A two-step refolding of acid-denatured microbial transglutaminase escaping from the aggregation-prone intermediate

Biochemistry

Volumn 50, Issue 47, 2011, Pages 10390-10398

  Suzuki, Mototaka ^a,b   Yokoyama, Kei Ichi ^a   Lee, Young Ho ^b   Goto, Yuji ^b  

^a AJINOMOTO CO INC   (Japan)

^b OSAKA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID RESIDUES; CONCENTRATION-DEPENDENT; GLOBULAR ENZYMES; HIGH IONIC STRENGTH; KINETIC TRAPS; LOW IONIC STRENGTH; MICROBIAL TRANSGLUTAMINASE; MOLTEN GLOBULE STATE; NACL CONCENTRATION; NATIVE STATE; PI VALUES; REFOLDING; SECONDARY STRUCTURES; TERTIARY STRUCTURES;

AMINO ACIDS; DICHROISM; PHASE DIAGRAMS; SODIUM CHLORIDE;

IONIC STRENGTH;

8 ANILINO 1 NAPHTHALENESULFONIC ACID; MICROBIAL TRANSGLUTAMINASE; PROTEIN GLUTAMINE GAMMA GLUTAMYLTRANSFERASE; SODIUM CHLORIDE; UNCLASSIFIED DRUG;

ARTICLE; CIRCULAR DICHROISM; CONCENTRATION (PARAMETERS); CONFORMATIONAL TRANSITION; FLUORESCENCE; INCUBATION TIME; IONIC STRENGTH; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN TERTIARY STRUCTURE; STREPTOMYCES; STREPTOMYCES MOBARAENSIS;

ACIDS; BACTERIAL PROTEINS; HYDROGEN-ION CONCENTRATION; KINETICS; OSMOLAR CONCENTRATION; PROTEIN DENATURATION; PROTEIN FOLDING; STREPTOMYCES; TRANSGLUTAMINASES;

EID: 81855226607     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi2010619     Document Type: Article

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