Purification and characterization of two novel extra cellular proteases from Serratia rubidaea

Process Biochemistry

Volumn 42, Issue 8, 2007, Pages 1229-1236

  Doddapaneni, Kiran Kumar ^a   Tatineni, Radhika ^a   Vellanki, Ravi Nagaraj ^a   Gandu, Bharat ^a   Panyala, Nagender Reddy ^a   Chakali, Balumaddileti ^a   Mangamoori, Lakshmi Narasu ^a  

^a JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY   (India)

Author keywords

Detergent activity; Gel permeation chromatography; Metalloprotease; Serratia rubidaea; Solvent tolerance; Surface layer protein

Indexed keywords

DETERGENT ACTIVITY; METALLOPROTEASE; SERRATIA RUBIDAEA; SOLVENT TOLERANCE; SURFACE LAYER PROTEIN; ZYMOGRAPHY;

BACTERIA; BIOCHEMISTRY; GENES; MOLECULAR MASS; RNA; SURFACE ACTIVE AGENTS; SYNTHESIS (CHEMICAL);

PROTEINS;

BACTERIA (MICROORGANISMS); SERRATIA; SERRATIA RUBIDAEA; SERRATIA SP.;

EID: 34447621946     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2007.05.019     Document Type: Article

References (32)

1. Rao M.B., Tanksale A.M., Ghatge M.S., and Deshpande V.V. Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62 (1998) 597-635
2. Kumar C.G., and Hiroshi T. Microbial alkaline proteases: from a bioindustrial viewpoint. Biotechnol Adv 17 (1999) 561-594
3. Horikoshi K. Alkalophiles-from an industrial point of view. FEMS Microbiol Rev 18 (1996) 259-270
4. Shikha, Adhyayan S., and Nandan S.D. Improved production of alkaline protease from a mutant of alkalophilic Bacillus pantotheneticus using molasses as a substrate. Bioresour Technol 98 (2007) 881-885
5. Genckal H., and Tari C. Alkaline protease production from alkalophilic Bacillus sp. isolated from natural habitats. Enzyme Microb Technol 39 (2006) 703-710
6. Ravi C.P., Sudhakar Ch, and Annapurna J. Alkaline protease production by submerged fermentation in stirred tank reactor using Bacillus licheniformis NCIM-2042: effect of aeration and agitation regimes. Biochem Eng J 34 (2007) 185-192
7. Sana B., Ghosh D., Saha M., and Mukherjee J. Purification and characterization of a salt, solvent, detergent and bleach tolerant protease from a new gamma-proteobacterium isolated from the marine environment of the Sundarbans. Process Biochem 41 (2006) 208-215
8. Raja N.Z., Lee P.G., Mahiran B., and Abu B.S. An organic solvent stable alkaline protease from Pseudomonas aeruginosa strain enzyme purification and characterization. Enzyme Microb Technol 39 (2006) 1484-1491
9. Gupta A., Roy I., Khare S.K., and Gupta M.N. Purification and characterization of a solvent stable protease from Pseudomonas aeruginosa PseA. J Chromatogr A. 1069 (2005) 155-161
10. San L.W., Yi H.C., Yue H.Y., and Chuan L.W. Two novel surfactant-stable alkaline proteases from Vibrio fluvialis TKU005 and their applications. Enzyme Microb Technol 40 (2006) 1213-1220
11. Romero F.J., Luis A., Salas G.J.A., Díaz M., and Quirós L.M. Production, purification and partial characterization of two extracellular proteases from Serratia marcescens grown in whey. Process Biochem 36 (2001) 507-515
12. In: Seneath P.H.A., Mair N.S., Sharpe E.M., and Holt J.G. (Eds). Bergey's manual of systematic bacteriology. 9th ed. (1986), Williams and Wilkins, Baltimore
13. Ausubel F.M., Brent R., Kingston R.E., Moore D.D., Seidman J.G., Smith J.A., and Struhl K. Current protocols in molecular biology (1992), Greene Publishing Association, Wiley-Interscience, New York
14. Marchesi J.R., Sato T., Andrew J.W., Martin T.A., Fry J.C., Hiom S.J., and Wade W.G. Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA. Appl Environ Microbiol 64 (1998) 795-799
15. Kunitz M. Crytalline soybean trypsin inhibitor. II. General properties. J Gen Physiol 30 (1947) 291-310
16. Lowry O.H., Rosebrough N.J., Farr A.L., and Rondal R.L. Protein measurement with the Folin phenol reagent. J Biol Chem 193 (1951) 265-273
17. Laemmli U.K. Cleavage of structural proteins during assembly of head of bacteriophage T4. Nature 227 (1970) 680-685
18. Morrissey J.H. Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Anal Biochem 117 (1981) 307-310
19. Heussen C., and Dowdel E.B. Electrophoretic analysis of plasminogen activators in polyacrylamide gels containingsodium dodecyl sulfate and copolymerized substrates. Anal Biochem 102 (1980) 196-202
20. Robertson E.F., Kathleen D.H., Peter J.M., and Henry C.R. Rapid isoelectric focusing in a vertical polyacrylamide minigel system. Anal Biochem 167 (1987) 290-294
21. Anshu G., Ipsita R., Khare S.K., and Gupta M.N. Purification and characterization of a solvent stable protease from Pseudomonas aeruginosa PseA. J Chromatogr A 1069 (2005) 155-161
22. Mikhailova A.G., Likhareva V.V., Khairullin R.F., Lubenets N.L., Rumsh L.D., Demidyuk I.V., and Kostrov S.V. Psychrophilic trypsin-type protease from Serratia proteamaculans. Biochemistry (Mosc) 71 (2006) 563-570
23. Longo M.A., Novella I.S., Garcia L.A., and Diaz M. Comparison of Bacillus subtilis and Serratia marcescens as protease producers under different operating conditions. J Biosci Bioeng 88 (1999) 35-40
24. Beg Q.K., and Gupta R. Purification and characterization of an oxidationstable, thiol-dependent serine alkaline protease from Bacillus mojavensis. Enzyme Microb Technol 32 (2003) 294-304
25. Sinha N., and Satyanarayana T. Alkaline protease production by thermophilic Bacillus licheniformis. Indian J Microbiol 31 (1991) 425-430
26. Seong C.N., Jo J.S., Choi S.K., Kim S.W., Kim S.J., and Lee O.H. Production, purification and characterization of a novel thermo stable serine protease from soil isolate, Streptomyces tendae. Biotechnol Lett 26 (2004) 907-909
27. Chengfang M., and Siaolu J. A novel surfactant and oxidation stable alkaline protease from Vibrio merschnikovii DL 33-51. Process Biochem 40 (2005) 2167-2172
28. Klingeberg M., Galunsky B., Sjohom C., Kasche V., and Antranikian G. Purification and properties of a highly thermostable, sodium dodecyl sulfate-resistant and stereo specific protease from the extremely thermophilic archaeon Thermococcus stetteri. Appl Environ Microbiol 61 (1995) 3098-3104
29. Kawai E., Akatsuka H., Idei A., Shibatani T., and Omori K. Serratia marcescens slayer protein is secreted extracellularly via an ATP-binding cassette exporter, the lip system. Mol Microbiol 27 (1998) 941-952
30. Oberoi R., Beg Q.K., Puri S., Saxena R.K., and Gupta R. Characterization and wash performance analysis of an SDS-stable alkaline protease from a Bacillus sp. World J Microbiol Biotechnol 5 (2001) 493-497
31. Deane S.M., Robb F.T., and Woods D.R. Production and activation of SDS resistant alkaline serine exoprotease of Vibrio alginolyticus. J Gen Microbiol 133 (1987) 272-294
32. Venugopal M., and Saramma A.V. Characterization of alkaline protease from Vibrio fluvialis strain VM10 isolated from a mangrove sediment sample and its application as a laundry detergent additive. Process Biochem 41 (2006) 1239-1243