Analysis of the surface proteins of Acidithiobacillus ferrooxidans strain SP5/1 and the new, pyrite-oxidizing Acidithiobacillus isolate HV2/2, and their possible involvement in pyrite oxidation

Archives of Microbiology

Volumn 193, Issue 12, 2011, Pages 867-882

  Klingl, Andreas ^a   Moissl Eichinger, Christine ^a   Wanner, Gerhard ^b   Zweck, Josef ^a   Huber, Harald ^a   Thomm, Michael ^a   Rachel, Reinhard ^a  

^a UNIVERSITY OF REGENSBURG   (Germany)

^b UNIVERSITY OF MUNICH   (Germany)

Author keywords

Acidithiobacillus; Cell surface; Electron microscopy; EPS; High pressure freezing; Pyrite; S layer; Thiobacillus

Indexed keywords

MEMBRANE PROTEIN; PYRITE;

ACIDITHIOBACILLUS FERROOXIDANS; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM ISOLATE; CELL ADHESION; CELL SHAPE; CELL SURFACE; CONTROLLED STUDY; FREEZING; GENE IDENTIFICATION; GENE SEQUENCE; MICROBIAL MORPHOLOGY; MOLECULAR PHYLOGENY; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROKARYOTES BY METABOLISM; PROTEIN DETERMINATION; PROTEIN FUNCTION; PYRITE OXIDIZING BACTERIUM; RNA 16S GENE; SCANNING ELECTRON MICROSCOPY; TRANSMISSION ELECTRON MICROSCOPY;

ACIDITHIOBACILLUS; BACTERIAL PROTEINS; CELL MEMBRANE; FIMBRIAE, BACTERIAL; FLAGELLA; IRON; MEMBRANE GLYCOPROTEINS; MICROSCOPY, ELECTRON, SCANNING; MICROSCOPY, ELECTRON, TRANSMISSION; MOLECULAR SEQUENCE DATA; OXIDATION-REDUCTION; PERIPLASM; PHYLOGENY; RNA, RIBOSOMAL, 16S; SULFIDES;

ACIDITHIOBACILLUS; ACIDITHIOBACILLUS FERROOXIDANS; BACTERIA (MICROORGANISMS); NEGIBACTERIA; THIOBACILLUS;

EID: 82355169822     PISSN: 03028933     EISSN: 1432072X     Source Type: Journal    
DOI: 10.1007/s00203-011-0720-y     Document Type: Article

References (81)

1. W Baumeister G Lembcke 1992 Structural features of archaebacterial cell envelopes J Bioenerg Biomembr 24 567 575 1459988 10.1007/BF00762349 1:CAS:528:DyaK3sXjsFGhug%3D%3D
2. DP Bayley SF Koval 1994 Membrane association and isolation of the S-layer protein of Methanoculleus marisnigri Can J Microbiol 40 237 241 10.1139/m94-039 (Pubitemid 24174894)
3. TJ Beveridge PH Pouwels M Sára A Kotiranta K Lounatmaa K Kari E Kerosuo M Haapasalo EM Egelseer I Schocher UB Sleytr L Morelli ML Callegari JF Nomellini WH Bingle J Smit E Leibovitz M Lemaire I Miras S Salamitou P Béguin H Ohayon P Gounon M Matuschek SF Koval 1997 Functions of S-layers FEMS Microbiol Rev 20 99 149 9276929 10.1016/S0168-6445(97)00043-0 1:CAS:528:DyaK2sXltVCksbw%3D (Pubitemid 27373489)
4. T Burghardt M Saller S Gürster D Müller C Meyer U Jahn E Hochmuth R Deutzmann F Siedler P Babinger R Wirth H Huber R Rachel 2008 Insight into the proteome of the hyperthermophilic Crenarchaeon Ignicoccus hospitalis: the major cytosolic and membrane proteins Arch Microbiol 190 379 394 18584152 10.1007/s00203-008-0399-x 1:CAS:528:DC%2BD1cXhtFOmtrfI
5. P Carter 1971 Spectrophotometric determination of serum iron at the submicrogram level with a new reagent (ferrozine) Anal Biochem 40 450 458 5551554 10.1016/0003-2697(71)90405-2 1:CAS:528:DyaE3MXhtlKiurg%3D
6. G-W Cheong Z Cejka J Peters KO Stetter W Baumeister 1991 The surface protein layer of Methanoplanus limicola: three-dimensional structure and chemical characterization System Appl Microbiol 14 209 217 1:CAS:528: DyaK38Xjt1Gq
7. 2+ oxidation by Acidithiobacillus ferrooxidans Miner Eng 19 960 967 10.1016/j.mineng.2005.10.024 1:CAS:528:DC%2BD28XkslGktrs%3D (Pubitemid 43674507)
8. J Eichler 2003 Facing extremes: archaeal surface-layer (glyco)proteins Microbiology 149 3347 3351 14663068 10.1099/mic.0.26591-0 1:CAS:528: DC%2BD3sXhtVWnsL7K (Pubitemid 38016663)
9. H Engelhardt J Peters 1998 Structural research on surface layers: a focus on stability, surface layer homology domains, and surface layer-cell wall interactions J Struct Biol 124 276 302 10049812 10.1006/jsbi.1998.4070 1:CAS:528:DyaK1MXhtlKlsL4%3D (Pubitemid 29143066)
10. K Etzel A Klingl H Huber R Rachel G Schmalz M Thomm W Depmeier 2008 Etching of 111 and 210 synthetic pyrite surfaces by two archaeal strains, Metallosphaera sedula and Sulfolobus metallicus Hydrometallurgy 94 116 120 10.1016/j.hydromet.2008.05.026 1:CAS:528:DC%2BD1cXht1aru7rM
11. CM Fletcher MJ Coyne DL Bentley OF Villa LE und Comstock 2007 Phase-variable expression of a family of glycoproteins imparts a dynamic surface to a symbiont in its human intestinal ecosystem PNAS 104 2413 2418 17284602 10.1073/pnas.0608797104 1:CAS:528:DC%2BD2sXisVShtrw%3D (Pubitemid 46391411)
12. TA Fowler PR Holmes FK Crundwell 1999 Mechanism of pyrite dissolution in the presence of Thiobacillus ferrooxidans Appl Environ Microbiol 65 2987 2993 10388693 1:CAS:528:DyaK1MXktlemtL0%3D (Pubitemid 29315570)
13. T Gehrke J Telegdi D Thierry W Sand 1998 Importance of extracellular polymeric substances from Thiobacillus ferrooxidans for bioleaching Appl Environ Microbiol 64 2743 2747 9647862 1:CAS:528:DyaK1cXkt1Ogsb8%3D (Pubitemid 28303332)
14. YA Gorby S Yanina JS McLean KM Rosso D Moyles A Dohnalkova TJ Beveridge IS Chang BH Kim KS Kim DE Culley SB Reed MF Romine DA Saffarini EA Hill L Shi DA Elias DW Kennedy G Pinchuk K Watanabe S Ishii B Logan KH Nealson JK Fredrickson 2006 Electrically conductive bacterial nanowires produced by Shewanella oneidensis strain MR-1 and other microorganisms Proc Natl Acad Sci 103 11358 11363 16849424 10.1073/pnas.0604517103 1:CAS:528:DC%2BD28XnvVarsrs%3D (Pubitemid 44156522)
15. DW Grogan 1996 Organization and interactions of cell envelope proteins of the extreme thermoacidophile Sulfolobus acidocaldarius Can J Microbiol 42 1163 1171 10.1139/m96-148 1:CAS:528:DyaK28Xnt1yjs7Y%3D (Pubitemid 26419617)
16. Gruber K, Sleytr UB (1988) Localized insertion of new S-layer during growth of Bacillus stearothermophilus strain PV72. EMBO Workshop on crystalline bacterial surface layers. Springer, Heidelberg
17. KB Hallberg E González-Toril DB und Johnson 2010 Acidithiobacillus ferrivorans, sp. nov.; facultatively anaerobic, psychrotolerant iron- and sulfur-oxidizing acidophiles isolated from metal mine-impacted environments Extremophiles 14 9 19 19787416 10.1007/s00792-009-0282-y 1:CAS:528: DC%2BC3cXjtlCitA%3D%3D
18. K Harneit A Göksel D Kock JH Klock T Gehrke W Sand 2006 Adhesion to metal sulfide surfaces by cells of Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans and Leptospirillum ferrooxidans Hydrometallurgy 83 245 254 10.1016/j.hydromet.2006.03.044 1:CAS:528:DC%2BD28XnsFGhtbw%3D (Pubitemid 44118574)
19. Hofte M (1993) Classes of microbial siderophores. In: Barton LL, Hemming BC (eds) Iron Chelation in Plants and Soil Microorganisms. Academic Press, San Diego, p 4
20. H Hohenberg K Mannweiler M Müller 1994 High-pressure freezing of cell suspensions in cellulose capillary tubes J Microsc 175 34 43 7932676 10.1111/j.1365-2818.1994.tb04785.x 1:STN:280:DyaK2M%2FhvVWrtg%3D%3D (Pubitemid 24246592)
21. Huber H (1987) Isolierung, Charakterisierung und taxonomische Einordnung neuer mesophiler, metallmetabolisierender Bakterien. PhD thesis. University of Regensburg, Germany
22. G Huber H Huber KO Stetter 1986 Isolation and characterization of new metal-mobilizing bacteria Biotechnol Bioeng Symp 16 239 251 1:CAS:528: DyaL28Xktl2jsrw%3D (Pubitemid 17607598)
23. C Jiang Y Liu Y Liu X Guo SJ Liu 2009 Isolation and characterization of ferrous- and sulfur-oxidizing bacteria from Tengchong solfataric region, China J Environ Sci (China) 21 1247 1252 10.1016/S1001-0742(08)62411-0 1:CAS:528:DC%2BD1MXht1ahtLvK
24. RA Jones SF Koval HW Nesbitt 2003 Surface alteration of arsenopyrite (FeAsS) by Thiobacillus ferrooxidans Geochim Cosmochim Acta 67 955 965 10.1016/S0016-7037(02)00996-1 1:CAS:528:DC%2BD3sXht1WgtLg%3D (Pubitemid 36257800)
25. B Junglas A Briegel T Burghardt P Walther R Wirth H Huber R Rachel 2008 Ignicoccus hospitalis and Nanoarchaeum equitans: ultrastructure, cell-cell interaction, and 3D reconstruction from serial sections of freeze-substituted cell and by electron cryotomography Arch Microbiol 190 395 408 18622597 10.1007/s00203-008-0402-6 1:CAS:528:DC%2BD1cXhtFOmtrfF
26. U Kärcher H Schröder E Haslinger G Allmaier R Schreiner F Wieland A Haselbeck H und König 1993 Primary structure of the heterosaccharide of the surface glycoprotein of Methanothermus fervidus J Biol Chem 268 26821 26826 8262914 (Pubitemid 24006386)
27. DW Kelly AP Wood 2000 Reclassification of some species of Thiobacillus to the newly designated genera Acidithiobacillus gen. nov., Halothiobacillus gen. nov. and Thermithiobacillus gen. nov IJSEM 50 511 516 10758854 (Pubitemid 30167492)
28. König H, Rachel R, Claus H (2007) Proteinaceous surface layers of Archaea: ultrastructure and biochemistry. In: Cavicchioli R (ed) Archaea: molecular and cell biology. American Society of Microbiology Press, Washington, DC, USA, pp 315-340
29. L Larsson G Olsson O Holst HT Karlsson 1993 Oxidation of pyrite by Acidianus brierleyi: Importance of close contact between the pyrite and the microorganisms Biotechnol Lett 15 99 104 10.1007/BF00131561 1:CAS:528: DyaK3sXhs1Cqt7c%3D (Pubitemid 23075006)
30. Lazar P (2004) Optimierung der Gewinnung von extrazellulären polymeren Substanzen von Laugungsbakterien. Diploma thesis. University of Hamburg, Germany
31. S-W Lee M Sabet H-S Um J Yang HC Kim W Zhu 2006 Identification and characterization of the genes encoding a unique surface (S-) layer of Tannerela forsythia Gene 371 102 111 16488557 10.1016/j.gene.2005.11.027 1:CAS:528:DC%2BD28Xisl2rsbg%3D
32. H-L Liu B-Y Chen Y-W Lan Y-C Cheng 2003 SEM and AFM images of pyrite surfaces avter bioleaching by the indigenous Thiobacillus thiooxidans Appl Microbiol Biotechnol 62 414 420 12719934 10.1007/s00253-003-1280-0 1:CAS:528:DC%2BD3sXmslClsr4%3D (Pubitemid 37214926)
33. W Ludwig O Strunk N Klugbauer M Weizenegger J Neumaier M Bachleitner KH Schleifer 1998 Bacterial phylogeny based on comparative sequence analysis Electrophoresis 19 554 568 9588802 10.1002/elps.1150190416 1:CAS:528: DyaK1cXisVentrY%3D (Pubitemid 28179960)
34. W Ludwig O Strunk R Westram L Richter H Meier Yedhukumar A Buchner T Lai S Steppi G Jobb W Förster I Brettske S Gerber AW Ginhart O Gross S Grumann S Hermann R Jost A König T Liss R Lümann M May B Nonhoff B Reichel R Strehlow A Stamatakis N Stuckmann A Vilbig M Lenke T Ludwig A Bode K-H Schleifer 2004 ARB: a software environment for sequence data Nucl Acids Res 32 1363 1371 14985472 10.1093/nar/gkh293 1:CAS:528:DC%2BD2cXhvFSqu7k%3D (Pubitemid 38854737)
35. ML Merroun J Raff A Rossberg C Hennig T Reich S Selenska-Pobell 2005 Complexation of uranium by cells and S-layer sheets of Bacillus sphaericus JG-A12 Appl Environ Microbiol 71 5532 5543 16151146 10.1128/AEM.71.9.5532-5543. 2005 1:CAS:528:DC%2BD2MXhtVahtr3E (Pubitemid 41341376)
36. MF Mescher JL Strominger 1976 Structural (shape-maintaining) role of the cell surface glycoprotein of Halobacterium salinarium PNAS 73 2687 2691 1066681 10.1073/pnas.73.8.2687 1:CAS:528:DyaE28XltF2gtrY%3D
37. S Mesnage T Fontaine T Mignot M Delepierre M Mock A und Fouet 2000 Bacterial SLH domain proteins are non-covalently anchored to the cell surface via a conserved mechanism involving wall polysaccharide pyruvylation EMBO J 19 4473 4484 10970841 10.1093/emboj/19.17.4473 1:CAS:528:DC%2BD3cXmslGkur4%3D
38. P Messner 2004 Prokaryotic glycoproteins: Unexplored but Important J Bacteriol 186 2517 2519 15090489 10.1128/JB.186.9.2517-2519.2004 1:CAS:528:DC%2BD2cXjsFKrsbY%3D (Pubitemid 38525902)
39. Messner P, Schäffer C (2000) Surface layer glycoproteins of Bacteria and Archaea. In: Doyle RJ (ed) Glycomicrobiology. Kluwer Academic Publisher, New York, pp 93-125
40. P Messner C Schäffer 2003 Prokaryotic glycoproteins Prog Chem Org Nat Prod 85 51 124 1:CAS:528:DC%2BD3sXhs1Wht7w%3D
41. P Messner D Pum M Sára KO Stetter UB Sleytr 1986 Ultrastructure of the cell envelope of the archaebacteria Thermoproteus tenax and Thermoproteus neutrophilus J Bacteriol 166 1046 1054 3086286 1:CAS:528:DyaL28Xktlyru7Y%3D (Pubitemid 16098192)
42. Mjoli N, Kulpa CF (1988) Identification of a unique outer membrane protein required for iron oxidation in Thiobacillus ferrooxidans. In: Norris PR, Kelly DP (eds) Biohydrometallurgy-Proceedings of the International Symposium Warwick 1987: pp 89-103. Science and Technology Letters. Antony Rowe Ltd., Kew, Great Britain
43. J Peters M Nitsch B Kühlmorgen R Golbik A Lupas J Kellermann H Engelhardt J-P Pfander S Müller K Goldie A Engel K-O Stetter W Baumeister 1995 Tetrabrachion: a filamentous archaebacterial surface protein assembly of unusual structure and extreme stability J Mol Biol 245 385 401 7837271 10.1006/jmbi.1994.0032 1:CAS:528:DyaK2MXjtlClur8%3D
44. U Pley J Schipka A Gambacorta HW Jannasch H Fricke R Rachel KO Stetter 1991 Pyrodictium abyssi sp. nov. represents a novel heterotrophic marine archaeal hyperthermophile growing at 110°C System Appl Microbiol 14 245 253
45. D Pum P Messner UB Sleytr 1991 Role of the S-layer in morphogenesis and cell division of the archaebacterium Methanocorpusculum sinense J Bacteriol 173 6865 6873 1938891 1:CAS:528:DyaK3MXmslGmtrs%3D
46. Rachel R (1999) Fine structure of hyperthermophilic prokaryotes. In: Seckbach J (ed) Enigmatic microorganisms and life in extreme environments. Kluwer Academic Publisher, The Netherlands, pp 277-289
47. R Rachel AM Engel R Huber KO Stetter W Baumeister 1990 A porin-type protein is the main constituent of the cell envelope of the ancestral eubacterium Thermotoga maritima FEBS Lett 262 64 68 10.1016/0014-5793(90)80155-C 1:CAS:528:DyaK3cXhvFGjsrY%3D (Pubitemid 20104262)
48. R Rachel D Pum J Šmarda D Šmajs J Komrska V Krzyzánek G Rieger KO Stetter 1997 II Fine structure of S-layers FEMS Microbiol Rev 20 13 23 10.1016/S0168-6445(97)00040-5 1:CAS:528: DyaK2sXltVCksLc%3D (Pubitemid 27373486)
49. R Rachel I Wyschkony S Riehl H Huber 2002 The ultrastructure of Ignicoccus: evidence for a novel outer membrane and for intracellular vesicle budding in an archaeon Archaea 1 9 18 15803654 10.1155/2002/307480 1:CAS:528:DC%2BD38XitFCmsrg%3D (Pubitemid 38871626)
50. R Rachel C Meyer A Klingl S Gürster T Heimerl N Wasserburger T Burghardt U Küper A Bellack S Schopf R Wirth H Huber G Wanner 2010 Analysis of the ultrastructure of archaea by electron microscopy Method Cell Biol 96 47 69 10.1016/S0091-679X(10)96003-2
51. G Reguera KD McCarthy T Mehta JS Nicoll MT Tuominen DR Lovley 2005 Extracellular electron transfer via microbial nanowires Nature 435 1098 1101 15973408 10.1038/nature03661 1:CAS:528:DC%2BD2MXltl2ntrc%3D (Pubitemid 40910516)
52. ES Reynolds 1963 Use of lead citrate as a stain in electron microscopy Cell Biol 17 208 213 10.1083/jcb.17.1.208 1:CAS:528:DyaF3sXktVClu70%3D
53. Ristl R, Steiner K, Zarschler K, Zayni S, Messner P, Schäffer C (2011) The S-layer glycome-adding to the sugar coat of bacteria. Int J Microbiol 2011
54. T Rohwerder T Gehrke K Kinzler W Sand 2003 Bioleaching review part A: progress in bioleaching: fundamentals and mechanisms of bacterial metal sulfide oxidation Appl Microbiol Biotechnol 63 239 248 14566432 10.1007/s00253-003-1448- 7 1:CAS:528:DC%2BD3sXpsFOnt7o%3D (Pubitemid 38130572)
55. AL Rose TD Waite 2003 Kinetics of iron complexation by dissolved natural organic matter in coastal waters Mar Chem 84 85 103 10.1016/S0304-4203(03)00113- 0 1:CAS:528:DC%2BD3sXoslOrsrw%3D (Pubitemid 37545904)
56. W Sand T Gehrke 2006 Extracellular polymeric substances mediate bioleaching/biocorrosion via interfacial processes involving iron(III) ions and acidophilic bacteria Res Microbiol 157 49 56 16431087 10.1016/j.resmic.2005.07. 012 1:CAS:528:DC%2BD28Xhtlaku74%3D (Pubitemid 43202117)
57. LE Sandercock AM MacLeod E Ong RAJ Warren 1994 Non-S-layer glycoproteins in eubacteria FEMS Microbiol Lett 118 1 8 8013865 10.1111/j.1574-6968.1994. tb06795.x 1:CAS:528:DyaK2cXktVCmtLY%3D (Pubitemid 24165131)
58. M Sára UB Sleytr 2000 S-Layer Proteins J Bacteriol 182 859 868 10648507 10.1128/JB.182.4.859-868.2000 (Pubitemid 30075004)
59. WO Saxton 1996 Semper: distortion compensation, selective averaging, 3-D reconstruction and transfer function correction in a highly programmable system J Struct Biol 116 230 236 8812977 10.1006/jsbi.1996.0035 1:CAS:528: DyaK28XitVKiurY%3D (Pubitemid 26093148)
60. C Schäffer P Messner 2001 Glycobiology of surface layer proteins Biochimie 83 591 599 11522387 10.1016/S0300-9084(01)01299-8 (Pubitemid 32827342)
61. C Schäffer T Wugeditsch H Kählig A Scheberl S Zayni P Messner 2002 The surface layer (s-layer) glycoprotein of Geobacillus stearothermophilus NRS 2004/3a J Biol Chem 277 6230 6239 11741945 10.1074/jbc.M108873200 (Pubitemid 34968414)
62. H Schägger G von Jagow 1987 Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa Anal Biochem 166 223 231 10.1016/0003-2697(87)90587-2 (Pubitemid 18004907)
63. T Schirmer 1998 General and specific porins from bacterial outer membranes J Struct Biol 121 101 109 9615433 10.1006/jsbi.1997.3946 1:CAS:528:DyaK1cXjslGht70%3D (Pubitemid 28361439)
64. Schuster KU (2003) Der Surface Layer von "Nanoarchaeum equitans". Diploma thesis. University of Regensburg, Germany
65. JP Segrest RL Jackson 1972 Molecular weight determination of glycoproteins by polyacrylamide gel electrophoresis in sodium dodecyl sulphate Method Enzymol 28 54 63 10.1016/0076-6879(72)28007-7
66. GE Shears DA Ledward RJ Neale 1987 Iron complexation to carboxyl groups in a bovine serum albumin digest Int J Food Sci Tech 22 265 272 10.1111/j.1365-2621.1987.tb00487.x 1:CAS:528:DyaL2sXlslCmtrg%3D
67. MP Silverman DG Lundgren 1959 Studies on the chemoautotrophic iron bacterium Ferrobacillus ferrooxidans J Bacteriol 78 326 331 14446765 1:CAS:528:DyaF3cXltV2quw%3D%3D
68. UB Sleytr 1997 I Basic and applied S-layer research FEMS Microbiol Rev 20 5 12 10.1016/S0168-6445(97)00039-9 1:CAS:528:DyaK2sXltVCksLY%3D (Pubitemid 27373485)
69. Sleytr UB, Messner P, Pum D, Sára M (1988) Crystalline bacterial cell surface layers. EMBO Workshop on crystalline bacterial surface layers. Springer, Heidelberg
70. M Stieglmeier R Wirth G Kminek C Moissl-Eichinger 2009 Cultivation of Anaerobic and Facultatively Anaerobic Bacteria from Spacecraft-associated Clean Rooms Appl Environ Micrbiol 75 3484 3491 10.1128/AEM.02565-08 1:CAS:528:DC%2BD1MXntlShurY%3D
71. D Studer W Graber A Al-Moudi P Eggli 2001 A new approach for cryofixation by high-pressure freezing J Microsc 203 285 294 11555146 10.1046/j.1365-2818. 2001.00919.x 1:CAS:528:DC%2BD3MXntFGgt7w%3D (Pubitemid 33755967)
72. J Valdés I Pedroso R Quatrini RJ Dodson H Tettelin R Blake II JA Eisen DS Holmes 2008 Acidithiobacillus ferrooxidans metabolism: from genome sequence to industrial applications BMC Genomics 9 1 24 10.1186/1471-2164-9-597
73. A Veith A Klingl B Zolghadr K Lauber R Mentele F Lottspeich R Rachel S-V Albers A Kletzin 2009 Acidianus, Sulfolobus and Metallosphaera surface layers: structure, composition and gene expression Mol Microbiol 73 58 72 19522740 10.1111/j.1365-2958.2009.06746.x 1:CAS:528:DC%2BD1MXovVGqurc%3D
74. P Walther A Ziegler 2002 Freeze substitution of high-pressure frozen samples: the visibility of biological membranes is improved when the substitution medium contains water J Microsc 208 3 10 12366592 10.1046/j.1365-2818.2002.01064.x 1:CAS:528:DC%2BD38XotlOjtLo%3D (Pubitemid 35365577)
75. F Wieland J Lechner M Sumper 1982 The cell wall glycoprotein of Halobacteria: structural, functional and biosynthetic aspects Zbl Bakt Hyg I Abt Orig C 3 161 170 1:CAS:528:DyaL38XktlGksLg%3D (Pubitemid 12103064)
76. F Wieland R Heitzer W Schaefer 1983 Asparaginylglucose: novel type of carbohydrate linkage PNAS 80 5470 5474 16593364 10.1073/pnas.80.18.5470 1:CAS:528:DyaL3sXlsFCjtbo%3D (Pubitemid 14242870)
77. I Wildhaber W Baumeister 1987 The cell envelope of Thermoproteus tenax: three-dimensional structure of the surface layer and its role in shape maintenance EMBO J 6 1475 1480 16453767 1:CAS:528:DyaL2sXkt1Snt7s%3D
78. AE Witter DA Hutchins A Butler GW Luther III 2000 Determination of conditional stability constants and kinetic constants for strong model Fe-binding ligands in seawater Mar Chem 69 1 17 10.1016/S0304-4203(99)00087-0 1:CAS:528:DC%2BD3cXhvVCktbw%3D (Pubitemid 30168021)
79. YI Wolf IB Rogozin NV Grishin EV Koonin 2002 Genome trees and the tree of life Trends Genet 18 472 479 12175808 10.1016/S0168-9525(02)02744-0 1:CAS:528:DC%2BD38Xmtleltbo%3D
80. D Wu P Hugenholtz K Mavromatis R Pukall E Dalin NN Ivanova V Kunin L Goodwin M Wu BJ Tindall SD Hooper A Pati A Lykidis S Spring IJ Anderson P D'haeseleer A Zemla M Singer A Lapidus M Nolan A Copeland C Han F Chen JF Cheng S Lucas C Kerfeld E Lang S Gronow P Chain D Bruce EM Rubin NC Kyrpides HP Klenk JA Eisen 2009 A phylogeny-driven genomic encyclopaedia of Bacteria and Archaea Nature 462 1056 1060 20033048 10.1038/nature08656 1:CAS:528:DC%2BD1MXhs1SktbbM
81. G Zellner E Stackebrandt H Kneifel P Messner UB Sleytr E Conway de Macario H-P Zabel KO Stetter J Winter 1989 Isolation and characterization of a thermophilic, sulfate reducing archaebacterium, Archaeoglobus fulgidus strain Z System Appl Microbiol 11 151 160 1:CAS:528:DyaL1MXkvFemtbo%3D