On-column refolding and purification of transglutaminase from Streptomyces fradiae expressed as inclusion bodies in Escherichia coli

Protein Expression and Purification

Volumn 51, Issue 2, 2007, Pages 179-186

  Liu, Xiao qiu ^a,b   Yang, Xiu qing ^a,b   Xie, Fu hong ^a,b   Song, Li ya ^a,b   Zhang, Guo qing ^a   Qian, Shi jun ^a  

^a INSTITUTE OF MICROBIOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

Gradient ion exchange chromatography; Inclusion body; Refolding; Transglutaminase

Indexed keywords

ARGININE; BOVINE SERUM ALBUMIN; GLYCEROL; MACROGOL DERIVATIVE; PROTEIN GLUTAMINE GAMMA GLUTAMYLTRANSFERASE; UREA;

ARTICLE; BIOSYNTHESIS; CELL INCLUSION; DRUG EFFECT; ENZYMOLOGY; ESCHERICHIA COLI; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR CLONING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN FOLDING; PROTEIN RENATURATION; SOLUBILITY; STREPTOMYCES;

ARGININE; CHROMATOGRAPHY, ION EXCHANGE; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GLYCEROL; INCLUSION BODIES; POLYETHYLENE GLYCOLS; PROTEIN FOLDING; PROTEIN RENATURATION; SERUM ALBUMIN, BOVINE; SOLUBILITY; STREPTOMYCES; TRANSGLUTAMINASES; UREA;

ESCHERICHIA COLI; STREPTOMYCES FRADIAE;

EID: 33751406658     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2006.07.011     Document Type: Article

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