Refolding of a recombinant organic solvent-stable lipase, which is overexpressed and forms an inclusion body, and activation with lipase-specific foldase

Biochemical Engineering Journal

Volumn 40, Issue 3, 2008, Pages 507-511

  Ogino, Hiroyasu ^a   Inoue, Sosuke ^a   Akagi, Rieko ^a   Yasuda, Masahiro ^a   Doukyu, Noriyuki ^b   Ishimi, Kosaku ^a  

^a Osaka Prefecture University   (Japan)

^b TOYO UNIVERSITY   (Japan)

Author keywords

Chaperonins; Lipase; Organic solvent stability; Protein denaturation; Pseudomonas aeruginosa; Refolding

Indexed keywords

CHEMICAL ACTIVATION; ENZYME ACTIVITY; ESCHERICHIA COLI; ORGANIC SOLVENTS; PROTEIN FOLDING;

CHAPERONINS; ORGANIC SOLVENT STABILITY; PROTEIN DENATURATION; PSEUDOMONAS AERUGINOSA; REFOLDING;

ENZYME KINETICS;

CHAPERONIN; ENZYME; FOLDASE; ORGANIC SOLVENT; TRIACYLGLYCEROL LIPASE;

ARTICLE; CELL INCLUSION; CONFORMATIONAL TRANSITION; ENZYME ACTIVATION; ENZYME CONFORMATION; ENZYME DENATURATION; ENZYME PURIFICATION; ENZYME STABILITY; ESCHERICHIA COLI; GENE OVEREXPRESSION; NONHUMAN; PRIORITY JOURNAL; PROTEIN FOLDING; PSEUDOMONAS AERUGINOSA;

ESCHERICHIA COLI; PSEUDOMONAS AERUGINOSA;

EID: 44449161817     PISSN: 1369703X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bej.2008.01.022     Document Type: Article

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