Impacts of two perfluorinated compounds (PFOS and PFOA) on human hepatoma cells: Cytotoxicity but no genotoxicity?

International Journal of Hygiene and Environmental Health

Volumn 214, Issue 6, 2011, Pages 493-499

  Florentin, Arnaud ^a   Deblonde, Tiphanie ^a   Diguio, Nathalie ^a   Hautemaniere, Alexis ^a   Hartemann, Philippe ^a,b  

^a UNIVERSITÉ DE LORRAINE   (France)

^b INSERM   (France)

Author keywords

HepG2; Human hepatoma cells; Micronucleus; MTT assay; Perfluorinated compounds; PFC; PFOA; PFOS; Single cell gel electrophoresis

Indexed keywords

ALKANESULFONIC ACID; FLUOROCARBON; OCTANOIC ACID DERIVATIVE; PERFLUOROOCTANESULFONIC ACID; PERFLUOROOCTANOIC ACID; REACTIVE OXYGEN METABOLITE;

ARTICLE; CELL STRAIN HEPG2; COMET ASSAY; DNA STRAND BREAKAGE; DRUG EFFECT; HUMAN; MICRONUCLEUS TEST;

ALKANESULFONIC ACIDS; COMET ASSAY; DNA BREAKS; FLUOROCARBONS; HEP G2 CELLS; HUMANS; MICRONUCLEUS TESTS; OCTANOIC ACIDS; REACTIVE OXYGEN SPECIES;

RODENTIA;

EID: 81555195254     PISSN: 14384639     EISSN: 1618131X     Source Type: Journal    
DOI: 10.1016/j.ijheh.2011.05.010     Document Type: Article

References (40)

1. Andersen M.E., Butenhoff J.L., Chang S.C., Farrar D.G., Kennedy G.L., Lau C., Olsen G.W., Seed J., Wallace K.B. Perfluoroalkyl acids and related chemistries-toxicokinetics and modes of action. Toxicol. Sci. 2008, 102:3-14.
2. Diamond L., Kruszewski F., Aden D.P., Knowles B.B., Baird W.M. Metabolic activation of benzo[a]pyrene by a human hepatoma cell line. Carcinogenesis 1980, 1:871-875.
3. Emmett E.A., Zhang H., Shofer F.S., Freeman D., Rodway N.V., Desai C., Shaw L.M. Community exposure to perfluorooctanoate: relationships between serum levels and certain health parameters. J. Occup. Environ. Med. 2006, 48:771-779.
4. Ericson I., Gomez M., Nadal M., van Bavel B., Lindstrom G., Domingo J.L. Perfluorinated chemicals in blood of residents in Catalonia (Spain) in relation to age and gender: a pilot study. Environ. Int. 2007, 33:616-623.
5. Eriksen K.T., Sorensen M., McLaughlin J.K., Lipworth L., Tjonneland A., Overvad K., Raaschou-Nielsen O. Perfluorooctanoate and perfluorooctanesulfonate plasma levels and risk of cancer in the general Danish population. J. Natl. Cancer Inst. 2009, 101:605-609.
6. Eriksen K.T., Raaschou-Nielsen O., Sorensen M., Roursgaard M., Loft S., Moller P. Genotoxic potential of the perfluorinated chemicals PFOA, PFOS, PFBS, PFNA and PFHxA in human HepG2 cells. Mut. Res. 2010, 700:39-43.
7. Giesy J.P., Kannan K. Global distribution of perfluorooctane sulfonate in wildlife. Environ. Sci. Technol. 2001, 35:1339-1342.
8. Hartmann A., Elhajouji A., Kiskinis E., Poetter F., Martus H., Fjallman A., Frieauff W., Suter W. Use of the alkaline comet assay for industrial genotoxicity screening: comparative investigation with the micronucleus test. Food Chem. Toxicol. 2001, 39:843-858.
9. Holzer J., Midasch O., Rauchfuss K., Kraft M., Reupert R., Angerer J., Kleeschulte P., Marschall N., Wilhelm M. Biomonitoring of perfluorinated compounds in children and adults exposed to perfluorooctanoate-contaminated drinking water. Environ. Health Perspect. 2008, 116:651-657.
10. Holzer J., Goen T., Rauchfuss K., Kraft M., Angerer J., Kleeschulte P., Wilhelm M. One-year follow-up of perfluorinated compounds in plasma of German residents from Arnsberg formerly exposed to PFOA-contaminated drinking water. Int. J. Hyg. Environ. Health 2009, 212:499-504.
11. Hu X.Z., Hu D.C. Effects of perfluorooctanoate and perfluorooctane sulfonate exposure on hepatoma Hep G2 cells. Arch. Toxicol. 2009, 83:851-861.
12. Kannan K., Corsolini S., Falandysz J., Fillmann G., Kumar K.S., Loganathan B.G., Mohd M.A., Olivero J., Van Wouwe N., Yang J.H., Aldoust K.M. Perfluorooctanesulfonate and related fluorochemicals in human blood from several countries. Environ. Sci. Technol. 2004, 38:4489-4495.
13. Karrman A., Ericson I., van Bavel B., Darnerud P.O., Aune M., Glynn A., Lignell S., Lindstrom G. Exposure of perfluorinated chemicals through lactation: levels of matched human milk and serum and a temporal trend, 1996-2004, in Sweden. Environ. Health Perspect. 2007, 115:226-230.
14. Kennedy G.L., Butenhoff J.L., Olsen G.W., O'Connor J.C., Seacat A.M., Perkins R.G., Biegel L.B., Murphy S.R., Farrar D.G. The toxicology of perfluorooctanoate. Crit. Rev. Toxicol. 2004, 34:351-384.
15. Kirsch-Volders M., Sofuni T., Aardema S., Albertini M., Eastmond D., Fenech M., Ishidate M., Lorge E., Norppa H., Surrallés J., von der Hude W., Wakata A. Report from the in vitro micronucleus assay Working Group. Environ. Mol. Mutagen. 2000, 35:167-172.
16. Knasmuller S., Parzefall W., Sanyal R., Ecker S., Schwab C., Uhl M., Mersch-Sundermann V., Williamson G., Hietsch G., Langer T., Darroudi F., Natarajan A.T. Use of metabolically competent human hepatoma cells for the detection of mutagens and antimutagens. Mutat. Res. 1998, 402:185-202.
17. Knasmuller S., Mersch-Sundermann V., Kevekordes S., Darroudi F., Huber W.W., Hoelzl C., Bichler J., Majer B.J. Use of human-derived liver cell lines for the detection of environmental and dietary genotoxicants: current state of knowledge. Toxicology 2004, 198:315-328.
18. Lau C., Anitole K., Hodes C., Lai D., Pfahles-Hutchens A., Seed J. Perfluoroalkyl acids: a review of monitoring and toxicological findings. Toxicol. Sci. 2007, 99:366-394.
19. Levitt D., Liss A. Toxicity of perfluorinated fatty acids for human and murine B cell lines. Toxicol. Appl. Pharmacol. 1986, 86:1-11.
20. Martin J.W., Smithwick M.M., Braune B.M., Hoekstra P.F., Muir D.C., Mabury S.A. Identification of long-chain perfluorinated acids in biota from the Canadian Arctic. Environ. Sci. Technol. 2004, 38:373-380.
21. Melzer D., Rice N., Depledge M.H., Henley W.E., Galloway T.S. Association between serum perfluorooctanoic acid (PFOA) and thyroid disease in the U.S. National Health and Nutrition Examination Survey. Environ. Health Perspect. 2010, 118:686-692.
22. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 1983, 65:55-63.
23. Natarajan A.T., Darroudi F. Use of human hepatoma cells for in vitro metabolic activation of chemical mutagens/carcinogens. Mutagenesis 1991, 6(5):399-403.
24. Olsen G.W., Burris J.M., Burlew M.M., Mandel J.H. Epidemiologic assessment of worker serum perfluorooctanesulfonate (PFOS) and perfluorooctanoate (PFOA) concentrations and medical surveillance examinations. J. Occup. Environ. Med. 2003, 45:260-270.
25. Olsen G.W., Burlew M.M., Marshall J.C., Burris J.M., Mandel J.H. Analysis of episodes of care in a perfluorooctanesulfonyl fluoride production facility. J. Occup. Environ. Med. 2004, 46:837-846.
26. Olsen G.W., Huang H.Y., Helzlsouer K.J., Hansen K.J., Butenhoff J.L., Mandel J.H. Historical comparison of perfluorooctanesulfonate, perfluorooctanoate, and other fluorochemicals in human blood. Environ. Health Perspect. 2005, 113:539-545.
27. Olsen G.W., Butenhoff J.L., Zobel L.R. Perfluoroalkyl chemicals and human fetal development: an epidemiologic review with clinical and toxicological perspectives. Reprod. Toxicol. 2009, 27(3-4):212-230.
28. Panaretakis T., Shabalina I.G., Grander D., Shoshan M.C., DePierre J.W. Reactive oxygen species and mitochondria mediate the induction of apoptosis in human hepatoma HepG2 cells by the rodent peroxisome proliferator and hepatocarcinogen, perfluorooctanoic acid. Toxicol. Appl. Pharmacol. 2001, 173:56-64.
29. Quinete N., Orata F., Maes A., Gehron M., Bauer K.H., Moreira I., Wilken R.D. Degradation studies of new substitutes for perfluorinated surfactants. Arch. Environ. Contam. Toxicol. 2010, 59:20-30.
30. Sassa S., Iwasa F., Galbraith R. The effect of dimethyl sulfoxide on heme synthesis and the acute phase reaction in human HepG2 hepatoma cells. Adv. Exp. Med. Biol. 1989, 271:103-114.
31. Scudiero D.A., Shoemaker R.H., Paull K.D., Monks A., Tierney S., Nofziger T.H., Currens M.J., Seniff D., Boyd M.R. Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. Cancer Res. 1988, 48:4827-4833.
32. Shabalina I.G., Panaretakis T., Bergstrand A., DePierre J.W. Effects of the rodent peroxisome proliferator and hepatocarcinogen, perfluorooctanoic acid, on apoptosis in human hepatoma HepG2 cells. Carcinogenesis 1999, 20:2237-2246.
33. Tice R.R., Agurell E., Anderson D., Burlinson B., Hartmann A., Kobayashi H., Miyamae Y., Rojas E., Ryu J.C., Sasaki Y.F. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ. Mol. Mutagen. 2000, 35:206-221.
34. Uhl M., Helma C., Knasmuller S. Evaluation of the single cell gel electrophoresis assay with human hepatoma (Hep G2) cells. Mutat. Res. 2000, 468:213-225.
35. Valentin-Severin I., Le Hegarat L., Lhuguenot J.C., Le Bon A.M., Chagnon M.C. Use of HepG2 cell line for direct or indirect mutagens screening: comparative investigation between comet and micronucleus assays. Mutat. Res. 2003, 536:79-90.
36. Volkel W., Genzel-Boroviczeny O., Demmelmair H., Gebauer C., Koletzko B., Twardella D., Raab U., Fromme H. Perfluorooctane sulphonate (PFOS) and perfluorooctanoic acid (PFOA) in human breast milk: results of a pilot study. Int. J. Hyg. Environ. Health 2008, 211:440-446.
37. Wilhelm M., Kraft M., Rauchfuss K., Holzer J. Assessment and management of the first German case of a contamination with perfluorinated compounds (PFC) in the Region Sauerland, North Rhine-Westphalia. J. Toxicol. Environ. Health A 2008, 71:725-733.
38. Wilhelm M., Angerer J., Fromme H., Holzer J. Contribution to the evaluation of reference values for PFOA and PFOS in plasma of children and adults from Germany. Int. J. Hyg. Environ. Health 2009, 212:56-60.
39. White S.S., Fenton S.E., Hines E.P. Endocrine disrupting properties of perfluorooctanoic acid. J. Steroid Biochem. Mol. Biol 2011.
40. Yao X., Zhong L. Genotoxic risk and oxidative DNA damage in HepG2 cells exposed to perfluorooctanoic acid. Mutat. Res. 2005, 587:38-44.