A Novel Lipase from Pseudomonas fluorescens HU380: Gene Cloning, Overproduction, Renaturation-Activation, Two-Step Purification, and Characterization

Journal of Bioscience and Bioengineering

Volumn 96, Issue 3, 2003, Pages 242-249

  Kojima, Yuzo ^a   Kobayashi, Michihiko ^b   Shimizu, Sakayu ^c  

^a Amano Enzyme Inc   (Japan)

^b UNIVERSITY OF TSUKUBA   (Japan)

^c KYOTO UNIVERSITY   (Japan)

Author keywords

Lipase; Nucleotide sequence; Pseudomonas fluorescens; Purification; Refolding; Substrate specificity; Unsaturated fatty acid

Indexed keywords

AMINO ACIDS; DIALYSIS; ELECTROPHORESIS; ENZYMES; ESCHERICHIA COLI; FLUORESCENCE; UREA;

GENE CLONING;

GENES;

RECOMBINANT PROTEIN; TRIACYLGLYCEROL LIPASE;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL STRAIN; CELL INCLUSION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENE ACTIVATION; GENE LIBRARY; GENE OVEREXPRESSION; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PHYSICAL CHEMISTRY; PSEUDOMONAS FLUORESCENS; SERRATIA MARCESCENS; STRUCTURAL HOMOLOGY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; PSEUDOMONAS; PSEUDOMONAS FLUORESCENS; SERRATIA MARCESCENS;

EID: 0141922991     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1389-1723(03)80188-3     Document Type: Article

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