Purification and characterization of a protease produced by Bacillus megaterium RRM2: Application in detergent and dehairing industries

Journal of Basic Microbiology

Volumn 51, Issue 6, 2011, Pages 614-624

  Rajkumar, Renganathan ^a   Jayappriyan, Kothilmozhian Ranishree ^a   Rengasamy, Ramasamy ^a  

^a UNIVERSITY OF MADRAS   (India)

Author keywords

Alkaline serine protease; Bacillus megaterium; Blood stain; Dehairing; Purification

Indexed keywords

BACTERIAL DNA; DETERGENT; ENZYME ACTIVATOR; ENZYME INHIBITOR; METAL; RIBOSOME DNA; RNA 16S; SERINE PROTEINASE;

ARTICLE; BACILLUS MEGATERIUM; BACTERIUM IDENTIFICATION; BIOTECHNOLOGY; CHEMISTRY; CLASSIFICATION; CLUSTER ANALYSIS; DNA SEQUENCE; ENZYME STABILITY; ENZYMOLOGY; FRACTIONATION; GENETICS; HAIR; ISOLATION AND PURIFICATION; LIQUID CHROMATOGRAPHY; METABOLISM; METHODOLOGY; MICROBIOLOGY; MOLECULAR GENETICS; MOLECULAR WEIGHT; NUCLEOTIDE SEQUENCE; PH; PHYLOGENY; RED ALGA; TEMPERATURE;

BACILLUS MEGATERIUM; BACTERIAL TYPING TECHNIQUES; BIOTECHNOLOGY; CHEMICAL FRACTIONATION; CHROMATOGRAPHY, LIQUID; CLUSTER ANALYSIS; DETERGENTS; DNA, BACTERIAL; DNA, RIBOSOMAL; ENZYME ACTIVATORS; ENZYME INHIBITORS; ENZYME STABILITY; HAIR; HYDROGEN-ION CONCENTRATION; METALS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PHYLOGENY; RHODOPHYTA; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA; SERINE PROTEASES; TEMPERATURE;

EID: 83155190422     PISSN: 0233111X     EISSN: 15214028     Source Type: Journal    
DOI: 10.1002/jobm.201000517     Document Type: Article

References (38)

1. Abidi, F., Limam, F., Nejib, M.M., 2008. Production of alkaline proteases by Botrytis cinerea using economic raw materials: Assay as biodetergent. Process Biochem., 43, 1202-1208.
2. Mitidieri, S., Martinelli, A.H.S., Schrank, A., Vainstein, M.H., 2006. Enzymatic detergent formulation containing amylase from Aspergillus niger: A comparative study with commercial detergent formulations. Bioresour. Technol., 97, 1217-1224.
3. Outtrup, H., 1990. Boyee Col. Microbial proteases and biotechnology. In: Fogarty, W.M., Kelly, C.T. (eds.), Microbial Enzymes and Biotechnology. Elsevier Science, New York, pp. 227-254.
4. Manachini, P.L., Fortina, M.G., 1998. Production in seawater of thermostable alkaline protease by a halotolerant strain of Bacillus licheniformis. Biotechnol Lett., 20, 565-568.
5. Mukherjee, A.K., Adhikari, H., Rai, S.K., 2008. Production of alkaline protease by a thermophilic Bacillus subtilis under solid-state fermentation (SSF) condition using Imperata cylindrica grass and potato peel as low-cost medium: Characterization and application of enzyme in detergent formulation. Biochem. Engin. J., 39, 353-361.
6. Bhaskar, N., Sudeepa, E.S., Rashmi, H.N., Tamil Selvi, A., 2007. Partial purification and characterization of protease of Bacillus proteolyticus CFR3001 isolated from fish processing waste and its antibacterial activities. Bioresour. Technol., 98, 2758-2764.
7. Doddapaneni, K.K., Tatineni, R., Vellanki, R.N., Gandu, B. et al., 2007. Purification and characterization of two novel extracellular proteases from Serratia rubidaea. Process Biochem., 42, 1229-1236.
8. Thanikaivelan, P., Rao, J.R., Nair, B.U., Ramasami, T., 2004. Progress and recent trends in biotechnological methods for leather processing. Trends Biotechnol., 22, 181-188.
9. Huang, Q., Peng, Y., Li, X., Wang, H.F. et al., 2003. Purification and characterization of an extracellular alkaline serine protease with dehairing function from Bacillus pumilus. Curr. Microbiol., 46, 169-173.
10. Wang, H.Y., Liu, D.M., Liu, Y., Cheng, C.F. et al., 2007. Screening and mutagenesis of a novel Bacillus pumilus strain producing alkaline protease for dehairing. Lett. App. Microbiol., 44, 1-6.
11. th ed., Baltimore: Williams and Wilkins.
12. Marchesi, J.R., Sato, T., Andrew, J.W., Martin, T.A. et al., 1998. Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA. Appl. Environ. Microbiol., 64, 795-799.
13. Rajkumar, R., Jayappriyan, K.R., Ramesh Kannan, P., Rengasamy, R., 2010. Optimization of culture conditions for the production of protease from Bacillus megaterium. Journal of Ecobiotechnol., 2/4, 40-46.
14. Kanekar, P.P., Nilegaonkar, S.S., Sarnaik, S.S., Kelkar A.S., 2002. Optimization of protease activity of alkaliphilic bacteria isolated from an alkaline lake in India. Bioresour. Technol., 85, 87-93.
15. Bradford, M.M., 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem., 72, 248-254.
16. Laemmli, U.K., 1970. Cleavage of structural proteins during assembly of head of bacteriophage T4. Nature., 227, 680-685.
17. Blum, H., Beier, H., Grose, H.J., 1987. Improver silver staining of plant protein, RNA and DNA in polyacrylamide gel. Electrophoresis., 8, 647-653.
18. Garcia-Carreno, F.L., Dimes, L.F., Haard, N.F., 1993. Substrate-gel electrophoresis for composition and molecular weight of proteinase or proteinaceous proteinase inhibitors. Anal. Biochem., 214, 65-69.
19. Woessner, J.F., 1961. The determination of hydroxyproline in tissue and protein samples containing small portions of this amino acids. Arch. Biochem. Biophys., 93, 440-447.
20. Official Methods of Analysis Sec. 1. 1965. Society of Leather Trades Chemists, Redbourn, Herts, UK.
21. Nirmala, M., 1999. Studies on the bacterial flora associated with some marine green algae. Doctoral Thesis. University of Madras, India.
22. Musa, N., Wei, S.W., 2008. Bacteria Attached on culture seaweed Gracilaria changii at Mengabang Telipot, Terengganu. Acad. J. Plant Scie., 1, 1-4.
23. Hadj-Ali, N.E., Agrebi, R., Ghorbel-Frikha, B., Sellami-Kamoun, A. et al., 2007. Biochemical and molecular characterization of detergent stable alkaline serine protease from a newly Bacillus lichenniformis NH1. Enzyme Microbiol. Technol., 40, 515-523.
24. Nilegaonkar, S.S., Zambare, V.P., Kanekar, P.P., Dhakephalkar, P.K. et al., 2007. Production and partial characterization of dehairing protease from Bacillus cereus MCM B-326. Bioresour. Technol., 98, 1238-1245.
25. Yossan, S., Reungsang, A., Yasuda, M., 2006. Purification and characterization of alkaline protease from Bacillus megaterium isolated from Thai fish sauce fermentation process. Science Asia., 32, 377-383.
26. Haddar, A., Agrebi, R., Bougatef, A., Hmidet, N. et al., 2009. Two detergent stable alkaline serine-proteases from Bacillus mojavensis A21: Purification, characterization and potential application as a laundry detergent additive. Bioresour. Technol., 100, 3366-3373.
27. Hmidet, N., El-Hadj Ali, N., Haddar, A., Kanoun, S. et al., 2009. Alkaline proteases and thermostable amylase co-produced by Bacillus licheniformis NH1: Characterization and potential application as detergent additive. Biochemical Engineering Journal., 47, 71-79.
28. Rao, C.S., Sathish, T., Ravichandra, P., Prakasham, R.S., 2009. Characterization of thermo and detergent stable serine protease from isolated Bacillus circulans and evaluation of eco-friendly applications. Process Biochem., 44, 262-268.
29. Beg, Q.K., Saxena, R.K., Gupta, R., 2002. Kinetic constants determination for an alkaline protease from Bacillus mojavensis using response surface methodology. Biotechnol. Bioeng., 78, 289-295.
30. Venugopal, M., Saramma, A.V., 2006. Characterization of alkaline protease from Vibrio fleevialis strain VM10 isolated from a mangrove sediment sample and its application as a laundry detergent additive. Proc. Biochem., 41, 1239-1243.
31. Donaghy, J.A., McKay, A.M., 1993. Production and properties of an alkaline protease by Aureobasidium pullulans. J. Appl. Bacteriol., 74, 662-666.
32. Tsuchide, O., Yamaga, Y., Ishizuka, T., Arai, T. et al., 1995. An alkaline protease from alkalophilic Bacillus sp. KSM-K16. Appl. Microbiol. Biotechnol., 43, 473-481.
33. Oberoi, R., Beg, Q.K., Puri, S., Saxena, R.K. et al., 2001. Characterization and wash performance analysis of an SDS-resistant alkaline protease from a Bacillus sp. W. J. Microbiol. Biotechnol., 17, 493-497.
34. Rao, M.B., Tanksale, A.M., Ghatge, M.S., Deshpande, V.V., 1998. Molecular and biotechnological aspects of microbial proteases. Microbiol Mol. Biol. Rev., 62, 597-635.
35. Dayanandan, A., Kanagraj, J., Sounderraj, L., Govindaraju, R. et al., 2003. Application of an alkaline protease in leather processing: an eco-friendly approach. J. Clean. Product, 11, 533-536.
36. Tiwary, E., Gupta, R., 2010. Medium optimization for a novel 58 kDa dimeric keratinase from Bacillus licheniformis ER-15: Biochemical characterization and application in feather degradation and dehairing of hides. Bioresour. Technol., 101, 6103-6110.
37. Annapurna, R.A., Chandrababu, N.K., Samivelu, N., Rose, C. et al., 1996. Eco-friendly enzymatic dehairing using extracellular protease from Bacillus species isolate. J. Am. Leath. Chem. Assoc., 91, 115-119.
38. Sivasubramanian, S., Murali Manohar, B., Rajaram, A., Puvanakrishnan, R., 2008. Ecofriendly lime and sulfide free enzymatic dehairing of skins and hides using a bacterial alkaline protease. Chemosphere., 70, 1015-1024.