Preparation of anti-NYD-SP8 rabbit polyclonal antibody and its application in the analysis of NYD-SP8 expression in nasopharyngeal carcinoma cell lines and clinical tissues

Tumori

Volumn 97, Issue 5, 2011, Pages 655-659

  Yang, Ling Lin ^a,b   Wu, Jing Bo ^b   Liu, Zhi Gang ^a   Liu, Li ^a   Zeng, Mu Sheng ^a   Yan, Shan Shan ^a   Chan, Hsiao Chang ^c   Xia, Yun Fei ^a  

^a SUN YAT SEN UNIVERSITY CANCER CENTER   (China)

^b Luzhou Medical College   (Italy)

^c CHINESE UNIVERSITY OF HONG KONG   (Hong Kong)

Author keywords

Antibody preparation; Nasopharyngeal carcinoma; NYD SP8; uPAR

Indexed keywords

POLYCLONAL ANTIBODY; POLYCLONAL ANTIBODY NYD SP8; PROTEIN NYD SP8; UNCLASSIFIED DRUG; UROKINASE RECEPTOR;

ANTIBODY TITER; ARTICLE; CANCER CELL CULTURE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE OVEREXPRESSION; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; NASOPHARYNX CARCINOMA; NUCLEOTIDE SEQUENCE; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; TISSUE DISTRIBUTION; WESTERN BLOTTING;

EID: 83055193665     PISSN: 03008916     EISSN: 20382529     Source Type: Journal    
DOI: 10.1177/030089161109700519     Document Type: Article

References (23)

1. The MGC Project Team: The status, quality, and expansion of the NIH full-length cDNA project: theMammalian Gene Collection (MGC). Genome Res, 14: 2121-2127, 2004.
2. Tsukamoto H, Yoshitake H, Mori M, Yanagida M, Takamori K, Ogawa H, Takizawa T, Araki Y: Testicular proteins associated with the germ cell-marker, TEX101: involvement of cellubrevin in TEX101-trafficking to the cell surface during spermatogenesis. Biochem Biophys Res Commun, 345: 229-238, 2006.
3. Yoshitake H, Tsukamoto H, Maruyama-Fukushima M, Takamori K, Ogawa H, Araki Y: TEX101, a germ cell-marker glycoprotein, is associated with lymphocyte antigen 6 complex locus k within themouse testis. BiochemBiophys Res Commun, 372: 277-282, 2008.
4. Teng X, Yang J, Xie Y, Ni Z, Hu R, Shi L, Lin Z, Hu L, Zhao G, Ding X, Kong X: A novel spermatogenesis-specific uPAR gene expressed in human and mouse testis. Biochem Biophys Res Commun, 342: 1223-1227, 2006.
5. Jin H, Yoshitake H, Tsukamoto H, Takahashi M, Mori M, Takizawa T, Takamori K, Ogawa H, Kinoshita K, Araki Y: Molecular characterization of a germ-cell-specific antigen, TEX101, from mouse testis. Zygote, 14: 201-208, 2006. (Pubitemid 44032296)
6. Blasi F, Carmeliet P: uPAR: a versatile signalling orchestrator. Nat Rev Mol Cell Biol, 3: 932-943, 2002. (Pubitemid 35477371)
7. Memarzadeh S, Kozak KR, Chang L, Natarajan S, Shintaku P, Reddy ST, Farias-Eisner R: Urokinase plasminogen activator receptor: Prognostic biomarker for endometrial cancer. Proc Natl Acad Sci U S A, 99: 10647-10652, 2002.
8. Smith HW, Marshall CJ: Regulation of cell signalling by uPAR. Nat Rev Mol Cell Biol, 11: 23-36, 2010.
9. Lalou C, Scamuffa N, Mourah S, Plassa F, Podgorniak MP, Soufir N, Dumaz N, Calvo F, Basset-Seguin N, Khatib AM: Inhibition of the proprotein convertases represses the invasiveness of human primary melanoma cells with altered p53, CDKN2A and N-Ras genes. PLoS One, 5: e9992, 2010.
10. Sleight SB, Miranda PV, Plaskett NW, Maier B, Lysiak J, Scrable H, Herr JC, Visconti PE: Isolation and proteomic analysis of mouse sperm detergent-resistant membrane fractions: evidence for dissociation of lipid rafts during capacitation. Biol Reprod, 73: 721-729, 2005. (Pubitemid 41356131)
11. Tsukamoto H, Takizawa T, Takamori K, Ogawa H, Araki Y: Genomic organization and structure of the 5′-flanking region of the TEX101 gene: alternative promoter usage and splicing generate transcript variants with distinct 5′-untranslated region. Mol Reprod Dev, 74: 154-162, 2007. (Pubitemid 46047985)
12. Yin LL, Chung CM, Chen J, Fok KL, Ng CP, Jia RR, Ren X, Zhou J, Zhang T, Zhao XH, Lin M, Zhu H, Zhang XH, Tsang LL, Bi Y, Zhou Z, Mo F, Wong N, Chung YW, Sha J, Chan HC: A suppressor of multiple extracellular matrix-degrading proteases and cancer metastasis. J Cell Mol Med, 13: 4034-4041, 2009.
13. Ghafouri-Fard S, Abbasi A, Moslehi H, Faramarzi N, Taba Taba Vakili S, Mobasheri MB, Modarressi MH: Elevated expression levels of testis-specific genes TEX101 and SPATA19 in basal cell carcinoma and their correlation with clinical and pathological features. Br J Dermatol, 162: 772-729, 2010.
14. Lu H, Yu M, Sun Y, Mao W, Wang Q, Wu M, Han W: Expression and purification of bioactive high-purity mouse monokine induced by IFN-gamma in Escherichia coli. Protein Expr Purif, 55: 132-138, 2007. (Pubitemid 47259687)
15. Lee YM, Kang HJ, Jang M, Kim M, Bae KH, Chung SJ: Large-scale expression in Escherichia coli and efficient purification of precursor and active caspase-7 by introduction of thrombin cleavage sites. Protein Expr Purif, 69: 29-33, 2010.
16. Wu C, Wang Y, Zou M, Shan Y, Yao G, Wei P, Chen G, Wang J, Xu D: Prokaryotic expression, purification, and production of polyclonal antibody against human polypeptide N-acetylgalactosaminyltransferase 14. Protein Expr Purif, 56:1-7, 2007. (Pubitemid 47499083)
17. Ohlsson G, Moreira JM, Gromov P, Sauter G, Celis JE: Loss of expression of the adipocyte-type fatty acid-binding protein (A-FABP) is associatedwith progression of human urothelial carcinomas. Mol Cell Proteomics, 4: 570-581, 2005. (Pubitemid 40590573)
18. Abboud N, De Jesus M, Nakouzi A, Cordero RJ, Pujato M, Fiser A, Rivera J, Casadevall A: Identification of linear epitopes in Bacillus anthracis protective antigen bound by neutralizing antibodies. J Biol Chem, 284: 25077-25086, 2009.
19. Legleiter J, Lotz GP, Miller J, Ko J, Ng C, Williams GL, Finkbeiner S, Patterson PH, Muchowski PJ: Monoclonal antibodies recognize distinct conformational epitopes formed by polyglutamine in a mutant huntingtin fragment. J Biol Chem, 284: 21647-21658, 2009.
20. Feng XP, Yi H, Li MY, Li XH, Yi B, Zhang PF, Li C, Peng F, Tang CE, Li JL, Chen ZC, Xiao ZQ: Identification of biomarkers for predicting nasopharyngeal carcinoma response to radiotherapy by proteomics. Cancer Res, 70: 3450-3462, 2010.
21. Lu ZX, Ma XQ, Yang LF, Wang ZL, Zeng L, Li ZJ, Li XN, Tang M, Yi W, Gong JP, Sun LQ, Cao Y: DNAzymes targeted to EBV-encoded latent membrane protein-1 induce apoptosis and enhance radiosensitivity in nasopharyngeal carcinoma. Cancer Lett, 265: 226-238, 2008.
22. Liu T, Ding Y, Xie W, Li Z, Bai X, Li X, Fang W, Ren C, Wang S, Hoffman RM, Yao K: An imageable metastatic treatment model of nasopharyngeal carcinoma. Clin Cancer Res, 13: 3960-3967, 2007. (Pubitemid 47037604)
23. Chen Y, Ouyang GL, Yi H, Li MY, Zhang PF, Li C, Li JL, Liu YF, Chen ZC, Xiao ZQ: Identification of RKIP as an invasion suppressor protein in nasopharyngeal carcinoma by proteomic analysis. J Proteome Res, 7: 5254-5262, 2008.