Cloning and expression of a phloretin hydrolase gene from Eubacterium ramulus and characterization of the recombinant enzyme

Applied and Environmental Microbiology

Volumn 70, Issue 10, 2004, Pages 6131-6137

  Schoefer, Lilian ^a   Braune, Annett ^a   Blaut, Michael ^a  

^a GERMAN INSTITUTE OF HUMAN NUTRITION   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BIOSYNTHESIS; CARBON; CHEMICAL BONDS; CLONING; DEGRADATION; ELECTROPHORESIS; FILTRATION; GENES; HYDROLYSIS; PH EFFECTS; SCREENING;

GEL ELECTROPHORESIS; GEL FILTRATION; GENE ENCODING; GENE EXPRESSION;

ENZYMES;

1,10 PHENANTHROLINE; 1,3 DIPHENYL 1,3 PROPANEDIONE; 2,4 DIACETYLPHLOROGLUCINOL; ACETIC ACID DERIVATIVE; BIPHENYL DERIVATIVE; CHALCONE DERIVATIVE; CHALCONE OXIDE; COPPER CHLORIDE; DIMER; FLAVONOID; HESPERIDIN; HYDROLASE; N BROMOSUCCINIMIDE; N ETHYLMALEIMIDE; NEOHESPERIDIN DIHYDROCHALCONE; PHLORETIC ACID; PHLORETIN; PHLORETIN HYDROLASE; PHLORIZIN; PHLOROGLUCINOL; PHLOROGLUCINOL DERIVATIVE; PROPANE; PROPIONIC ACID DERIVATIVE; RECOMBINANT ENZYME; UNCLASSIFIED DRUG;

BACTERIUM; ENZYME;

AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; DEGRADATION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME MECHANISM; ENZYME PURIFICATION; ESCHERICHIA COLI; EUBACTERIUM; EUBACTERIUM RAMULUS; GEL FILTRATION; GENE EXPRESSION; GENE SEQUENCE; HYDROLYSIS; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; OPERON; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROMOTER REGION; PROTEIN EXPRESSION; PSEUDOMONAS FLUORESCENS; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; DNA, BACTERIAL; ESCHERICHIA COLI; EUBACTERIUM; GENES, BACTERIAL; HYDROLASES; KINETICS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PHLORETIN; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SEQUENCE HOMOLOGY, NUCLEIC ACID;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUBACTERIUM RAMULUS; PROKARYOTA; PSEUDOMONAS; PSEUDOMONAS FLUORESCENS; UNCULTURED ACTINOMYCETE;

EID: 8144230744     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.70.10.6131-6137.2004     Document Type: Article

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