Expression, purification of a novel alkaline Staphylococcus xylosus lipase acting at high temperature

Biochemical Engineering Journal

Volumn 54, Issue 2, 2011, Pages 93-102

  Bouaziz, Ahlem ^a   Horchani, Habib ^a   Ben Salem, Nadia ^a   Gargouri, Youssef ^a   Sayari, Adel ^a  

^a UNIVERSITY OF SFAX   (Tunisia)

Author keywords

Expression; Purification; Sequencing; Staphylococcus xylosus lipase; Synthesis; Thermo alkaline

Indexed keywords

EXPRESSION; SEQUENCING; STAPHYLOCOCCUS XYLOSUS LIPASE; SYNTHESIS; THERMO-ALKALINE;

AFFINITY CHROMATOGRAPHY; ALKALINITY; AMINO ACIDS; EMULSIFICATION; EMULSIONS; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE ENCODING; HYDROLASES; ORGANIC SOLVENTS; PURIFICATION; VEGETABLE OILS;

LIPASES;

BACTERIAL ENZYME; OLIVE OIL; STAPHYLOCCUS XYLOSUS LIPASE; TRIACYLGLYCEROL LIPASE; TRIBUTYRIN; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL STRAIN; ENZYME ACTIVITY; ENZYME PURIFICATION; ESCHERICHIA COLI; ESTERIFICATION; GENETIC CODE; HIGH TEMPERATURE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PRIORITY JOURNAL; PROTEIN EXPRESSION; THERMOSTABILITY;

ESCHERICHIA COLI; STAPHYLOCOCCUS XYLOSUS;

EID: 79952816834     PISSN: 1369703X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bej.2011.02.003     Document Type: Article

References (35)

1. Jaeger K.E., Reetz T.M. Microbial lipases from versatile tools for biotechnology. TIBTECH 1998, 16:396-403.
2. Macrae A.R., Hammond R.C. Present and future applications of lipases. Biotechnol. Genet. Eng. Rev. 1985, 3:193-217.
3. Roustan J.L., Chu A.R., Moulin G., Bigey F. A novel lipase/acyltransferase from the yeast Candida albicans: expression and characterisation of the recombinant enzyme. Appl. Microbiol. Biot. 2005, 68:203-212.
4. Rosenstein R., Götz F. Staphylococcal lipases: biochemical and molecular characterization. Biochimie 2000, 82:1005-1014.
5. Gupta R., Gupta N., Rathi P. Bacterial lipases: an overview of production, purification and biochemical properties. Appl. Microbiol. Biot. 2004, 64:763-781.
6. Lumor S.E., Akoh C.C. Esterification and hydrolytic activities of Candida rugosa lipase isoform 1 (LIP1) immobilized on Celite 545, Duolite A7, and Sephadex G-25. J. Agric. Food Chem. 2008, 56:10396-10398.
7. Chakraborty K., Raj R.P. An extra-cellular alkaline metallolipase from Bacillus licheniformis MTCC 6824: purification and biochemical characterization. Food Chem. 2008, 109:727-736.
8. Hasan F., Shah A.A., Hameed A. Industrial applications of microbial lipases. Enzyme Microb. Technol. 2006, 39:235-251.
9. Ha S.H., Lan M.N., Lee S.H., Hwang S.M., Koo Y.M. Lipase-catalyzed biodiesel production from soybean oil in ionic liquids. Microb. Technol. 2007, 41:480-483.
10. Yao H., Yu S., Zhang L., Zuo K., Ling H., Zhang F., Tang K. Isolation of a novel lipase gene from Serratia liquefaciens S33DB-1, functional expression in Pichia pastoris and its properties. Mol. Biotechnol. 2008, 38:99-107.
11. Quintana-Castro R., Di{dotless}az P., Gerardo Valerio-Alfaro G., Hugo S., Garci{dotless}a H.S., Rosamari{dotless}a Oliart-Ros R. Gene cloning, expression, and characterization of the Geobacillus thermoleovorans CCR11 thermoalkaliphilic lipase. Mol. Biotechnol. 2009, 42:75-83.
12. Jiang Z., Zheng Y., Luo Y., Wang G., Wang W., Ma Y., Wei D. Cloning and expression of a novel lipase gene from Pseudomonas fluorescens B52. Mol. Biotechnol. 2005, 31:95-101.
13. Lui B., Qian X., Wu Q., Lin X. Two lipase-catalyzed sequential synthesis of drug derivatives in organic media. Enzyme Microb. Technol. 2008, 43:375-380.
14. Horchani H., Mosbah H., Ben Salem N., Gargouri Y., Sayari A. Biochemical and molecular characterisation of a thermoactive, alkaline and detergent-stable lipase from a newly isolated Staphylococcus aureus strain. J. Mol. Catal. B: Enzym. 2009, 56:237-245.
15. Horchani H., Chaabouni M., Gargouri Y., Sayari A. Solvent-free lipase-catalyzed synthesis of long-chain starch esters using microwave heating: optimization by response surface methodology. Carbohyd. Polym. 2010, 79:194-201.
16. Horchani H., Ben Salem N., Zarai Z., Sayari A., Gargouri Y., Chaabouni M. Enzymatic synthesis of eugenol benzoate by immobilized Staphylococcus aureus lipase: optimization using response surface methodology and determination of antioxidant activity. Bioresour. Technol. 2010, 101:2809-2817.
17. Bouaziz A., Horchani H., Ben Salem N., Chaabouni M., Gargouri Y., Sayari S. Enzymatic propyl gallate synthesis in solvent-free system: optimization by response surface methodology. J. Mol. Catal. B: Enzym. 2010, 67:242-250.
18. Rathelot J., Julien R., Canioni P., Coeroli C., Sarda L. Studies on the effect of bile salt and colipase on enzymatic lipolysis. Improved method for the determination of pancreatic lipase and colipase. Biochimie 1975, 57:1117-1122.
19. Birnboim A. A rapid alkaline extraction method for the isolation of plasmid DNA. Method Enzymol. 1983, 100:243-255.
20. Sayari A., Agrebi N., Jaoua S., Gargouri Y. Biochemical and molecular characterization of staphylococcus simulans lipases. Biochimie 2001, 83:863-871.
21. Laemmli U.K. Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685.
22. Bradford M.M. A rapid and sensitive method for the quantitation of quantities of protein utilising the principle of protein-dye binding. Anal. Biochem. 1976, 72:248-254.
23. Bergman H., Jörnvall T. Electroblotting of individual polypeptides from SDS/polyacrylamide gels for direct sequence analysis. Eur. J. Biochem. 1987, 169:9-12.
24. Mosbah H., Sayari A., Mejdoub H., Dhouib H., Gargouri Y. Biochemical and molecular characterization of Staphylococcus xylosus lipase. Biochim. Biophys. Acta 2005, 1723:282-291.
25. Simons J.W., Adams H., Cox R.C., Dekker N., Götz F., Slotboom A.J., Verheij H.M. The lipase from Staphylococcus aureus. Expression in Escherichia coli, large-scale purification and comparison of substrate specificity to Staphylococcus hyicus lipase. Eur. J. Biochem. 1996, 242:760-769.
26. Mosbah H., Horchani H., Sayari A. The insertion of (LK) residues at the N-terminus of Staphylococcus xylosus lipase affects its catalytic properties and its enantioselectivity. Process Biochem. 2010, 45:777-785.
27. Jaeger S., Demleitner G., Götz F. Lipase of Staphylococcus hyicus: analysis of the catalytic triad by site-directed mutagenesis. FEMS Microbiol. Lett. 1992, 79:249-254.
28. Horchani H., Ouertani S., Gargouri Y., Sayari A. The N-terminal His-tag and the recombination process affect the biochemical properties of Staphylococcus aureus lipase produced in Escherichia coli. J. Mol. Catal. B: Enzym. 2009, 61:194-201.
29. Horchani H., Sabrina L., Régine L., Sayari A., Gargouri Y., Verger R. Heterologous expression and N-terminal His-tagging processes affect the catalytic properties of staphylococcal lipases: a monolayer study. J. Colloid Interface Sci. 2010, 350:586-594.
30. Chen S.J., Cheng C.Y., Chen T.L. Production of an alkaline lipase by Acinetobacter radioresistens. J. Ferment. Bioeng. 1998, 86:308-312.
31. Haki G.D., Rakshit S.K. Developments in industrially important thermostable enzymes: a review. Bioresour. Technol. 2003, 89:17-34.
32. Illanes A. Stability of biocatalysts. Electron. J. Biotechnol. 1999, 2:7-15.
33. Gargouri Y., Julien R., Bois A.G., Verger R., Sarda L. Studies on the detergent inhibition of pancreatic lipase activity. J. Lipid Res. 1983, 24:1336-1342.
34. Noble M.E.M., Cleasby A., Johnson L.N., Egmond M.R., Frenken L.G.J. FEBS Lett. 1993, 331:123-128.
35. Simons J.W., van Kampen M.D., Ubarretxena-Belandia I., Cox R.C., Alves dos Santos C.M., Egmond M.R., Verheij H.M. Biochemistry 1999, 38:2-10.