Prokaryotic expression, purification, and production of polyclonal antibody against human polypeptide N-acetylgalactosaminyltransferase 14

Protein Expression and Purification

Volumn 56, Issue 1, 2007, Pages 1-7

  Wu, Chen ^a,b   Wang, YuanYuan ^a   Zou, MinJi ^a   Shan, YaoJun ^b   Yao, GuangYin ^b   Wei, Ping ^c   Chen, GuangYu ^a   Wang, JiaXi ^a   Xu, DongGang ^a  

^a INSTITUTE OF BASIC MEDICAL SCIENCES   (China)

^b HEBEI UNIVERSITY   (China)

^c SOUTHWEST HOSPITAL   (China)

Author keywords

GalNAc T14; Polyclonal antibody; Prokaryotic expression; Purification

Indexed keywords

HYBRID PROTEIN; N ACETYLGALACTOSAMINYLTRANSFERASE; POLYPEPTIDE N ACETYLGALACTOSAMINYLTRANSFERASE; POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE; UDP N ACETYL D GALACTOSAMINE POLYPEPTIDE N ACETYLGALACTOSAMINYLTRANSFERASE 14, HUMAN; UDP-N-ACETYL-D-GALACTOSAMINE POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE 14, HUMAN; UNCLASSIFIED DRUG;

ANIMAL; ARTICLE; ESCHERICHIA COLI; HUMAN; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; RABBIT; WESTERN BLOTTING;

ANIMALS; BLOTTING, WESTERN; CLONING, MOLECULAR; ESCHERICHIA COLI; HUMANS; N-ACETYLGALACTOSAMINYLTRANSFERASES; RABBITS; RECOMBINANT FUSION PROTEINS;

ESCHERICHIA COLI; MAMMALIA; ORYCTOLAGUS CUNICULUS; PROKARYOTA;

EID: 34848871893     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2007.04.027     Document Type: Article

References (20)

1. Dowbenko D., Andalibi A., Young P.E., Lusis A.J., and Lasky L.A. Structure and chromosomal localization of the murine gene encoding GLYCAM 1. A mucin-like endothelial ligand for l-selectin. J. Biol. Chem. 268 (1993) 4525-4529
2. Marinaro J.A., Neumann G.M., Russo V.C., Leeding K.S., and Bach L.A. O-Glycosylation of insulin-like growth factor (IGF) binding protein-6 maintains high IGF-II binding affinity by decreasing binding to glycosaminoglycans and susceptibility to proteolysis. Eur. J. Biochem. 267 (2000) 5378-5386
3. Kinloch R.A., Sakai Y., and Wassarman P.M. Mapping the mouse ZP3 combining site for sperm by exon swapping and site-directed mutagenesis. Proc. Natl. Acad. Sci. USA 92 (1995) 263-267
4. Sawada T., Ho J.J., Chung Y.S., Sowa M., and Kim Y.S. E-selectin binding by pancreatic tumor cells is inhibited by cancer sera. Int. J. Cancer 57 (1994) 901-907
5. Woodward H.D., Ringler N.J., Selvakumar R., Simet I.M., Bhavanandan V.P., and Davidson E.A. Deglycosylation studies on tracheal mucin glycoproteins. Biochemistry 26 (1987) 5315-5322
6. Brockhausen I. Pathways of O-glycan biosynthesis in cancer cells. Biochim. Biophys. Acta 1473 (1999) 67-95
7. McGuire E.J., and Roseman S. Enzymatic synthesis of the protein-hexosamine linkage in sheep submaxillary mucin. J. Biol. Chem. 242 (1967) 3745-3747
8. Ten Hagen K.G., Fritz T.A., and Tabak L.A. All in the family: the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases. Glycobiology 13 (2003) 1R-16R
9. Cheng L., Tachibana K., Iwasaki H., Kameyama A., Zhang Y., Kubota T., Hiruma T., Tachibana K., Kudo T., Guo J.M., and Narimatsu H. Characterization of a novel human UDP-GalNAc transferase, pp-GalNAc-T15. FEBS Lett. 566 (2004) 17-24
10. Wang H., Tachibana K., Zhang Y., Iwasaki H., Kameyama A., Cheng L., Guo J., Hiruma T., Togayachi A., Kudo T., Kikuchi N., and Narimatsu H. Cloning and characterization of a novel UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase, pp-GalNAc-T14. Biochem. Biophys. Res. Commun. 300 (2003) 738-744
11. Xu Z., and Weiss A. Negative regulation of CD45 by differential homodimerization of the alternatively spliced isoforms. Nat. Immunol. 3 (2002) 764-771
12. Moody A.M., Chui D., Reche P.A., Priatel J.J., Marth J.D., and Reinherz E.L. Developmentally regulated glycosylation of the CD8alphabeta coreceptor stalk modulates ligand binding. Cell 107 (2001) 501-512
13. Sauer J., Sigurskjold B.W., Christensen U., Frandsen T.P., Mirgorodskaya E., Harrison M., Roepstorff P., and Svensson B. Glucoamylase: structure/function relationships, and protein engineering. Biochim. Biophys. Acta 1543 (2000) 275-293
14. Garner B., Merry A.H., Royle L., Harvey D.J., Rudd P.M., and Thillet J. Structural elucidation of the N- and O-glycans of human apolipoprotein(a): role of o-glycans in conferring protease resistance. J. Biol. Chem. 276 (2001) 22200-22208
15. Freire T., Bay S., von Mensdorff-Pouilly S., and Osinaga E. Molecular basis of incomplete O-glycan synthesis in MCF-7 breast cancer cells: putative role of MUC6 in Tn antigen expression. Cancer Res. 65 (2005) 7880-7887
16. Qiu H., Guo X.H., Mo J.H., Jin M.F., Wu S.L., and Chen H.L. Expressions of polypeptide: N-acetylgalactosaminyltransferase in leukemia cell lines during 1,25-dihydroxyvitamin D3 induced differentiation. Glycoconj. J. 23 (2006) 575-584
17. Young Jr. W.W., Holcomb D.R., Ten Hagen K.G., and Tabak L.A. Expression of UDP-GalNAc: polypeptide N-acetylgalactosaminyltransferase isoforms in murine tissues determined by real-time PCR: a new view of a large family. Glycobiology 13 (2003) 549-557
18. Shibao K., Izumi H., Nakayama Y., Ohta R., Nagata N., Nomoto M., Matsuo K., Yamada Y., Kitazato K., Itoh H., and Kohno K. Expression of UDP-N-acetyl-alpha-d-galactosamine-polypeptide galNAc N-acetylgalactosaminyltransferase-3 in relation to differentiation and prognosis in patients with colorectal carcinoma. Cancer 94 (2002) 1939-1946
19. Hagen F.K., Hazes B., Raffo R., deSa D., and Tabak L.A. Structure-function analysis of the UDP-N-acetyl-d-galactosamine:polypeptide N-acetylgalactosaminyltransferase. Essential residues lie in a predicted active site cleft resembling a lactose repressor fold. J. Biol. Chem. 274 (1999) 6797-6803
20. Wu C., Yao G.Y., Zou M.J., Chen G.Y., Wang M., Liu J.Q., Wang J.X., and Xu D.G. N-acetylgalactosaminyltransferase 14, a novel insulin-like growth factor binding protein-3 binding partner. Biochem. Biophys. Res. Commun. 357 (2007) 360-365