Direct purification of Burkholderia Pseudomallei lipase from fermentation broth using aqueous two-phase systems

Biotechnology and Bioprocess Engineering

Volumn 14, Issue 6, 2009, Pages 811-818

  Ooi, Chien Wei ^a   Tey, Beng Ti ^a   Hii, Siew Ling ^b   Ariff, Arbakariya ^a   Wu, Ho Shing ^c   Lan, John Chi Wei ^c   Juang, Ruey Shin ^c   Kamal, Siti Mazlina Mustapa ^a   Ling, Tau Chuan ^a  

^a UNIVERSITY PUTRA MALAYSIA   (Malaysia)

^b UNIVERSITI TUNKU ABDUL RAHMAN   (Malaysia)

^c YUAN ZE UNIVERSITY   (Taiwan)

Author keywords

Aqueous two phase systems; Lipase; Polyethylene glycol; Purification; Recovery

Indexed keywords

AQUEOUS TWO PHASE; AQUEOUS TWO PHASE SYSTEM; AQUEOUS TWO-PHASE SYSTEMS; BURKHOLDERIA PSEUDOMALLEI; FERMENTATION BROTHS; HIGH YIELD; NEUTRAL SALTS; OPTIMUM CONDITIONS; PARTITION BEHAVIORS; POTASSIUM PHOSPHATE; PURIFICATION FACTORS; PURIFICATION PROCESS; TIE-LINE; VOLUME RATIO;

FERMENTATION; POLYETHYLENE OXIDES; PURIFICATION; SODIUM CHLORIDE; THERMOPLASTICS;

POLYETHYLENE GLYCOLS;

MACROGOL 6000; POTASSIUM DIHYDROGEN PHOSPHATE; SODIUM CHLORIDE; TRIACYLGLYCEROL LIPASE;

ANALYTICAL PARAMETERS; AQUEOUS SOLUTION; ARTICLE; BIOPROCESS; BURKHOLDERIA PSEUDOMALLEI; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME PURIFICATION; FERMENTATION; NONHUMAN; PH;

BURKHOLDERIA PSEUDOMALLEI;

EID: 77149155962     PISSN: 12268372     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12257-008-0306-y     Document Type: Article

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