Influence of bone remodelling rate on quantitative ultrasound parameters at the calcaneus and DXA BMDa of the hip and spine in middle-aged and elderly European men: The European Male Ageing Study (EMAS)

European Journal of Endocrinology

Volumn 165, Issue 6, 2011, Pages 977-986

  Boonen, Steven ^a,p   Pye, Stephen R ^b,p   O'Neill, Terence W ^b,p   Szulc, Pawel ^c   Gielen, Evelien ^a   Borghs, Herman ^a,p   Verschueren, Sabine ^a   Claessens, Frank ^a   Adams, Judith E ^b   Ward, Kate A ^b,d   Bartfai, Gyorgy ^e,p   Casanueva, Felipe ^f,p   Finn, Joseph D ^b,p   Forti, Gianni ^g,p   Giwercman, Aleksander ^h,p   Han, Thang S ⁱ   Huhtaniemi, Ilpo T ^j,p   Kula, Krzysztof ^k,p   Labrie, Fernand ^l   Lean, Michael E J ^m   Pendleton, Neil ^b   Punab, Margus ^n,p   Silman, Alan J ^o   Tajar, Abdelouahid ^b,p   Wu, Frederick C W ^b,p   Vanderschueren, Dirk ^a,p   Petrone, Luisa ^p   Corona, Giovanni ^p   Slowikowska Hilczer, Jolanta ^p   Walczak Jedrzejowska, Renata ^p   Silman, Alan ^p   Steer, Philip ^p   Lee, David ^p   Lage, Mary ^p   Castro, Ana I ^p   Foldesi, Imre ^p   Fejes, Imre ^p   Korrovitz, Paul ^p   Jiang, Min ^p   more..

^a UNIVERSITY OF LEUVEN   (Belgium)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

^c UNIVERSITÉ DE LYON   (France)

^d MRC HUMAN NUTRITION RESEARCH   (United Kingdom)

^e UNIVERSITY OF SZEGED   (Hungary)

^f UNIVERSITY OF SANTIAGO DE COMPOSTELA   (Spain)

^g UNIVERSITY OF FLORENCE   (Italy)

^h LUND UNIVERSITY HOSPITAL   (Sweden)

ⁱ ROYAL FREE AND UNIVERSITY COLLEGE MEDICAL SCHOOL   (United Kingdom)

^j IMPERIAL COLLEGE LONDON   (United Kingdom)

^k MEDICAL UNIVERSITY OF LODZ   (Poland)

^l LAVAL UNIVERSITY   (Canada)

^m UNIVERSITY OF GLASGOW   (United Kingdom)

ⁿ TARTU UNIVERSITY CLINICS   (Estonia)

^o Arthritis Research UK   (United Kingdom)

^p MANCHESTER ROYAL INFIRMARY   (United Kingdom)

more..

Author keywords

[No Author keywords available]

Indexed keywords

AMINO TERMINAL TELOPEPTIDE; CARBOXY TERMINAL TELOPEPTIDE; ESTRADIOL; PARATHYROID HORMONE; SEX HORMONE; SEX HORMONE BINDING GLOBULIN; SOMATOMEDIN C; TESTOSTERONE;

ADULT; AGE; AGED; ARTICLE; BODY HEIGHT; BODY WEIGHT; BONE DENSITY; BONE REMODELING; BONE TURNOVER; CALCANEUS; CROSS-SECTIONAL STUDY; DUAL ENERGY X RAY ABSORPTIOMETRY; ECHOGRAPHY; ETHNIC GROUP; EUROPEAN; HEALTH SURVEY; HIP; HORMONE DETERMINATION; HUMAN; LIFESTYLE; MALE; POPULATION RESEARCH; PRIORITY JOURNAL; QUESTIONNAIRE; SEASON; SPINE;

EID: 81555195436     PISSN: 08044643     EISSN: 1479683X     Source Type: Journal    
DOI: 10.1530/EJE-11-0353     Document Type: Article

References (67)

1. Szulc P, Garnero P, Munoz F, Marchand F & Delmas PD. Cross-sectional evaluation of bone metabolism in men. Journal of Bone and Mineral Research 2001 16 1642-1650. (doi:10.1359/jbmr.2001.16.9.1642)
2. Fatayerji D & Eastell R. Age-related changes in bone turnover in men. Journal of Bone and Mineral Research 1999 14 1203-1210. (doi:10.1359/jbmr.1999. 14.7.1203)
3. Khosla S, Melton LJ III, Atkinson EJ, O'Fallon WM, Klee GG &Riggs BL. Relationship of serum sex steroid levels and bone turnover markers with bone mineral density in men and women: a key role for bioavailable estrogen. Journal of Clinical Endocrinology and Metabolism 1998 83 2266-2274. (doi:10.1210/jc.83. 7.2266)
4. Wishart JM, Need AG, Horowitz M, Morris HA & Nordin BE. Effect of age on bone density and bone turnover in men. Clinical Endocrinology 1995 42 141-146. (doi:10.1111/j.1365-2265.1995.tb01854.x)
5. Chandani AK, Scariano JK, Glew RH, Clemens JD, Garry PJ &Baumgartner RN. Bone mineral density and serum levels of aminoterminal propeptides and cross-linked N-telopeptides of type I collagen in elderly men. Bone 2000 26 513-518. (doi:10.1016/S8756-3282(00)00262-3)
6. Orwoll ES, Bell NH, Nanes MS, Flessland KA, Pettinger MB, Mallinak NJ & Cain DF. Collagen N-telopeptide excretion in men: the effects of age and intrasubject variability. Journal of Clinical Endocrinology and Metabolism 1998 83 3930-3935. (doi:10.1210/jc.83.11.3930)
7. Gallagher JC, Kinyamu HK, Fowler SE, Dawson-Hughes B, Dalsky GP & Sherman SS. Calciotropic hormones and bone markers in the elderly. Journal of Bone and Mineral Research 1998 13 475-482. (doi:10.1359/jbmr.1998.13.3.475)
8. Amin S, Riggs BL, Melton LJ III, Achenbach SJ, Atkinson EJ &Khosla S. High serum IGFBP-2 is predictive of increased bone turnover in aging men and women. Journal of Bone and Mineral Research 2007 22 799-807. (doi:10.1359/jbmr.070306)
9. Szulc P, Kaufman JM & Delmas PD. Biochemical assessment of bone turnover and bone fragility in men. Osteoporosis International 2007 18 1451-1461. (doi:10.1007/s00198-007-0407-z)
10. Szulc P, Garnero P, Claustrat B, Marchand F, Duboeuf F &Delmas PD. Increased bone resorption in moderate smokers with low body weight: the Minos study. Journal of Clinical Endocrinology and Metabolism 2002 87 666-674. (doi:10.1210/jc.87.2.666)
11. Alvisa-Negrín J, González-Reimers E, Santolaria- Fernández F, García-Valdecasas-Campelo E, Valls MR, Pelazas-González R, Durán-Castellón MC & de Los Angeles Gómez-Rodríguez M. Osteopenia in alcoholics: effect of alcohol abstinence. Alcohol Alcoholism 2009 44 468-475. (doi:10.1093/alcalc/agp038)
12. Center JR, Nguyen TV, Sambrook PN & Eisman JA. Hormonal and biochemical parameters in the determination of osteoporosis in elderly men. Journal of Clinical Endocrinology and Metabolism 1999 84 3626-3635. (doi:10.1210/jc.84.10.3626)
13. Kenny AM, Gallagher JC, Prestwood KM, Gruman CA & Raisz LG. Bone density, bone turnover, and hormone levels in men over age 75. Journals of Gerontology. Series A: Biological Sciences Medical Sciences 1998 53A M419-M425. (doi:10.1093/gerona/53A.6.M419)
14. Schneider DL & Barrett-Connor EL. Urinary N-telopeptide levels discriminate normal, osteopenic and osteoporotic bone mineral density. Archives of Internal Medicine 1997 157 1241-1245. (doi:10.1001/archinte.157.11.1241)
15. Chaitou A, Boutroy S, Vilayphiou N, Munoz F, Delmas PD, Chapurlat R & Szulc P. Association between bone turnover rate and bone microarchitecture in men: the STRAMBO study. Journal of Bone and Mineral Research 2010 25 2313-2323. (doi:10.1002/jbmr.124)
16. Dennison E, Eastell R, Fall CHD, Kellingray S, Wood PJ & Cooper C. Determinants of bone loss in elderly men and women: a prospective population-based study. Osteoporosis International 1999 10 384-391. (doi:10.1007/s001980050244)
17. Szulc P, Montella A & Delmas PD. High bone turnover is associated with accelerated bone loss but not with increased fracture risk in men aged 50 and over: the prospective MINOS study. Annals of the Rheumatic Diseases 2008 67 1249-1255. (doi:10.1136/ard.2007.077941)
18. Bauer DC, Garnero P, Harrison SL, Cauley JA, Eastell R, Ensrud KE & Orwoll E. Biochemical markers of bone turnover, hip bone loss, and fracture in older men: the MrOS study. Journal of Bone and Mineral Research 2009 24 2032-2038. (doi:10.1359/jbmr.090526)
19. Lenora J, Gerdhem P, Obrant KJ & Ivaska KK. Bone turnover markers are correlated with quantitative ultrasound of the calcaneus: 5-year longitudinal data. Osteoporosis International 2009 20 1225-1232. (doi:10.1007/s00198-008- 0769-x)
20. Lappa V, Dontas IA, Trovas G, Constantelou E, Galanos A &Lyritis GP. Quantitative ultrasound is better correlated with bone mineral density and biochemical bone markers in elderly women. Clinical Rheumatology 2007 26 1067-1073. (doi:10.1007/s10067-006-0448-2)
21. Fiore CE, Pennisi P, Gibilaro M, Di Fazzio S, Impellizzieri D &Ramirez MG. Correlation of quantitative ultrasound of bone with biochemical markers of bone resorption in women with osteoporotic fractures. Journal of Clinical Densitometry 1999 2 231-239. (doi:10.1385/JCD:2:3:231)
22. Liu G & Peacock M. Age-related changes in serum undercarboxylated osteocalcin and its relationships with bone density, bone quality, and hip fracture. Calcified Tissue International 1998 62 286-289. (doi:10.1007/ s002239900432)
23. Matsushita R, Yamamoto I, Takada M, Hamanaka Y, Yuh I &Morita R. Comparison of various biochemical measurements with bone mineral densitometry and quantitative ultrasound for the assessment of vertebral fracture. Journal of Bone and Mineral Metabolism 2000 18 158-164. (doi:10.1007/s007740050107)
24. McLean RR, Booth SL, Kiel DP, Broe KE, Gagnon DR, Tucker KL, Cupples LA & Hannan MT. Association of dietary and biochemical measures of vitamin K with quantitative ultrasound of the heel in men and women. Osteoporosis International 2006 17 600-607. (doi:10.1007/s00198-005-0022-9)
25. Krieg MA, Cornuz J, Jacquet AF, Thiébaud D & Burckhardt P. Influence of anthropometric parameters and biochemical markers of bone metabolism on quantitative ultrasound of bone in the institutionalized elderly. Osteoporosis International 1998 8 115-120. (doi:10.1007/BF02672506)
26. Lee DM, O'Neill TW, Pye SR, Silman AJ, Finn JD, Pendleton N, Tajar A, Bartfai G, Casanueva F, Forti G, Giwercman A, Huhtaniemi IT, Kula K, Punab M, Boonen S, Vanderschueren D, Wu FC & EMAS Study Group . The European Male Ageing Study (EMAS): design, methods and recruitment. International Journal of Andrology 2009 32 11-24. (doi:10.1111/j.1365-2605.2008.00879.x)
27. Labrie F, Bélanger A, Bélanger P, Bérubé R, Martel C, Cusan L, Gomez J, Candas B, Castiel I, Chaussade V, Deloche C & Leclaire J. Androgen glucuronides, instead of testosterone, as the new markers of androgenic activity in women. Journal of Steroid Biochemistry and Molecular Biology 2006 99 182-188. (doi:10.1016/j.jsbmb.2006.02.004)
28. Labrie F, Bélanger A, Bélanger P, Bérubé R, Martel C, Cusan L, Gomez J, Candas B, Chaussade V, Castiel I, Deloche C & Leclaire J. Metabolism of DHEA in postmenopausal women following percutaneous administration. Journal of Steroid Biochemistry and Molecular Biology 2007 103 178-188. (doi:10.1016/j.jsbmb.2006.09.034)
29. Wu FC, Tajar A, Pye SR, Silman AJ, Finn JD, O'Neill TW, Bartfai G, Casanueva F, Forti G, Giwercman A, Huhtaniemi IT, Kula K, Punab M, Boonen S & Vanderschueren D. Hypothalamic-pituitary-testicular axis disruptions in older men are differentially linked to age and modifiable risk factors. Journal of Clinical Endocrinology and Metabolism 2008 93 2737-2745. (doi:10.1210/jc. 2007-1972)
30. Vermeulen A, Verdonck L & Kaufman JM. A critical evaluation of simple methods for the estimation of free testosterone in serum. Journal of Clinical Endocrinology and Metabolism 1999 84 3666-3672. (doi:10.1210/jc.84.10.3666)
31. Van Pottelbergh I, Goemaere S & Kaufman JM. Bioavailable estradiol and an aromatase gene polymorphism are determinants of bone mineral density changes in men over 70 years of age. Journal of Clinical Endocrinology and Metabolism 2003 88 3075-3081. (doi:10.1210/jc.2002-021691)
32. Garnero P, Vergnaud P & Hoyle N. Evaluation of a fully automated serum assay for total N-terminal propeptide of type I collagen in postmenopausal osteoporosis. Clinical Chemistry 2008 54 188-196. (doi:10.1373/clinchem.2007. 094953)
33. Glüer CC, Blake G, Lu Y, Blunt BA, Jergas M & Genant HK. Accurate assessment of precision errors: how to measure the reproducibility of bone densitometry techniques. Osteoporosis International 1995 5 262-270. (doi:10.1007/BF01774016)
34. Reid DM, MacKay I & Wilkinson S. Cross-calibration of dual-energy X-ray densitometers for a large, multi-center genetic study of osteoporosis. Osteoporosis International 2006 17 125-132. (doi:10.1007/s00198-005-1936-y)
35. Gundberg CM, Looker AC, Nieman SD & Calvo MS. Patterns of osteocalcin and bone specific alkaline phosphatase by age, gender, and race or ethnicity. Bone 2002 31 703-708. (doi:10.1016/S8756-3282(02)00902-X)
36. Szulc P, Claustrat B, Marchand F & Delmas PD. Increased risk of falls and increased bone resorption in elderly men with partial androgen deficiency: the MINOS study. Journal of Clinical Endocrinology and Metabolism 2003 88 5240-5247. (doi:10.1210/jc.2003-030200)
37. Theiler R, Stähelin HB, Kränzlin M, Tyndall A & Bischoff HA. High bone turnover in the elderly. Archives of Physical Medicine and Rehabilitation 1999 80 485-489. (doi:10.1016/S0003-9993(99)90186-7)
38. Chen JS, Cameron ID, Cumming RG, Lord SR, March LM, Sambrook PN, Simpson JM & Seibel MJ. Effect of age-related chronic immobility on markers of bone turnover. Journal of Bone and Mineral Research 2006 21 324-331. (doi:10.1359/JBMR.051014)
39. Szulc P & Delmas PD. Biochemical markers of bone turnover: potential use in the investigation and management of postmenopausal osteoporosis. Osteoporosis International 2008 19 1683-1704. (doi:10.1007/s00198-008-0660-9)
40. Falahati-Nini A, Riggs BL, Atkinson EJ, O'Fallon WM, Eastell R &Khosla S. Relative contributions of testosterone and estrogen in regulating bone resorption and formation in normal elderly men. Journal of Clinical Investigation 2000 106 1553-1560. (doi:10.1172/JCI10942)
41. Leder BZ, LeBlanc KM, Schoenfeld DA, Eastell R & Finkelstein JS. Differential effects of androgens and estrogens on bone turnover in normal men. Journal of Clinical Endocrinology and Metabolism 2003 88 204-210. (doi:10.1210/jc.2002-021036)
42. Szulc P, Munoz F, Claustrat B, Garnero P, Marchand F, Duboeuf F &Delmas PD. Bioavailable estradiol may be an important determinant of osteoporosis in men: the MINOS study. Journal of Clinical Endocrinology and Metabolism 2001 86 192-199. (doi:10.1210/jc.86.1.192)
43. Gennari L, Merlotti D, Martini G, Gonnelli S, Franci B, Campagna S, Lucani B, Dal Canto N, Valenti R, Gennari C &Nuti R. Longitudinal association between sex hormone levels, bone loss, and bone turnover in elderly men. Journal of Clinical Endocrinology and Metabolism 2003 88 5327-5333. (doi:10.1210/jc.2003-030736)
44. Goemaere S, Van Pottelbergh I, Zmierczak H, Toye K, Daems M, Demuynck R, Myny H, De Bacquer D & Kaufman JM. Inverse association between bone turnover rate and bone mineral density in community-dwelling men >70 years of age: no major role of sex steroid status. Bone 2001 29 286-291. (doi:10.1016/S8756- 3282(01)00503-8)
45. Wang C, Eyre DR, Clark R, Kleinberg D, Newman C, Iranmanesh A, Veldhuis J, Dudley RE, Berman N, Davidson T, Barstow TJ, Sinow R, Alexander G & Swerdloff RS. Sublingual testosterone replacement improves muscle mass and strength, decreases bone resorption, and increases bone formation markers in hypogonadal men-a clinical research center study. Journal of Clinical Endocrinology and Metabolism 1996 81 3654-3662. (doi:10.1210/jc.81.10.3654)
46. Kuchuk NO, van Schoor NM, Pluijm SM, Smit JH, de Ronde W &Lips P. The association of sex hormone levels with quantitative ultrasound, bone mineral density, bone turnover and osteoporotic fractures in older men and women. Clinical Endocrinology 2007 67 295-303. (doi:10.1111/j.1365-2265.2007.02882.x)
47. Scopacasa F, Horowitz M, Wishart JM, Morris HA, Chatterton BE &Need AG. The relation between bone density, free androgen index, and estradiol in men 60 to 70 years old. Bone 2000 27 145-149. (doi:10.1016/S8756-3282(00)00299-4)
48. Legrand E, Hedde C, Gallois Y, Degasne I, Boux de Casson F, Mathieu E, Baslé MF, Chappard D & Audran M. Osteoporosis in men: a potential role for the sex hormones binding globulin. Bone 2001 29 90-95. (doi:10.1016/S8756-3282(01)00478-1)
49. Gillberg P, Johansson AG & Ljunghall S. Decreased estradiol levels and free androgen index and elevated sex hormone-binding globulin levels in male idiopathic osteoporosis. Calcified Tissue International 1999 64 209-213. (doi:10.1007/s002239900604)
50. Mellstrom D, Vandenput L, Mallmin H, Holmberg AH, Lorentzon M, Odén A, Johansson H, Orwoll ES, Labrie F, Karlsson MK, Ljunggren O & Ohlsson C. Older men with low serum estradiol and high serum SHBG have an increased risk of fractures. Journal of Bone and Mineral Research 2008 23 1552-1560. (doi:10.1359/jbmr.080518)
51. Vanderschueren D, Pye SR, Venken K, Borghs H, Gaytant J, Huhtaniemi IT, Adams JE, Ward KA, Bartfai G, Casanueva FF, Finn JD, Forti G, Giwercman A, Han TS, Kula K, Labrie F, Lean ME, Pendleton N, Punab M, Silman AJ, Wu FC, O'Neill TW, Boonen S &EMAS Study Group . Gonadal sex steroid status and bone health in middle aged and elderly European men. Osteoporosis International 2010 21 1331-1339. (doi:10.1007/s00198-009-1144-2)
52. Ooms ME, Lips P, Roos JC, van der Vijgh WJ, Popp-Snijders C, Bezemer PD & Bouter LM. Vitamin D status and sex hormone binding globulin: determinants of bone turnover and bone mineral density in elderly women. Journal of Bone and Mineral Research 1995 10 1177-1184. (doi:10.1002/jbmr.5650100806)
53. Handelsman DJ. Update in andrology. Journal of Clinical Endocrinology and Metabolism 2007 92 4505-4511. (doi:10.1210/jc.2007-1431)
54. Morley JE, Patrick P & Perry HM III. Evaluation of assays available to measure free testosterone. Metabolism 2002 51 554-559. (doi:10.1053/meta. 2002.31975)
55. Szulc P, Claustrat B, Munoz F, Marchand F & Delmas PD. Assessment of the role of 17beta-oestradiol in bone metabolism in men: does the assay technique matter? The MINOS study Clinical Endocrinology 2004 61 447-457. (doi:10.1111/j.1365-2265.2004.02117.x)
56. Vanbillemont G, Bogaert V, De Bacquer D, Lapauw B, Goemaere S, Toye K, Van Steen K, Taes Y & Kaufman JM. Polymorphisms of the SHBG gene contribute to the interindividual variation of sex steroid hormone blood levels in young, middle-aged and elderly men. Clinical Endocrinology 2009 70 303-310. (doi:10.1111/j.1365-2265.2008.03365.x)
57. Terasaki T, Nowlin DM & Pardridge WM. Differential binding of testosterone and estradiol to isoforms of sex hormone-binding globulin: selective alteration of estradiol binding in cirrhosis. Journal of Clinical Endocrinology and Metabolism 1988 67 639-643. (doi:10.1210/jcem-67-4-639)
58. Eriksson AL, Lorentzon M, Mellström D, Vandenput L, Swanson C, Andersson N, Hammond GL, Jakobsson J, Rane A, Orwoll ES, Ljunggren O, Johnell O, Labrie F, Windahl SH & Ohlsson C. SHBG gene promoter polymorphisms in men are associated with serum sex hormone-binding globulin, androgen and androgen metabolite levels, and hip bone mineral density. Journal of Clinical Endocrinology and Metabolism 2006 91 5029-5037. (doi:10.1210/jc.2006-0679)
59. Södergård R, Bäckström T, Shanbhag V & Carstensen H. Calculation of free and bound fractions of testosterone and estradiol-17 beta to human plasma proteins at body temperature. Journal of Steroid Biochemistry and Molecular Biology 1982 16 801-810. (doi:10.1016/0022- 4731(82)90038-3)
60. Szulc P, Munoz F, Marchand F, Chapuy MC & Delmas PD. Role of vitamin D and parathyroid hormone in the regulation of bone turnover and bone mass in men: the MINOS study. Calcified Tissue International 2003 73 520-530. (doi:10.1007/s00223-002-2103-5)
61. Boonen S, Bischoff-Ferrari HA, Cooper C, Lips P, Ljunggren O, Meunier PJ & Reginster JY. Addressing the musculoskeletal components of fracture risk with calcium and vitamin D: a review of the evidence. Calcified Tissue International 2006 78 257-270. (doi:10.1007/s00223-005-0009-8)
62. van Coeverden SC, Netelenbos JC, de Ridder CM, Roos JC, Popp-Snijders C & Delemarre-van de Waal HA. Bone metabolism markers and bone mass in healthy pubertal boys and girls. Clinical Endocrinology 2002 57 107-116. (doi:10.1046/j.1365-2265.2002.01573.x)
63. Johansen JS, Giwercman A, Hartwell D, Nielsen CT, Price PA, Christiansen C & Skakkebaek NE. Serum bone Gla-protein as a marker of bone growth in children and adolescents: correlation with age, height, serum insulin-like growth factor I, and serum testosterone. Journal of Clinical Endocrinology and Metabolism 1988 67 273-278. (doi:10.1210/jcem-67-2-273)
64. Nicholson PH, Müller R, Lowet G, Cheng XG, Hildebrand T, Rüegsegger P, van der Perre G, Dequeker J & Boonen S. Do quantitative ultrasound measurements reflect structure independently of density in human vertebral cancellous bone? Bone 1998 23 425-431. (doi:10.1016/S8756-3282(98) 00128-8)
65. Khaw KT, Reeve J, Luben R, Bingham S, Welch A, Wareham N, Oakes S & Day N. Prediction of total and hip fracture risk in men and women by quantitative ultrasound of the calcaneus: EPIC-Norfolk prospective population study. Lancet 2004 363 197-202. (doi:10.1016/S0140-6736(03)15325-1)
66. Bauer DC, Ewing SK, Cauley JA, Ensrud KE, Cummings SR &Orwoll ES. Quantitative ultrasound predicts hip and non-spine fracture in men: the MrOS study. Osteoporosis International 2007 18 771-777. (doi:10.1007/s00198-006-0317- 5)
67. Gonnelli S, Cepollaro C, Gennari L, Montagnani A, Caffarelli C, Merlotti D, Rossi S, Cadirni A & Nuti R. Quantitative ultrasound and dual-energy X-ray absorptiometry in the prediction of fragility fracture in men. Osteoporosis International 2005 16 963-968. (doi:10.1007/s00198-004-1771-6)