R.I., Hermanson G.T. Measurement of protein using bicinchoninic acid. Anal. Biochem. 1985, 150:76-85.
28. Albertsson P.A. Partition of Cell Particles and Macromolecules 1986, Wiley, New York.
29. Jiang B., Li Z.G., Dai J.Y., Zhang D.J., Xiu Z.L. Aqueous two-phase extraction of 2,3-butanediol from fermentation broths using an ethanol/phosphate system. Process Biochem. 2009, 44:112-117.
30. Persson J., Johansson H.O., Galaev I., Mattiasson B., Tjerneld F. Aqueous polymer two-phase systems formed by new thermoseparating polymers. Bioseparation 2000, 9:105-116.
31. Persson J., Johansson H.O., Tjerneld F. Purification of protein and recycling of polymers in a new aqueous two-phase system using two thermoseparating polymers. J. Chromatogr. A 1999, 864:31-48.
32. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685.
33. Li M., Peeples T.L. Purification of hyperthermophilic archaeal amylolytic enzyme (MJA1) using thermoseparating aqueous two-phase systems. J.Chromatogr. B: Anal. Technol. Biomed. Life Sci. 2004, 807:69-74.
34. Fero A.B.J., Jahim J.M. Application of the aqueous two-phase thermoseparating systems of Dehypon® LS 54-the waxy maize starch for protein extraction. J. Appl. Sci. 2010, 10:2596-2601.
35. Saravanan S., Rao J.R., Nair B.U., Ramasami T. Aqueous two-phase poly(ethylene glycol)-poly(acrylic acid) system for protein partitioning: Influence of molecular weight, pH and temperature. Process Biochem. 2008, 43:905-911.
36. Berggren K., Johansson H.O., Tjerneld F. Effects of salts and the surface hydrophobicity of proteins on partitioning in aqueous two-phase systems containing thermoseparating ethylene oxide-propylene oxide copolymers. J. Chromatogr. A 1995, 718:67-79.
37. Lima V.M.G., Krieger N., Mitchell D.A., Fontana J.D. Activity and stability of a crude lipase from Penicillium aurantiogriseum in aqueous media and organic solvents. Biochem. Eng. J. 2004, 18:65-71.
38. Ashipala O.K., He Q. Optimization of fibrinolytic enzyme production by Bacillus subtilis DC-2 in aqueous two-phase system (poly-ethylene glycol 4000 and sodium sulfate). Bioresour. Technol. 2008, 99:4112-4119.
39. Sinha R., Singh S.P., Ahmed S., Garg S.K. Partitioning of a Bacillus alkaline protease in aqueous two-phase systems. Bioresour. Technol. 1996, 55:163-166.
40. Paquet V., Myint M., Roque C., Soucaille P. Partitioning of pristinamycins in aqueous two-phase systems: A first step toward the development of antibiotic production by extractive fermentation. Biotechnol. Bioeng. 1994, 44:445-451.
41. Minuth T., Thömmes J., Kula M.R. A closed concept for purification of the membrane-bound cholesterol oxidase from Nocardia rhodochrous by surfactant-based cloud-point extraction, organic-solvent extraction and anion-exchange chromatography. Biotech. Appl. Biochem. 1996, 23:107-116.
42. Tasnádi G., Forró E., Fülöp F. Burkholderia cepacia lipase is an excellent enzyme for the enantioselective hydrolysis of β-heteroaryl-β-amino esters. Tetrahedron Asymmetry 2009, 20:1771-1777.
43. Kepka C., Collet E., Persson J., Ståhl Å., Lagerstedt T., Tjerneld F., Veide A. Pilot-scale extraction of an intracellular recombinant cutinase from E. coli cell homogenate using a thermoseparating aqueous two-phase system. J. Biotechnol. 2003, 103:165-181.
44. Persson J., Kaul A., Tjerneld F. Polymer recycling in aqueous two-phase extractions using thermoseparating ethylene oxide-propylene oxide copolymers. J. Chromatogr. B Biomed. Sci. Appl. 2000, 743:115-126.