Two-color STED microscopy of living synapses using a single laser-beam pair

Biophysical Journal

Volumn 101, Issue 10, 2011, Pages 2545-2552

  Tønnesen, Jan ^a,b   Nadrigny, Fabien ^a,b   Willig, Katrin I ^c   Wedlich Söldner, Roland ^d   Nägerl, U Valentin ^a,b  

^a UNIVERSITÉ DE BORDEAUX   (France)

^b CNRS   (France)

^c MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

^d MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; FLUORESCENT DYE; GREEN FLUORESCENT PROTEIN; PHOTOPROTEIN; YELLOW FLUORESCENT PROTEIN, BACTERIA;

ANIMAL; ARTICLE; CELL SURVIVAL; DENDRITIC SPINE; FLUORESCENCE MICROSCOPY; LASER; METABOLISM; METHODOLOGY; MOUSE; SYNAPSE; TIME LAPSE IMAGING;

ANIMALS; BACTERIAL PROTEINS; CELL SURVIVAL; DENDRITIC SPINES; FLUORESCENT DYES; GREEN FLUORESCENT PROTEINS; LASERS; LUMINESCENT PROTEINS; MICE; MICROSCOPY, FLUORESCENCE; SYNAPSES; TIME-LAPSE IMAGING;

ALEXA;

EID: 81255164785     PISSN: 00063495     EISSN: 15420086     Source Type: Journal    
DOI: 10.1016/j.bpj.2011.10.011     Document Type: Article

References (24)

1. S.W. Hell, and J. Wichmann Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy Opt. Lett. 19 1994 780 782
2. T.A. Klar, and S. Jakobs S.W. Hell Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission Proc. Natl. Acad. Sci. USA 97 2000 8206 8210
3. 2+ channel clustering, and vesicle release Science 312 2006 1051 1054
4. J.J. Sieber, and K.I. Willig T. Lang The SNARE motif is essential for the formation of syntaxin clusters in the plasma membrane Biophys. J. 90 2006 2843 2851
5. V. Westphal, and S.O. Rizzoli S.W. Hell Video-rate far-field optical nanoscopy dissects synaptic vesicle movement Science 320 2008 246 249
6. U.V. Nägerl, and K.I. Willig T. Bonhoeffer Live-cell imaging of dendritic spines by STED microscopy Proc. Natl. Acad. Sci. USA 105 2008 18982 18987
7. N.T. Urban, and K.I. Willig U.V. Nägerl STED nanoscopy of actin dynamics in synapses deep inside living brain slices Biophys. J. 101 2011 1277 1284
8. G. Donnert, and J. Keller S.W. Hell Two-color far-field fluorescence nanoscopy Biophys. J. 92 2007 L67 L69
9. L. Meyer, and D. Wildanger S.W. Hell Dual-color STED microscopy at 30-nm focal-plane resolution Small 4 2008 1095 1100
10. J. Bückers, and D. Wildanger S.W. Hell Simultaneous multi-lifetime multi-color STED imaging for colocalization analyses Opt. Express 19 2011 3130 3143
11. K.I. Willig, and A.C. Stiel S.W. Hell Dual-label STED nanoscopy of living cells using photochromism Nano Lett. 11 2011 3970 3973
12. R. Neher, and E. Neher Optimizing imaging parameters for the separation of multiple labels in a fluorescence image J. Microsc. 213 2004 46 62
13. T. Zimmermann, J. Rietdorf, and R. Pepperkok Spectral imaging and its applications in live cell microscopy FEBS Lett. 546 2003 87 92
14. U.V. Nägerl, and T. Bonhoeffer Imaging living synapses at the nanoscale by STED microscopy J. Neurosci. 30 2010 9341 9346
15. IMSpector. www.max-planck-innovation.de/de/industrie/technologieangebote/ software/.
16. B.H. Gähwiler Organotypic monolayer cultures of nervous tissue J. Neurosci. Methods 4 1981 329 342
17. Reference deleted in proof.
18. E. Picht, and A.V. Zima D.M. Bers SparkMaster: automated calcium spark analysis with ImageJ Am. J. Physiol. Cell Physiol. 293 2007 C1073 C1081
19. Unmixing. http://rsbweb.nih.gov/ij/plugins/spectral-unmixing.html.
20. K.M. Harris, and S.B. Kater Dendritic spines: cellular specializations imparting both stability and flexibility to synaptic function Annu. Rev. Neurosci. 17 1994 341 371
21. K.I. Willig, and B. Harke S.W. Hell STED microscopy with continuous wave beams Nat. Methods 4 2007 915 918
22. D. Toomre, and J. Bewersdorf A new wave of cellular imaging Annu. Rev. Cell Dev. Biol. 26 2010 285 314
23. K.I. Willig, and R.R. Kellner S.W. Hell Nanoscale resolution in GFP-based microscopy Nat. Methods 3 2006 721 723
24. B.R. Rankin, and G. Moneron S.W. Hell Nanoscopy in a living multicellular organism expressing GFP Biophys. J. 100 2011 L63 L65