Isolation of brain mitochondria from neonatal mice

Journal of Neurochemistry

Volumn 119, Issue 6, 2011, Pages 1253-1261

  Wang, Xiaoyang ^a,b   Leverin, Anna Lena ^a   Han, Wei ^a,b   Zhu, Changlian ^a,b   Johansson, Bengt R ^a   Jacotot, Etienne ^c,d,e   Ten, Vadim S ^f   Sims, Neil R ^g   Hagberg, Henrik ^a,c,h  

^a UNIVERSITY OF GOTHENBURG   (Sweden)

^b ZHENGZHOU UNIVERSITY   (China)

^c IMPERIAL COLLEGE LONDON   (United Kingdom)

^d HÔPITAL ROBERT DEBRÉ   (France)

^e UNIVERSITÉ PARIS DESCARTES   (France)

^f Och Spine at New York Presbyterian Hospitals   (United States)

^g FLINDERS UNIVERSITY   (Australia)

^h UNIVERSITY OF GOTHENBURG   (Sweden)

Author keywords

brain; mitochondrial isolation; mouse; neonatal

Indexed keywords

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BRAIN MITOCHONDRION; CELL FRACTIONATION; CONTROLLED STUDY; CYTOSOL; DENSITY GRADIENT CENTRIFUGATION; FEMALE; INTERMETHOD COMPARISON; ISOLATION PROCEDURE; MITOCHONDRIAL ISOLATION; MITOCHONDRIAL RESPIRATION; MOUSE; NEWBORN; NONHUMAN; PRIORITY JOURNAL; SYNAPTOSOME;

ADENOSINE DIPHOSPHATE; ANIMALS; ANIMALS, NEWBORN; BRAIN; ELECTRON TRANSPORT COMPLEX IV; L-LACTATE DEHYDROGENASE; MICE; MICE, INBRED C57BL; MICROSCOPY, ELECTRON; MITOCHONDRIA; SUBCELLULAR FRACTIONS; SYNAPTOSOMES;

MUS; RATTUS;

EID: 82955247596     PISSN: 00223042     EISSN: 14714159     Source Type: Journal    
DOI: 10.1111/j.1471-4159.2011.07525.x     Document Type: Article

References (30)

1. Blomgren K., Zhu C., Hallin U., and, Hagberg H., (2003) Mitochondria and ischemic reperfusion damage in the adult and in the developing brain. Biochem. Biophys. Res. Commun. 304, 551-559. (Pubitemid 36506528)
2. Caspersen C. S., Sosunov A., Utkina-Sosunova I., Ratner V. I., Starkov A. A., and, Ten V. S., (2008) An isolation method for assessment of brain mitochondria function in neonatal mice with hypoxic-ischemic brain injury. Dev. Neurosci. 30, 319-324.
3. Felderhoff-Mueser U., Taylor D. L., Greenwood K., Kozma M., Stibenz D., Joashi U. C., Edwards A. D., and, Mehmet H., (2000) Fas/CD95/APO-1 can function as a death receptor for neuronal cells in vitro and in vivo and is upregulated following cerebral hypoxic-ischemic injury to the developing rat brain. Brain Pathol. 10, 17-29. (Pubitemid 30056390)
4. Frezza C., Cipolat S., and, Scorrano L., (2007) Organelle isolation: functional mitochondria from mouse liver, muscle and cultured fibroblasts. Nat. Protoc. 2, 287-295. (Pubitemid 47040043)
5. Galluzzi L., Blomgren K., and, Kroemer G., (2009) Mitochondrial membrane permeabilization in neuronal injury. Nat. Rev. Neurosci. 10, 481-494.
6. Gilland E., and, Hagberg H., (1996) NMDA Receptor-dependent increase of cerebral glucose utilization after hypoxia-ischemia in the immature rat. J. Cereb. Blood Flow Metab. 16, 1005-1013. (Pubitemid 26292069)
7. Gilland E., Bona E., and, Hagberg H., (1998a) Temporal changes of regional glucose use, blood flow, and microtubule-associated protein 2 immunostaining after hypoxia-ischemia in the immature rat brain. J. Cereb. Blood Flow Metab. 18, 222-228. (Pubitemid 28082520)
8. Gilland E., Puka-Sundvall M., Hillered L., and, Hagberg H., (1998b) Mitochondrial function and energy metabolism after hypoxia-ischemia in the immature rat brain: involvement of NMDA-receptors. J. Cereb. Blood Flow Metab. 18, 297-304. (Pubitemid 28103637)
9. Hagberg H., Mallard C., Rousset C. I., and, Xiaoyang W., (2009) Apoptotic mechanisms in the immature brain: involvement of mitochondria. J. Child Neurol. 24, 1141-1146.
10. Hansson M. J., Persson T., Friberg H., Keep M. F., Rees A., Wieloch T., and, Elmer E., (2003) Powerful cyclosporin inhibition of calcium-induced permeability transition in brain mitochondria. Brain Res. 960, 99-111. (Pubitemid 36044567)
11. Hansson M. J., Mansson R., Mattiasson G., Ohlsson J., Karlsson J., Keep M. F., and, Elmer E., (2004) Brain-derived respiring mitochondria exhibit homogeneous, complete and cyclosporin-sensitive permeability transition. J. Neurochem. 89, 715-729. (Pubitemid 38534157)
12. Hansson M. J., Mansson R., Morota S., et al. (2008) Calcium-induced generation of reactive oxygen species in brain mitochondria is mediated by permeability transition. Free Radic. Biol. Med. 45, 284-294.
13. Kroemer G., Galluzzi L., and, Brenner C., (2007) Mitochondrial membrane permeabilization in cell death. Physiol. Rev. 87, 99-163. (Pubitemid 46209992)
14. Kuznetsov A. V., Veksler V., Gellerich F. N., Saks V., Margreiter R., and, Kunz W. S., (2008) Analysis of mitochondrial function in situ in permeabilized muscle fibers, tissues and cells. Nat. Protoc. 3, 965-976. (Pubitemid 351818685)
15. Musatov A., Ortega-Lopez J., and, Robinson N. C., (2000) Detergent-solubilized bovine cytochrome c oxidase: dimerization depends on the amphiphilic environment. Biochemistry 39, 12996-13004.
16. Ness J. M., Harvey C. A., Strasser A., Bouillet P., Klocke B. J., and, Roth K. A., (2006) Selective involvement of BH3-only Bcl-2 family members Bim and Bad in neonatal hypoxia-ischemia. Brain Res. 1099, 150-159. (Pubitemid 44088651)
17. Northington F. J., Ferriero D. M., Flock D. L., and, Martin L. J., (2001) Delayed neurodegeneration in neonatal rat thalamus after hypoxia-ischemia is apoptosis. J. Neurosci. 21, 1931-1938. (Pubitemid 32221377)
18. Puka-Sundvall M., Wallin C., Gilland E., Hallin U., Wang X., Sandberg M., Karlsson J., Blomgren K., and, Hagberg H., (2000) Impairment of mitochondrial respiration after cerebral hypoxia-ischemia in immature rats: relationship to activation of caspase-3 and neuronal injury. Brain Res. Dev. Brain Res. 125, 43-50. (Pubitemid 32057180)
19. Rajapakse N., Shimizu K., Payne M., and, Busija D., (2001) Isolation and characterization of intact mitochondria from neonatal rat brain. Brain Res. Brain Res. Protoc. 8, 176-183. (Pubitemid 33152942)
20. Schapira A. H., (2008) Mitochondrial dysfunction in neurodegenerative diseases. Neurochem. Res. 33, 2502-2509.
21. Sims N. R., (1990) Rapid isolation of metabolically active mitochondria from rat brain and subregions using Percoll density gradient centrifugation. J. Neurochem. 55, 698-707. (Pubitemid 120021648)
22. Sims N. R., and, Anderson M. F., (2008) Isolation of mitochondria from rat brain using Percoll density gradient centrifugation. Nat. Protoc. 3, 1228-1239.
23. Tasdemir E., Maiuri M. C., Galluzzi L., et al. (2008) Regulation of autophagy by cytoplasmic p53. Nat. Cell Biol. 10, 676-687. (Pubitemid 351772933)
24. Toman J., and, Fiskum G., (2011) Influence of aging on membrane permeability transition in brain mitochondria. J. Bioenerg. Biomembr. 43, 3-10.
25. Trichet A., Bezault M., Ankri S., Jacotot E., and, Chauvier D., (2008) Evidence for membrane permeabilization of neonatal cortical brain mitochondria during perinatal arterial stroke in rats: ex vivo and in organello observations. Acta Paediatr. 97, 17-17.
26. Wang X., Karlsson J. O., Zhu C., Bahr B. A., Hagberg H., and, Blomgren K., (2001) Caspase-3 activation after neonatal rat cerebral hypoxia-ischemia. Biol. Neonate 79, 172-179. (Pubitemid 32247082)
27. Wang X., Zhu C., Qiu L., Hagberg H., Sandberg M., and, Blomgren K., (2003) Activation of ERK1/2 after neonatal rat cerebral hypoxia-ischaemia. J. Neurochem. 86, 351-362. (Pubitemid 36842434)
28. Wang X., Carlsson Y., Basso E., et al. (2009) Developmental shift of cyclophilin D contribution to hypoxic-ischemic brain injury. J. Neurosci. 29, 2588-2596.
29. Zhivotovsky B., Galluzzi L., Kepp O., and, Kroemer G., (2009) Adenine nucleotide translocase: a component of the phylogenetically conserved cell death machinery. Cell Death Differ. 16, 1419-1425.
30. Zhu C., Wang X., Huang Z., et al. (2007) Apoptosis-inducing factor is a major contributor to neuronal loss induced by neonatal cerebral hypoxia-ischemia. Cell Death Differ. 14, 775-784. (Pubitemid 46444515)